Sequence analysis of hemagglutinin and nucleoprotein genes of measles viruses isolated in Korea during the 2000 epidemic

Byoung Kuk Na, Jin Soo Lee, Gu Choul Shin, Ju Mi Shin, Joo Yeon Lee, Jae Keun Chung, Dong Ryong Ha, Jong Koo Lee, Sang Hyuk Ma, Hae Wol Cho, Chun Kang, Woo Joo Kim

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Abstract

To characterize the genetic properties of currently circulating measles viruses in Korea, the complete nucleotide sequences of hemagglutinin (H) protein and nucleoprotein (N) genes of Korean viruses were analyzed. The entire genes of H and N were directly amplified by RT-PCR from each clinical specimen and sequenced. Sequence analyses of H and N genes indicated that all Korean viruses had a high degree of homology (> 99.8%) when compared with each other. The Korean viruses differed from other wild-type viruses by as much as 6.8% in the H gene and 6.5% in the N gene at the nucleotide level. The deduced amino acid variability was up to 6.4% for the H protein and up to 6.5% for the N protein. Phylogenetic analyses of nucleotide sequences and deduced amino acid sequences of the H and N genes revealed that all Korean viruses were grouped into the clade H1.

Original languageEnglish
Pages (from-to)143-149
Number of pages7
JournalVirus Research
Volume81
Issue number1-2
DOIs
Publication statusPublished - 2001 Dec 4

Fingerprint

Hemagglutinins
Korea
Sequence Analysis
Viruses
Genes
Measles virus
Proteins
Nucleoproteins
Measles virus nucleoprotein
Amino Acid Sequence
Nucleotides
Amino Acids
Polymerase Chain Reaction

Keywords

  • Hemagglutinin
  • Measles virus
  • Nucleoprotein
  • Sequence

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases
  • Cancer Research

Cite this

Sequence analysis of hemagglutinin and nucleoprotein genes of measles viruses isolated in Korea during the 2000 epidemic. / Na, Byoung Kuk; Lee, Jin Soo; Shin, Gu Choul; Mi Shin, Ju; Lee, Joo Yeon; Chung, Jae Keun; Ha, Dong Ryong; Lee, Jong Koo; Ma, Sang Hyuk; Cho, Hae Wol; Kang, Chun; Kim, Woo Joo.

In: Virus Research, Vol. 81, No. 1-2, 04.12.2001, p. 143-149.

Research output: Contribution to journalArticle

Na, BK, Lee, JS, Shin, GC, Mi Shin, J, Lee, JY, Chung, JK, Ha, DR, Lee, JK, Ma, SH, Cho, HW, Kang, C & Kim, WJ 2001, 'Sequence analysis of hemagglutinin and nucleoprotein genes of measles viruses isolated in Korea during the 2000 epidemic', Virus Research, vol. 81, no. 1-2, pp. 143-149. https://doi.org/10.1016/S0168-1702(01)00346-X
Na, Byoung Kuk ; Lee, Jin Soo ; Shin, Gu Choul ; Mi Shin, Ju ; Lee, Joo Yeon ; Chung, Jae Keun ; Ha, Dong Ryong ; Lee, Jong Koo ; Ma, Sang Hyuk ; Cho, Hae Wol ; Kang, Chun ; Kim, Woo Joo. / Sequence analysis of hemagglutinin and nucleoprotein genes of measles viruses isolated in Korea during the 2000 epidemic. In: Virus Research. 2001 ; Vol. 81, No. 1-2. pp. 143-149.
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