Self-assembled nanoparticles containing hydrophobically modified glycol chitosan for gene delivery

Hyuk Sang Yoo, Jung Eun Lee, Hesson Chung, Ick Chan Kwon, Seo Young Jeong

Research output: Contribution to journalArticle

177 Citations (Scopus)

Abstract

A self-assembled nanoparticle was prepared using a hydrophobically modified glycol chitosan for gene delivery. A primary amine of glycol chitosan was modified with 5β-cholanic acid to prepare a hydrophobically modified glycol chitosan (HGC). The modified chitosan spontaneously formed DNA nanoparticles by a hydrophobic interaction between HGC and hydrophobized DNA. As the HGC content increased, the encapsulation efficiencies of DNA increased while the size of HGC nanoparticles decreased. Upon increasing HGC contents, HGC nanoparticle became less cytotoxic. The increased HGC contents also facilitated endocytic uptakes of HGC nanoparticles by COS-1 cells, which were confirmed by a confocal microscopy. The HGC nanoparticles showed increasing in vitro transfection efficiencies in the presence serum. In vivo results also showed that the HGC nanoparticles had superior transfection efficiencies to naked DNA and a commercialized transfection agent. The HGC nanoparticles composed of hydrophobized DNA and hydrophobically modified glycol chitosan played a significant role in enhancing transfection efficiencies in vitro as well as in vivo.

Original languageEnglish
Pages (from-to)235-243
Number of pages9
JournalJournal of Controlled Release
Volume103
Issue number1
DOIs
Publication statusPublished - 2005 Mar 2

Keywords

  • Chitosan
  • Gene delivery
  • In vivo transfection
  • Self-assembled

ASJC Scopus subject areas

  • Pharmaceutical Science

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