Sequence analysis of human T cell lymphotropic virus type I strains from southern India

Gene amplification and direct sequencing from whole blood blotted onto filter paper

V. R. Nerurkar, P. G. Babu, Ki-Joon Song, R. R. Melland, C. Gnanamuthu, N. K. Saraswathi, M. Chandy, M. S. Godec, T. J. John, R. Yanagihara

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Human T cell lymphotropic virus type I (HTLV-I) infection in India has been found to be associated with adult T cell leukaemia/lymphoma (ATLL) and HTLV-I-associated myelopathy/tropical spastic paraparesis (HAM/TSP) among life-long residents of southern India. To examine the heterogeneity of HTLV-I strains from southern India and to determine their relationship with the sequence variants of HTLV-I from Melanesia, 1149 nucleotides spanning selected regions of the HTLV-I gag, pol, env and pX genes were amplified and directly sequenced from DNA extracted from whole blood blotted onto filter paper and from peripheral blood mononuclear cells, obtained from one patient with HAM/TSP, two with ATLL and eight asymptomatic carriers from Andhra Pradesh, Kerala and Tamil Nadu. Sequence alignments and comparisons indicated that the 11 HTLV-I strains from southern India were 99.2% to 100% identical among themselves and 98.7% to 100% identical to the Japanese prototype HTLV-I ATK. The majority of base substitutions were transitions and silent. No frameshifts, insertions, deletions or possibly disease-specific base changes were found in the regions sequenced. The observed clustering of the Indian HTLV-I strains with those from Japan, as determined by the maximum parsimony method, suggested a common source of HTLV-I infection with subsequent parallel evolution. Amplification of DNA from blood specimens collected on filter paper may be useful for the study of other blood-borne pathogens.

Original languageEnglish
Pages (from-to)2799-2805
Number of pages7
JournalJournal of General Virology
Volume74
Issue number12
DOIs
Publication statusPublished - 1993 Jan 1
Externally publishedYes

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Gene Amplification
Sequence Analysis
India
Viruses
T-Lymphocytes
Tropical Spastic Paraparesis
Adult T Cell Leukemia Lymphoma
Virus Diseases
pX Genes
Melanesia
Blood-Borne Pathogens
pol Genes
gag Genes
env Genes
Sequence Alignment
Spinal Cord Diseases
DNA
Cluster Analysis
Blood Cells
Japan

ASJC Scopus subject areas

  • Virology

Cite this

Sequence analysis of human T cell lymphotropic virus type I strains from southern India : Gene amplification and direct sequencing from whole blood blotted onto filter paper. / Nerurkar, V. R.; Babu, P. G.; Song, Ki-Joon; Melland, R. R.; Gnanamuthu, C.; Saraswathi, N. K.; Chandy, M.; Godec, M. S.; John, T. J.; Yanagihara, R.

In: Journal of General Virology, Vol. 74, No. 12, 01.01.1993, p. 2799-2805.

Research output: Contribution to journalArticle

Nerurkar, V. R. ; Babu, P. G. ; Song, Ki-Joon ; Melland, R. R. ; Gnanamuthu, C. ; Saraswathi, N. K. ; Chandy, M. ; Godec, M. S. ; John, T. J. ; Yanagihara, R. / Sequence analysis of human T cell lymphotropic virus type I strains from southern India : Gene amplification and direct sequencing from whole blood blotted onto filter paper. In: Journal of General Virology. 1993 ; Vol. 74, No. 12. pp. 2799-2805.
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