Sequences of the Cytochrome C Oxidase Subunit i (COI) gene are suitable for species identification of Korean calliphorinae flies of forensic importance (Diptera: Calliphoridae)

Seong Hwan Park, Yong Zhang, Huguo Piao, Dong Ha Yu, Hyun Ju Jeong, Ga Young Yoo, Tae Ho Jo, Juck Joon Hwang

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Calliphorinae fly species are important indicators of the postmortem interval especially during early spring and late fall in Korea. Although nucleotide sequences of various Calliphorinae fly species are available, there has been no research on the cytochrome c oxidase subunit I (COI) nucleotide sequences of Korean Calliphorinae flies. Here, we report the full-length sequences of the COI gene of four Calliphorinae fly species collected in Korea (five individuals of Calliphora vicina, five Calliphora lata, four Triceratopyga calliphoroides and three Aldrichina grahami). Each COI gene was amplified by polymerase chain reaction and directly sequenced and the resulting nucleotide sequences were aligned and analyzed by MEGA4 software. The results indicate that COI nucleotide sequences can be used to distinguish between these four species. Our phylogenetic result coincides with recent taxonomic views on the subfamily Calliphorinae in that the genera Aldrichina and Triceratopyga are nested within the genus Calliphora.

Original languageEnglish
Pages (from-to)1131-1134
Number of pages4
JournalJournal of Forensic Sciences
Volume54
Issue number5
DOIs
Publication statusPublished - 2009 Sep

Keywords

  • Calliphoridae
  • Calliphorinae
  • Cytochrome c oxidase subunit I
  • Diptera
  • Forensic entomology
  • Forensic science
  • Postmortem interval
  • Species identification

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Genetics

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