Separation of the antioxidant compound quercitrin from Lindera obtusiloba blume and its antimelanogenic effect on B16F10 melanoma cells

Chung Oui Hong, Hyun Ah Lee, Chae Hong Rhee, Se Young Choung, Kwang Won Lee

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Considering the growing evidence of the presence of antioxidant compounds in plant extracts, the objectives of this study were to identify antioxidant compounds in Lindera obtusiloba Blume (Lauraceae) and to evaluate their antimelanogenic activities on B16F10 melanoma cells. Organic solvent fractions were separated from L. obtusiloba extracts (LOE). The ethyl acetate fraction (LOE-E) was significantly active against oxidative damage induced by tert-butyl hydroperoxide in primary rat hepatocytes. Two single purified compounds, quercitrin (quercetin-3-O-α-L-rhamnopyranoside) and afzelin (kaempferol-3-O-α-L-rhamnoside), were identified by HPLC and NMR. These compounds were evaluated for antioxidant activities by 1,1-diphenyl 2-picrylhydrazyl (DPPH) radical scavenging assay and ferric reducing antioxidant power (FRAP) assay, and for their antimelanogenic activities by tyrosinase inhibitory assay melanin formation inhibition assay and Western bolt analysis for the signaling pathway. The significant effects of quercitrin on antioxidant and antimelanogenic activities, and signal modulation of ERK and MITF in B16F10 melanoma cells were observed. This is the first report to identify quercitrin in L. obtusiloba and its whitening effect.

Original languageEnglish
Pages (from-to)58-64
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Volume77
Issue number1
DOIs
Publication statusPublished - 2013 Feb 14

Keywords

  • Antimelanogenic activity
  • Antioxidant activity
  • Kaempferol- 3-O-α-L-rhamnoside (afzelin)
  • Lindera obtusilob
  • Quercetin-3-O-α- L-rhamnopyranoside (quercitrin)

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Analytical Chemistry
  • Organic Chemistry

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