Selenoprotein W enhances skeletal muscle differentiation by inhibiting TAZ binding to 14-3-3 protein

Yeong Ha Jeon, Yong Hwan Park, Jea Hwang Lee, Jeong Ho Hong, Ick Young Kim

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Selenoprotein W (SelW) is expressed in various tissues, particularly in skeletal muscle. We have previously reported that SelW is up-regulated during C2C12 skeletal muscle differentiation and inhibits binding of 14-3-3 to its target proteins. 14-3-3 reduces myogenic differentiation by inhibiting nuclear translocation of transcriptional co-activator with PDZ-binding motif (TAZ). Phosphorylation of TAZ at Ser89 is required for binding to 14-3-3, leading to cytoplasmic retention of TAZ and a delay in myogenic differentiation. Here, we show that myogenic differentiation was delayed in SelW-knockdown C2C12 cells. Down-regulation of SelW also increased TAZ binding to 14-3-3, which eventually resulted in decreasing translocation of TAZ to the nucleus. However, phosphorylation of TAZ at Ser89 was not affected. Although phosphorylation of TAZ at Ser89 was sustained by the phosphatase inhibitor okadaic acid, nuclear translocation of TAZ was increased by ectopic expression of SelW. This result was due to decreased binding of TAZ to 14-3-3. We also found that the interaction between TAZ and MyoD was increased by ectopic expression of SelW. Taken together, these findings strongly demonstrate that SelW enhances C2C12 cell differentiation by inhibiting TAZ binding to 14-3-3.

Original languageEnglish
Pages (from-to)1356-1364
Number of pages9
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1843
Issue number7
DOIs
Publication statusPublished - 2014 Jul

Keywords

  • 14-3-3
  • Selenoprotein W
  • TAZ

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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