Selenium utilization in thioredoxin and catalytic advantage provided by selenocysteine

Moon Jung Kim, Byung Cheon Lee, Kwang Yeon Hwang, Vadim N. Gladyshev, Hwa Young Kim

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Thioredoxin (Trx) is a major thiol-disulfide reductase that plays a role in many biological processes, including DNA replication and redox signaling. Although selenocysteine (Sec)-containing Trxs have been identified in certain bacteria, their enzymatic properties have not been characterized. In this study, we expressed a selenoprotein Trx from Treponema denticola, an oral spirochete, in Escherichia coli and characterized this selenoenzyme and its natural cysteine (Cys) homologue using E. coli Trx1 as a positive control. <sup>75</sup>Se metabolic labeling and mutation analyses showed that the SECIS (Sec insertion sequence) of T. denticola selenoprotein Trx is functional in the E. coli Sec insertion system with specific selenium incorporation into the Sec residue. The selenoprotein Trx exhibited approximately 10-fold higher catalytic activity than the Sec-to-Cys version and natural Cys homologue and E. coli Trx1, suggesting that Sec confers higher catalytic activity on this thiol-disulfide reductase. Kinetic analysis also showed that the selenoprotein Trx had a 30-fold higher K<inf>m</inf> than Cys-containing homologues, suggesting that this selenoenzyme is adapted to work efficiently with high concentrations of substrate. Collectively, the results of this study support the hypothesis that selenium utilization in oxidoreductase systems is primarily due to the catalytic advantage provided by the rare amino acid, Sec.

Original languageEnglish
Pages (from-to)648-652
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume461
Issue number4
DOIs
Publication statusPublished - 2015 Jun 12

Fingerprint

Selenocysteine
Thioredoxins
Selenium
Selenoproteins
Escherichia coli
Cysteine
Treponema denticola
Oxidoreductases
Sulfhydryl Compounds
Disulfides
Catalyst activity
Biological Phenomena
Spirochaetales
Insertional Mutagenesis
DNA Replication
Labeling
Oxidation-Reduction
Bacteria
Amino Acids
Mutation

Keywords

  • Disulfide reductase
  • Selenoprotein
  • Thioredoxin
  • Treponema denticola

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Selenium utilization in thioredoxin and catalytic advantage provided by selenocysteine. / Kim, Moon Jung; Lee, Byung Cheon; Hwang, Kwang Yeon; Gladyshev, Vadim N.; Kim, Hwa Young.

In: Biochemical and Biophysical Research Communications, Vol. 461, No. 4, 12.06.2015, p. 648-652.

Research output: Contribution to journalArticle

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