Self-assembled nanoparticles containing hydrophobically modified glycol chitosan for gene delivery

Hyuk Sang Yoo, Jung Eun Lee, Hesson Chung, Ick Chan Kwon, Seo Young Jeong

Research output: Contribution to journalArticle

165 Citations (Scopus)

Abstract

A self-assembled nanoparticle was prepared using a hydrophobically modified glycol chitosan for gene delivery. A primary amine of glycol chitosan was modified with 5β-cholanic acid to prepare a hydrophobically modified glycol chitosan (HGC). The modified chitosan spontaneously formed DNA nanoparticles by a hydrophobic interaction between HGC and hydrophobized DNA. As the HGC content increased, the encapsulation efficiencies of DNA increased while the size of HGC nanoparticles decreased. Upon increasing HGC contents, HGC nanoparticle became less cytotoxic. The increased HGC contents also facilitated endocytic uptakes of HGC nanoparticles by COS-1 cells, which were confirmed by a confocal microscopy. The HGC nanoparticles showed increasing in vitro transfection efficiencies in the presence serum. In vivo results also showed that the HGC nanoparticles had superior transfection efficiencies to naked DNA and a commercialized transfection agent. The HGC nanoparticles composed of hydrophobized DNA and hydrophobically modified glycol chitosan played a significant role in enhancing transfection efficiencies in vitro as well as in vivo.

Original languageEnglish
Pages (from-to)235-243
Number of pages9
JournalJournal of Controlled Release
Volume103
Issue number1
DOIs
Publication statusPublished - 2005 Mar 2
Externally publishedYes

Fingerprint

Nanoparticles
Genes
Transfection
DNA
glycol-chitosan
COS Cells
Chitosan
Hydrophobic and Hydrophilic Interactions
Confocal Microscopy
Amines

Keywords

  • Chitosan
  • Gene delivery
  • In vivo transfection
  • Self-assembled

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Self-assembled nanoparticles containing hydrophobically modified glycol chitosan for gene delivery. / Yoo, Hyuk Sang; Lee, Jung Eun; Chung, Hesson; Kwon, Ick Chan; Jeong, Seo Young.

In: Journal of Controlled Release, Vol. 103, No. 1, 02.03.2005, p. 235-243.

Research output: Contribution to journalArticle

Yoo, Hyuk Sang ; Lee, Jung Eun ; Chung, Hesson ; Kwon, Ick Chan ; Jeong, Seo Young. / Self-assembled nanoparticles containing hydrophobically modified glycol chitosan for gene delivery. In: Journal of Controlled Release. 2005 ; Vol. 103, No. 1. pp. 235-243.
@article{328be5e08342498ebd1102eb799273cf,
title = "Self-assembled nanoparticles containing hydrophobically modified glycol chitosan for gene delivery",
abstract = "A self-assembled nanoparticle was prepared using a hydrophobically modified glycol chitosan for gene delivery. A primary amine of glycol chitosan was modified with 5β-cholanic acid to prepare a hydrophobically modified glycol chitosan (HGC). The modified chitosan spontaneously formed DNA nanoparticles by a hydrophobic interaction between HGC and hydrophobized DNA. As the HGC content increased, the encapsulation efficiencies of DNA increased while the size of HGC nanoparticles decreased. Upon increasing HGC contents, HGC nanoparticle became less cytotoxic. The increased HGC contents also facilitated endocytic uptakes of HGC nanoparticles by COS-1 cells, which were confirmed by a confocal microscopy. The HGC nanoparticles showed increasing in vitro transfection efficiencies in the presence serum. In vivo results also showed that the HGC nanoparticles had superior transfection efficiencies to naked DNA and a commercialized transfection agent. The HGC nanoparticles composed of hydrophobized DNA and hydrophobically modified glycol chitosan played a significant role in enhancing transfection efficiencies in vitro as well as in vivo.",
keywords = "Chitosan, Gene delivery, In vivo transfection, Self-assembled",
author = "Yoo, {Hyuk Sang} and Lee, {Jung Eun} and Hesson Chung and Kwon, {Ick Chan} and Jeong, {Seo Young}",
year = "2005",
month = "3",
day = "2",
doi = "10.1016/j.jconrel.2004.11.033",
language = "English",
volume = "103",
pages = "235--243",
journal = "Journal of Controlled Release",
issn = "0168-3659",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Self-assembled nanoparticles containing hydrophobically modified glycol chitosan for gene delivery

AU - Yoo, Hyuk Sang

AU - Lee, Jung Eun

AU - Chung, Hesson

AU - Kwon, Ick Chan

AU - Jeong, Seo Young

PY - 2005/3/2

Y1 - 2005/3/2

N2 - A self-assembled nanoparticle was prepared using a hydrophobically modified glycol chitosan for gene delivery. A primary amine of glycol chitosan was modified with 5β-cholanic acid to prepare a hydrophobically modified glycol chitosan (HGC). The modified chitosan spontaneously formed DNA nanoparticles by a hydrophobic interaction between HGC and hydrophobized DNA. As the HGC content increased, the encapsulation efficiencies of DNA increased while the size of HGC nanoparticles decreased. Upon increasing HGC contents, HGC nanoparticle became less cytotoxic. The increased HGC contents also facilitated endocytic uptakes of HGC nanoparticles by COS-1 cells, which were confirmed by a confocal microscopy. The HGC nanoparticles showed increasing in vitro transfection efficiencies in the presence serum. In vivo results also showed that the HGC nanoparticles had superior transfection efficiencies to naked DNA and a commercialized transfection agent. The HGC nanoparticles composed of hydrophobized DNA and hydrophobically modified glycol chitosan played a significant role in enhancing transfection efficiencies in vitro as well as in vivo.

AB - A self-assembled nanoparticle was prepared using a hydrophobically modified glycol chitosan for gene delivery. A primary amine of glycol chitosan was modified with 5β-cholanic acid to prepare a hydrophobically modified glycol chitosan (HGC). The modified chitosan spontaneously formed DNA nanoparticles by a hydrophobic interaction between HGC and hydrophobized DNA. As the HGC content increased, the encapsulation efficiencies of DNA increased while the size of HGC nanoparticles decreased. Upon increasing HGC contents, HGC nanoparticle became less cytotoxic. The increased HGC contents also facilitated endocytic uptakes of HGC nanoparticles by COS-1 cells, which were confirmed by a confocal microscopy. The HGC nanoparticles showed increasing in vitro transfection efficiencies in the presence serum. In vivo results also showed that the HGC nanoparticles had superior transfection efficiencies to naked DNA and a commercialized transfection agent. The HGC nanoparticles composed of hydrophobized DNA and hydrophobically modified glycol chitosan played a significant role in enhancing transfection efficiencies in vitro as well as in vivo.

KW - Chitosan

KW - Gene delivery

KW - In vivo transfection

KW - Self-assembled

UR - http://www.scopus.com/inward/record.url?scp=13844265668&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=13844265668&partnerID=8YFLogxK

U2 - 10.1016/j.jconrel.2004.11.033

DO - 10.1016/j.jconrel.2004.11.033

M3 - Article

VL - 103

SP - 235

EP - 243

JO - Journal of Controlled Release

JF - Journal of Controlled Release

SN - 0168-3659

IS - 1

ER -