Sensitive detection of adipokines for early diagnosis of type 2 diabetes using enzyme-linked antibody-aptamer sandwich (ELAAS) assays

Su Jin Lee; Jee Woong Park; In Ae Kim; Byung Soo Youn; Man Bock Gu

doi:10.1016/j.snb.2012.04.016

Sensitive detection of adipokines for early diagnosis of type 2 diabetes using enzyme-linked antibody-aptamer sandwich (ELAAS) assays

Su Jin Lee, Jee Woong Park, In Ae Kim, Byung Soo Youn, Man Bock Gu

Department of Biotechnology

Research output: Contribution to journal › Article

15 Citations (Scopus)

Abstract

We developed an enzyme-linked antibody-ssDNA aptamer sandwich (ELAAS) method to detect three adipokines for diagnosing type 2 diabetes. For this application, three different aptamers for retinol-binding protein-4 (RBP4), visceral adipose tissue-derived serpin (Vaspin), and nicotinamide phosphoribosyltransferase (Nampt/visfatin) were used as capturing probes, and enzyme-linked polyclonal antibodies were used for signal generation. The sensitivities of this method for all three proteins were enhanced by at least 20-fold to up to 68-fold higher than that of the surface plasmon resonance (SPR) system, which only uses aptamers as capturing probes. In addition, this assay method exhibited equal sensitivity in the detection of each adipokine spiked in diluted human serum. In particular, the detection limit for RBP4 was similar to that obtained by western blotting, as reported previously. The simplicity, reproducibility, and high sensitivity of the ELAAS method could allow it to be extensively utilized in field.

Original language	English
Pages (from-to)	243-248
Number of pages	6
Journal	Sensors and Actuators, B: Chemical
Volume	168
DOIs	https://doi.org/10.1016/j.snb.2012.04.016
Publication status	Published - 2012 Jun 20

Keywords

Adipokines
Antibodies
Aptamers
ELAAS biosensor
Type 2 diabetes

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

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Lee, S. J., Park, J. W., Kim, I. A., Youn, B. S., & Gu, M. B. (2012). Sensitive detection of adipokines for early diagnosis of type 2 diabetes using enzyme-linked antibody-aptamer sandwich (ELAAS) assays. Sensors and Actuators, B: Chemical, 168, 243-248. https://doi.org/10.1016/j.snb.2012.04.016

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abstract = "We developed an enzyme-linked antibody-ssDNA aptamer sandwich (ELAAS) method to detect three adipokines for diagnosing type 2 diabetes. For this application, three different aptamers for retinol-binding protein-4 (RBP4), visceral adipose tissue-derived serpin (Vaspin), and nicotinamide phosphoribosyltransferase (Nampt/visfatin) were used as capturing probes, and enzyme-linked polyclonal antibodies were used for signal generation. The sensitivities of this method for all three proteins were enhanced by at least 20-fold to up to 68-fold higher than that of the surface plasmon resonance (SPR) system, which only uses aptamers as capturing probes. In addition, this assay method exhibited equal sensitivity in the detection of each adipokine spiked in diluted human serum. In particular, the detection limit for RBP4 was similar to that obtained by western blotting, as reported previously. The simplicity, reproducibility, and high sensitivity of the ELAAS method could allow it to be extensively utilized in field.",

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AU - Gu, Man Bock

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