Sensitive and selective analysis of a wide concentration range of IGFBP7 using a surface plasmon resonance biosensor

Dae Ho Jang, Youngbo Choi, Yong Soo Choi, Sun Mi Kim, Hojung Kwak, Sehyun Shin, Surin Hong

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A sensitive method for selectively detecting insulin-like growth factor-binding protein 7 (IGFBP7) over a wide range of concentrations based on the surface plasmon resonance (SPR) biosensing techniques is described. IGFBP7 has been shown to regulate cell proliferation, cell adhesion, cellular senescence, apoptosis, and angiogenesis in several different cancer cell lines. Since the concentration of IGFBP7 can vary widely in the body, determining the precise concentration of IGFBP7 over a wide range of concentrations is important, since it serves as an inducible biomarker for both disease diagnosis and subsequent therapy. The SPR sensing method is based on the selective interaction of IGFBP7 with specific anti-IGFBP7 proteins on a gold thin film, which was covalently bound to the Fc-binding domain of protein G on a mixed self-assembled monolayer composed of DSNHS (S2(CH2)11COO(CH2)2COO-(N-hydroxysuccinimide)) and mercaptoundecanol, and effect of this on changes in the SPR profiles. The limit of detection (LOD) of the SPR biosensor was determined to be 10ng/ml, which is a reasonable LOD value for biomedical applications. The response is essentially linear in the concentration range of 10-300ng/ml. The SPR biosensor also shows specificity for IGFBP7 compared to that for biologically relevant interleukin (IL) derivatives including IL4, IL23, IL29, and IFG1. These molecules are also present along with IGFBP7 in the cell culture medium and have the potential to interfere with the analysis. Finally, the level secretion of IGFBP7 from cancer cells detected by the SPR biosensor showed a good correlation with a commercial kit using an IGFBP7 enzyme-linked immunosorbent assay. The findings reported herein indicate that the SPR biosensor for IGFBP7 would be applicable in a wide variety of biomedical fields.

Original languageEnglish
Pages (from-to)887-891
Number of pages5
JournalColloids and Surfaces B: Biointerfaces
Volume123
DOIs
Publication statusPublished - 2014 Nov 1

ASJC Scopus subject areas

  • Biotechnology
  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Surfaces and Interfaces

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