Selenoprotein W promotes cell cycle recovery from G2 arrest through the activation of CDC25B

Yong Hwan Park, Yeong Ha Jeon, Ick Young Kim

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Selenoprotein W (SelW) contains a highly reactive selenocysteine (Sec; U) in the CXXU motif corresponding to the CXXC motif in thioredoxin (Trx) and thus it appears to be involved in regulating the cellular redox state. Recent reports on the interaction between SelW and 14-3-3 suggest that SelW may be redox dependently involved in the cell cycle. However, the precise function of SelW has not yet been elucidated. Here, we show that SelW is involved in the G2-M transition, especially in the recovery from G2 arrest after deoxyribonucleic acid (DNA) damage. Knockdown of SelW significantly accumulated phosphorylated cyclin-dependent kinase (Cdk1), which eventually led to a delay in recovery from G2 arrest. We also found that inactive Cdk1 is caused by the sustained inactivation of CDC25B, which removes the inhibitory phosphate from Cdk1. Our observation from this study reveals that SelW activated CDC25B by promoting the dissociation of 14-3-3 from CDC25B through the reduction of the intramolecular disulfide bond during recovery. We suggest that SelW plays an important role in the recovery from G2 arrest by determining the dissociation of 14-3-3 from CDC25B in a redox-dependent manner.

Original languageEnglish
Pages (from-to)2217-2226
Number of pages10
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1823
Issue number12
DOIs
Publication statusPublished - 2012 Dec

Keywords

  • 14-3-3
  • CDC25B
  • Cdk1
  • Cell cycle
  • G2 arrest
  • Selenoprotein W

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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