Sequence variations of hepatitis B virus promoter regions in persistently infected patients

Y. M. Ha-Lee, Jung Hwa Lee, H. Pyun, Y. Kim, Jeongwon Sohn, Yunjung Cho, Y. Kim

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The HBV in the sera of two chronic active hepatitis patients were analyzed for the promoter sequence heterogeneity. In most cases, the proportion of any particular clone in the total viral populations was less than 50%, showing high mutation rates. In contrast, promoter sequences of HBV from asymptomatic carriers revealed only a few point mutations with no deletions. HBV in chronic patient harbored variants with multiple mutations throughout promoters including 1762 (A-to-T), 1764 (G-to-A) double mutation in C promoter and deletions near CCAAT site in S promoter. Unlike other three promoter regions, C, pre-S1 and S, of HBV which revealed a high level of sequence heterogeneity, the X promoter region (from nt 985 to 1430) showed little sequence heterogeneity within a patient. However, the predominant viral clones in two patients were quite different from each other. In addition to mutations in promoter regions, a deletion mutation in the translation start codon was also found in pre-S1 gene. The results in this report indicate that the mutation rates are not the same in all four promoters and that one of the strategies for maintaining persistent infection could be through mutations in viral promoters which then impair the balance of viral gene expressions.

Original languageEnglish
Pages (from-to)279-292
Number of pages14
JournalArchives of Virology
Volume146
Issue number2
DOIs
Publication statusPublished - 2001 Apr 12

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Genetic Promoter Regions
Hepatitis B virus
Mutation
Mutation Rate
Clone Cells
Initiator Codon
Viral Genes
Sequence Deletion
Chronic Hepatitis
Point Mutation
Gene Expression
Infection
Serum
Population
Genes

ASJC Scopus subject areas

  • Virology

Cite this

Sequence variations of hepatitis B virus promoter regions in persistently infected patients. / Ha-Lee, Y. M.; Lee, Jung Hwa; Pyun, H.; Kim, Y.; Sohn, Jeongwon; Cho, Yunjung; Kim, Y.

In: Archives of Virology, Vol. 146, No. 2, 12.04.2001, p. 279-292.

Research output: Contribution to journalArticle

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