Abstract
Circulating cancer stem cells (CCSCs), a rare circulating tumor cell (CTC) type, recently arose as a useful resource for monitoring and characterizing both cancers and their metastatic derivatives. However, due to the scarcity of CCSCs among hematologic cells in the blood and the complexity of the phenotype confirmation process, CCSC research can be extremely challenging. Hence, we report a nanoparticle-mediated Raman imaging method for CCSC characterization which profiles CCSCs based on their surface marker expression phenotypes. We have developed an integrated combinatorial Raman-Active Nanoprobe (RAN) system combined with a microfluidic chip to successfully process complete blood samples. CCSCs and CTCs were detected (90% efficiency) and classified in accordance with their respective surface marker expression via completely distinct Raman signals of RANs. Selectively isolated CCSCs (93% accuracy) were employed for both in vitro and in vivo tumor phenotyping to identify the tumorigenicity of the CCSCs. We utilized our new method to predict metastasis by screening blood samples from xenograft models, showing that upon CCSC detection, all subjects exhibited liver metastasis. Having highly efficient detection and noninvasive isolation capabilities, we have demonstrated that our RAN-based Raman imaging method will be valuable for predicting cancer metastasis and relapse via CCSC detection. Moreover, the exclusion of peak overlapping in CCSC analysis with our Raman imaging method will allow to expand the RAN families for various cancer types, therefore, increasing therapeutic efficacy by providing detailed molecular features of tumor subtypes.
| Original language | English |
|---|---|
| Pages (from-to) | 372-382 |
| Number of pages | 11 |
| Journal | Biosensors and Bioelectronics |
| Volume | 102 |
| DOIs | |
| Publication status | Published - 2018 Apr 15 |
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Keywords
- Circulating cancer stem cells
- Circulating tumor cells
- Metastasis
- Raman imaging
- Raman-active nanoprobes
ASJC Scopus subject areas
- Biotechnology
- Biophysics
- Biomedical Engineering
- Electrochemistry
Cite this
Selective isolation and noninvasive analysis of circulating cancer stem cells through Raman imaging. / Cho, Hyeon Yeol; Hossain, Md Khaled; Lee, Jin Ho; Han, Jiyou; Lee, Hun Joo; Kim, Kyeong Jun; Kim, Jong-Hoon; Lee, Ki Bum; Choi, Jeong Woo.
In: Biosensors and Bioelectronics, Vol. 102, 15.04.2018, p. 372-382.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Selective isolation and noninvasive analysis of circulating cancer stem cells through Raman imaging
AU - Cho, Hyeon Yeol
AU - Hossain, Md Khaled
AU - Lee, Jin Ho
AU - Han, Jiyou
AU - Lee, Hun Joo
AU - Kim, Kyeong Jun
AU - Kim, Jong-Hoon
AU - Lee, Ki Bum
AU - Choi, Jeong Woo
PY - 2018/4/15
Y1 - 2018/4/15
N2 - Circulating cancer stem cells (CCSCs), a rare circulating tumor cell (CTC) type, recently arose as a useful resource for monitoring and characterizing both cancers and their metastatic derivatives. However, due to the scarcity of CCSCs among hematologic cells in the blood and the complexity of the phenotype confirmation process, CCSC research can be extremely challenging. Hence, we report a nanoparticle-mediated Raman imaging method for CCSC characterization which profiles CCSCs based on their surface marker expression phenotypes. We have developed an integrated combinatorial Raman-Active Nanoprobe (RAN) system combined with a microfluidic chip to successfully process complete blood samples. CCSCs and CTCs were detected (90% efficiency) and classified in accordance with their respective surface marker expression via completely distinct Raman signals of RANs. Selectively isolated CCSCs (93% accuracy) were employed for both in vitro and in vivo tumor phenotyping to identify the tumorigenicity of the CCSCs. We utilized our new method to predict metastasis by screening blood samples from xenograft models, showing that upon CCSC detection, all subjects exhibited liver metastasis. Having highly efficient detection and noninvasive isolation capabilities, we have demonstrated that our RAN-based Raman imaging method will be valuable for predicting cancer metastasis and relapse via CCSC detection. Moreover, the exclusion of peak overlapping in CCSC analysis with our Raman imaging method will allow to expand the RAN families for various cancer types, therefore, increasing therapeutic efficacy by providing detailed molecular features of tumor subtypes.
AB - Circulating cancer stem cells (CCSCs), a rare circulating tumor cell (CTC) type, recently arose as a useful resource for monitoring and characterizing both cancers and their metastatic derivatives. However, due to the scarcity of CCSCs among hematologic cells in the blood and the complexity of the phenotype confirmation process, CCSC research can be extremely challenging. Hence, we report a nanoparticle-mediated Raman imaging method for CCSC characterization which profiles CCSCs based on their surface marker expression phenotypes. We have developed an integrated combinatorial Raman-Active Nanoprobe (RAN) system combined with a microfluidic chip to successfully process complete blood samples. CCSCs and CTCs were detected (90% efficiency) and classified in accordance with their respective surface marker expression via completely distinct Raman signals of RANs. Selectively isolated CCSCs (93% accuracy) were employed for both in vitro and in vivo tumor phenotyping to identify the tumorigenicity of the CCSCs. We utilized our new method to predict metastasis by screening blood samples from xenograft models, showing that upon CCSC detection, all subjects exhibited liver metastasis. Having highly efficient detection and noninvasive isolation capabilities, we have demonstrated that our RAN-based Raman imaging method will be valuable for predicting cancer metastasis and relapse via CCSC detection. Moreover, the exclusion of peak overlapping in CCSC analysis with our Raman imaging method will allow to expand the RAN families for various cancer types, therefore, increasing therapeutic efficacy by providing detailed molecular features of tumor subtypes.
KW - Circulating cancer stem cells
KW - Circulating tumor cells
KW - Metastasis
KW - Raman imaging
KW - Raman-active nanoprobes
UR - http://www.scopus.com/inward/record.url?scp=85034787952&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85034787952&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2017.11.049
DO - 10.1016/j.bios.2017.11.049
M3 - Article
C2 - 29174970
AN - SCOPUS:85034787952
VL - 102
SP - 372
EP - 382
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
SN - 0956-5663
ER -