Separation of platelets from whole blood using standing surface acoustic waves in a microchannel

Jeonghun Nam, Hyunjung Lim, Dookon Kim, Sehyun Shin

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

Platelet separation from blood is essential for biochemical analyses and clinical diagnosis. In this article, we propose a method to separate platelets from undiluted whole blood using standing surface acoustic waves (SSAWs) in a microfluidic device. A polydimethylsiloxane (PDMS) microfluidic channel was fabricated and integrated with interdigitated transducer (IDT) electrodes patterned on a piezoelectric substrate. To avoid shear-induced activation of platelets, the blood sample flow was hydrodynamically focused by introducing sheath flow from two side-inlets and pressure nodes were designed to locate at side walls. By means of flow cytometric analysis, the RBC clearance ratio from whole blood was found to be over 99% and the purity of platelets was close to 98%. Conclusively, the present technique using SSAWs can directly separate platelets from undiluted whole blood with higher purity than other methods.

Original languageEnglish
Pages (from-to)3361-3364
Number of pages4
JournalLab on a Chip
Volume11
Issue number19
DOIs
Publication statusPublished - 2011 Oct 7

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Fingerprint Dive into the research topics of 'Separation of platelets from whole blood using standing surface acoustic waves in a microchannel'. Together they form a unique fingerprint.

  • Cite this