Selenoprotein W is a glutathione-dependent antioxidant in vivo

Dae won Jeong, Tae Soo Kim, Youn Wook Chung, Byeong Jae Lee, Ick Young Kim

Research output: Contribution to journalArticlepeer-review

128 Citations (Scopus)

Abstract

The function of selenoprotein W (Se-W) was investigated by cloning the corresponding cDNA from mouse brain and expressing it in CHO cells and H1299 human lung cancer cells. Overexpression of Se-W markedly reduced the sensitivity of both cell lines to H2O2 cytotoxicity. The intracellular peroxide concentration of the transfected cells was lower than that of the parental cells in the absence or presence of extracellular H2O2. The resistance to oxidative stress conferred by Se-W was dependent on glutathione. Expression of Se-W mutants in which selenocysteine-13 or cysteine-37 was replaced by serine did not confer resistance to H2O2, implicating these residues in the antioxidant activity of Se-W in vivo.

Original languageEnglish
Pages (from-to)225-228
Number of pages4
JournalFEBS Letters
Volume517
Issue number1-3
DOIs
Publication statusPublished - 2002 Apr 24

Keywords

  • Antioxidant
  • Glutathione
  • Hydrogen peroxide
  • Selenocysteine
  • Selenoprotein W

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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