Self-assembly of protein nanoarrays on block copolymer templates

K. H.Aaron Lau, Joona Bang, Dong Ha Kim, Wolfgang Knoll

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

There is considerable interest in developing functional protein arrays on the nanoscale for high-throughput protein-based array technology, and for the study of biomolecular and cell interactions at the physical scale of the biomolecules. To these ends, self-assembly based techniques may be desirable for the nanopatterning of proteins on large sample areas without the use of lithography equipment. We present a fast, general approach for patterning proteins (and potentially other biomolecules) on the nanoscale, which takes advantage of the ability of block copolymers to self-assemble into ordered surface nanopatterns with defined chemical heterogeneity. We demonstrate nanoarrays of immunoglobulin and bovine serum albumin on polystyreneblock -poly(methyl methacrylate) templates, and illustrate the applicability of our technique through immunoassays and DNA sensing performed on the protein nanoarrays. Furthermore, we show that the pattern formation mechanism is a nanoscale effect originating from a combination of fluid flow forces and geometric restrictions templated by an underlying nanopattern with a difference in protein adsorption behavior on adjacent, chemically distinct surfaces. This understanding may provide a framework for extending the patterning approach to other proteins and material systems.

Original languageEnglish
Pages (from-to)3148-3157
Number of pages10
JournalAdvanced Functional Materials
Volume18
Issue number20
DOIs
Publication statusPublished - 2008 Oct 23

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics

Fingerprint Dive into the research topics of 'Self-assembly of protein nanoarrays on block copolymer templates'. Together they form a unique fingerprint.

  • Cite this