Sequence polymorphisms in Pvs48/45 and Pvs47 gametocyte and gamete surface proteins in Plasmodium vivax isolated in Korea

Mi Kyung Woo, Kyeong Ah Kim, Juyeon Kim, Jun Seo Oh, Eun Taek Han, Seong Soo A An, Chae Seung Lim

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Nucleotide sequence analyses of the Pvs48/45 and Pvs47 genes were conducted in 46 malaria patients from the Republic of Korea (ROK) (n = 40) and returning travellers from India (n = 3) and Indonesia (n = 3). The domain structures, which were based on cysteine residue position and secondary protein structure, were similar between Plasmodium vivax (Pvs48/45 and Pvs47) and Plasmodium falciparum (Pfs48/45 and Pfs47). In comparison to the Sal-1 reference strain (Pvs48/45, PVX_083235 and Pvs47, PVX_083240), Korean isolates revealed seven polymorphisms (E35K, H211N, K250N, D335Y, A376T, I380T and K418R) in Pvs48/45. These isolates could be divided into five haplotypes with the two major types having frequencies of 47.5% and 20%, respectivelfy. In Pvs47, 10 polymorphisms (F22L, F24L, K27E, D31N, V230I, M233I, E240D, I262T, I273M and A373V) were found and they could be divided into four haplotypes with one major type having a frequency of 75%. The Pvs48/45 isolates from India showed a unique amino acid substitution site (K26R). Compared to the Sal-1 and ROK isolates, the Pvs47 isolates from travellers returning from India and Indonesia had amino acid substitutions (S57T and I262K). The current data may contribute to the development of the malaria transmission-blocking vaccine in future clinical trials.

Original languageEnglish
Pages (from-to)359-367
Number of pages9
JournalMemorias do Instituto Oswaldo Cruz
Volume108
Issue number3
DOIs
Publication statusPublished - 2013 May 1

Fingerprint

Plasmodium vivax
Korea
Germ Cells
India
Republic of Korea
Membrane Proteins
Indonesia
Amino Acid Substitution
Haplotypes
Malaria
Secondary Protein Structure
Plasmodium falciparum
Cysteine
Sequence Analysis
Vaccines
Clinical Trials
Genes

Keywords

  • Malaria vaccine
  • Plasmodium vivax
  • Polymorphism
  • Pvs47
  • Pvs48/45
  • Republic of Korea
  • Transmission blocking vaccine

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Sequence polymorphisms in Pvs48/45 and Pvs47 gametocyte and gamete surface proteins in Plasmodium vivax isolated in Korea. / Woo, Mi Kyung; Kim, Kyeong Ah; Kim, Juyeon; Oh, Jun Seo; Han, Eun Taek; An, Seong Soo A; Lim, Chae Seung.

In: Memorias do Instituto Oswaldo Cruz, Vol. 108, No. 3, 01.05.2013, p. 359-367.

Research output: Contribution to journalArticle

Woo, Mi Kyung ; Kim, Kyeong Ah ; Kim, Juyeon ; Oh, Jun Seo ; Han, Eun Taek ; An, Seong Soo A ; Lim, Chae Seung. / Sequence polymorphisms in Pvs48/45 and Pvs47 gametocyte and gamete surface proteins in Plasmodium vivax isolated in Korea. In: Memorias do Instituto Oswaldo Cruz. 2013 ; Vol. 108, No. 3. pp. 359-367.
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AU - Oh, Jun Seo

AU - Han, Eun Taek

AU - An, Seong Soo A

AU - Lim, Chae Seung

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KW - Transmission blocking vaccine

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