Separation of platelets from whole blood using standing surface acoustic waves in a microchannel

Jeonghun Nam, Hyunjung Lim, Dookon Kim, Sehyun Shin

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

Platelet separation from blood is essential for biochemical analyses and clinical diagnosis. In this article, we propose a method to separate platelets from undiluted whole blood using standing surface acoustic waves (SSAWs) in a microfluidic device. A polydimethylsiloxane (PDMS) microfluidic channel was fabricated and integrated with interdigitated transducer (IDT) electrodes patterned on a piezoelectric substrate. To avoid shear-induced activation of platelets, the blood sample flow was hydrodynamically focused by introducing sheath flow from two side-inlets and pressure nodes were designed to locate at side walls. By means of flow cytometric analysis, the RBC clearance ratio from whole blood was found to be over 99% and the purity of platelets was close to 98%. Conclusively, the present technique using SSAWs can directly separate platelets from undiluted whole blood with higher purity than other methods.

Original languageEnglish
Pages (from-to)3361-3364
Number of pages4
JournalLab on a Chip - Miniaturisation for Chemistry and Biology
Volume11
Issue number19
DOIs
Publication statusPublished - 2011 Oct 7

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Platelets
Microchannels
Surface waves
Blood
Blood Platelets
Acoustic waves
Lab-On-A-Chip Devices
Microfluidics
Platelet Activation
Polydimethylsiloxane
Transducers
Electrodes
Chemical activation
Pressure
Substrates

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)
  • Bioengineering
  • Biomedical Engineering

Cite this

Separation of platelets from whole blood using standing surface acoustic waves in a microchannel. / Nam, Jeonghun; Lim, Hyunjung; Kim, Dookon; Shin, Sehyun.

In: Lab on a Chip - Miniaturisation for Chemistry and Biology, Vol. 11, No. 19, 07.10.2011, p. 3361-3364.

Research output: Contribution to journalArticle

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