Separation of extracellular nanovesicles and apoptotic bodies from cancer cell culture broth using tunable microfluidic systems

Soojeong Shin, Daeyoung Han, Min Chul Park, Ji Young Mun, Jonghoon Choi, Honggu Chun, Sunghoon Kim, Jong Wook Hong

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Extracellular vesicles (EVs) are the cell-secreted nano- and micro-sized particles consisted of lipid bilayer containing nucleic acids and proteins for diagnosis and therapeutic applications. The inherent complexity of EVs is a source of heterogeneity in various potential applications of the biological nanovesicles including analysis. To diminish heterogeneity, EV should be isolated and separated according to their sizes and cargos. However, current technologies do not meet the requirements. We showed noninvasive and precise separation of EVs based on their sizes without any recognizable damages. We separated atto-liter volumes of biological nanoparticles through operation of the present system showing relatively large volume of sample treatment to milliliters within an hour. We observed distinct size and morphological differences of 30 to 100 nm of exosomes and apoptotic bodies through TEM analysis. Indeed, we confirmed the biological moiety variations through immunoblotting with noninvasively separated EVs opening new windows in study and application of the biological nanoparticles.

Original languageEnglish
Article number9907
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - 2017 Dec 1

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Cell culture
Microfluidics
Nanoparticles
Lipid bilayers
Nucleic Acids
Transmission electron microscopy
Proteins

ASJC Scopus subject areas

  • General

Cite this

Separation of extracellular nanovesicles and apoptotic bodies from cancer cell culture broth using tunable microfluidic systems. / Shin, Soojeong; Han, Daeyoung; Park, Min Chul; Mun, Ji Young; Choi, Jonghoon; Chun, Honggu; Kim, Sunghoon; Hong, Jong Wook.

In: Scientific Reports, Vol. 7, No. 1, 9907, 01.12.2017.

Research output: Contribution to journalArticle

Shin, Soojeong ; Han, Daeyoung ; Park, Min Chul ; Mun, Ji Young ; Choi, Jonghoon ; Chun, Honggu ; Kim, Sunghoon ; Hong, Jong Wook. / Separation of extracellular nanovesicles and apoptotic bodies from cancer cell culture broth using tunable microfluidic systems. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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