Selenite-induced apoptosis of osteoclasts mediated by the mitochondrial pathway

Youn Wook Chung, Tae Soo Kim, Soo Young Lee, Seoung Hoon Lee, Yongwon Choi, Nacksung Kim, Byung Moo Min, Dae Won Jeong, Ick Young Kim

Research output: Contribution to journalArticle

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Abstract

The possible effects of sodium selenite on mature osteoclasts were investigated. Incubation of osteoclast-like cells differentiated from RAW 264.7 cells with sodium selenite induced apoptosis as revealed by morphological changes, internucleosomal DNA fragmentation, and activation of caspase-3. Selenite also induced generation of the superoxide anion and reduced the number of free thiol groups in the osteoclast-like cells, suggestive of a shift to a more oxidizing intracellular environment. In addition, selenite induced protein aggregation by thiol cross-linking, loss of the mitochondrial membrane potential, and cytochrome c release in mitochondria isolated from the osteoclast-like cells. Finally, selenite-induced DNA fragmentation in osteoclasts was inhibited both by cyclosporin A, a blocker of the mitochondrial permeability transition pore, and by DEVD-CHO, a cell-permeable inhibitor of caspase-3. These results thus suggest that selenite induces apoptosis mediated by the mitochondrial pathway in mature osteoclasts.

Original languageEnglish
Pages (from-to)143-150
Number of pages8
JournalToxicology Letters
Volume160
Issue number2
DOIs
Publication statusPublished - 2006 Jan 5

Fingerprint

Selenious Acid
Osteoclasts
Apoptosis
Sodium Selenite
DNA Fragmentation
Sulfhydryl Compounds
Caspase 3
Mitochondria
CHO Cells
Mitochondrial Membrane Potential
DNA
Cytochromes c
Superoxides
Cyclosporine
Agglomeration
Chemical activation
Cells
Membranes
Proteins

Keywords

  • Apoptosis
  • Mitochondria
  • Osteoclast
  • Selenium

ASJC Scopus subject areas

  • Toxicology

Cite this

Selenite-induced apoptosis of osteoclasts mediated by the mitochondrial pathway. / Chung, Youn Wook; Kim, Tae Soo; Lee, Soo Young; Lee, Seoung Hoon; Choi, Yongwon; Kim, Nacksung; Min, Byung Moo; Jeong, Dae Won; Kim, Ick Young.

In: Toxicology Letters, Vol. 160, No. 2, 05.01.2006, p. 143-150.

Research output: Contribution to journalArticle

Chung, YW, Kim, TS, Lee, SY, Lee, SH, Choi, Y, Kim, N, Min, BM, Jeong, DW & Kim, IY 2006, 'Selenite-induced apoptosis of osteoclasts mediated by the mitochondrial pathway', Toxicology Letters, vol. 160, no. 2, pp. 143-150. https://doi.org/10.1016/j.toxlet.2005.06.019
Chung, Youn Wook ; Kim, Tae Soo ; Lee, Soo Young ; Lee, Seoung Hoon ; Choi, Yongwon ; Kim, Nacksung ; Min, Byung Moo ; Jeong, Dae Won ; Kim, Ick Young. / Selenite-induced apoptosis of osteoclasts mediated by the mitochondrial pathway. In: Toxicology Letters. 2006 ; Vol. 160, No. 2. pp. 143-150.
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