Selective production of epothilone B by heterologous expression of propionyl-CoA synthetase in Sorangium cellulosum

Se Jong Han, Sang Woo Park, Byung Woo Kim, Sang Jun Sim

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The metabolic engineering of epothilones, as secondary metabolites, was investigated using Sorangium cellulosum to achieve the selective production of epothilone B, a potent anticancer agent. Thus, the propionyl-CoA synthetase gene (prpE) from Ralstonia solanacearum was heterologously expressed in S. cellulosum to increase the production of epothilone B. Propionyl-CoA synthetase converts propionate into propionyl-CoA, a potent precursor of epothilone B. The recombinant S. cellulosum containing the prpE gene exhibited a significant increase in the resolution of epothilones B/A, with an epothilone B to A ratio of 127 to 1, which was 100 times higher than that of the wild-type cells, demonstratin its potential use for the selective production of epothilone B.

Original languageEnglish
Pages (from-to)135-137
Number of pages3
JournalJournal of microbiology and biotechnology
Volume18
Issue number1
Publication statusPublished - 2008 Jan 28

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Keywords

  • Epothilone
  • Heterologous expression
  • Metabolic engineering
  • Propionyl-CoA synthetase
  • Sorangium cellulosum

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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