Sequence polymorphisms in Pvs48/45 and Pvs47 gametocyte and gamete surface proteins in Plasmodium vivax isolated in Korea

Mi Kyung Woo, Kyeong Ah Kim, Juyeon Kim, Jun Seo Oh, Eun Taek Han, Seong Soo A. An, Chae Seung Lim

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Nucleotide sequence analyses of the Pvs48/45 and Pvs47 genes were conducted in 46 malaria patients from the Republic of Korea (ROK) (n = 40) and returning travellers from India (n = 3) and Indonesia (n = 3). The domain structures, which were based on cysteine residue position and secondary protein structure, were similar between Plasmodium vivax (Pvs48/45 and Pvs47) and Plasmodium falciparum (Pfs48/45 and Pfs47). In comparison to the Sal-1 reference strain (Pvs48/45, PVX_083235 and Pvs47, PVX_083240), Korean isolates revealed seven polymorphisms (E35K, H211N, K250N, D335Y, A376T, I380T and K418R) in Pvs48/45. These isolates could be divided into five haplotypes with the two major types having frequencies of 47.5% and 20%, respectivelfy. In Pvs47, 10 polymorphisms (F22L, F24L, K27E, D31N, V230I, M233I, E240D, I262T, I273M and A373V) were found and they could be divided into four haplotypes with one major type having a frequency of 75%. The Pvs48/45 isolates from India showed a unique amino acid substitution site (K26R). Compared to the Sal-1 and ROK isolates, the Pvs47 isolates from travellers returning from India and Indonesia had amino acid substitutions (S57T and I262K). The current data may contribute to the development of the malaria transmission-blocking vaccine in future clinical trials.

Original languageEnglish
Pages (from-to)359-367
Number of pages9
JournalMemorias do Instituto Oswaldo Cruz
Volume108
Issue number3
DOIs
Publication statusPublished - 2013 May

Keywords

  • Malaria vaccine
  • Plasmodium vivax
  • Polymorphism
  • Pvs47
  • Pvs48/45
  • Republic of Korea
  • Transmission blocking vaccine

ASJC Scopus subject areas

  • Microbiology (medical)

Fingerprint Dive into the research topics of 'Sequence polymorphisms in Pvs48/45 and Pvs47 gametocyte and gamete surface proteins in Plasmodium vivax isolated in Korea'. Together they form a unique fingerprint.

  • Cite this