Serinicoccus marinus gen. nov., sp. nov., a novel actinomycete with L-ornithine and L-serine in the peptidoglycan

Hana Yi, Peter Schumann, Kyounghee Sohn, Jongsik Chun

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

A Gram-positive bacterial strain containing L-ornithine as the diagnostic diamino acid was isolated from a sea-water-sample from the East Sea, Korea. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain JC1078T represents a phyletic line within the suborder Micrococcineae of the order Actinomycetales, adjacent to the genus Ornithinimicrobium. The highest sequence similarity values to the isolate were observed against Ornithinimicrobium humiphilum (94.3%) and Kytococcus sedentarius (94.1%). The strain was strictly aerobic and moderately halophilic with optimal growth at 2-3 % (w/v) NaCl. Cells were non-motile, non-sporulating and coccoid-shaped. The cell wall contains L-ornithine, glutamic acid, alanine, glycine and serine. The major menaquinone was MK-8(H4). The predominant cellular fatty acids were of the iso- and anteiso-methyl-branched types. The polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol and an unknown glycolipid. The acyl type of the glycan chain of peptidoglycan is acetyl. The DNA G+C content was 72 mol%. The combination of physiological, biochemical and chemotaxonomical data clearly separated the marine isolate from other members of the suborder Micrococcineae. On the basis of polyphasic evidence, it is proposed to classify strain JC1078T in a novel genus and species, for which the name Serinicoccus marinus gen. nov., sp. nov. is proposed. The type strain is JC1078T (=IMSNU 14026T = KCTC, 9980T = DSM 15273T).

Original languageEnglish
Pages (from-to)1585-1589
Number of pages5
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume54
Issue number5
DOIs
Publication statusPublished - 2004 Sep 1
Externally publishedYes

Fingerprint

Ornithine
Peptidoglycan
Actinobacteria
ornithine
peptidoglycans
serine
Serine
Actinomycetales
Vitamin K 2
Phosphatidylglycerols
Cardiolipins
new species
acid
Glycolipids
Base Composition
Seawater
Korea
Phosphatidylinositols
rRNA Genes
Oceans and Seas

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Applied Microbiology and Biotechnology
  • Immunology
  • Microbiology

Cite this

Serinicoccus marinus gen. nov., sp. nov., a novel actinomycete with L-ornithine and L-serine in the peptidoglycan. / Yi, Hana; Schumann, Peter; Sohn, Kyounghee; Chun, Jongsik.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 54, No. 5, 01.09.2004, p. 1585-1589.

Research output: Contribution to journalArticle

@article{25a8ae0271ab4e4bbb83c0583b862867,
title = "Serinicoccus marinus gen. nov., sp. nov., a novel actinomycete with L-ornithine and L-serine in the peptidoglycan",
abstract = "A Gram-positive bacterial strain containing L-ornithine as the diagnostic diamino acid was isolated from a sea-water-sample from the East Sea, Korea. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain JC1078T represents a phyletic line within the suborder Micrococcineae of the order Actinomycetales, adjacent to the genus Ornithinimicrobium. The highest sequence similarity values to the isolate were observed against Ornithinimicrobium humiphilum (94.3{\%}) and Kytococcus sedentarius (94.1{\%}). The strain was strictly aerobic and moderately halophilic with optimal growth at 2-3 {\%} (w/v) NaCl. Cells were non-motile, non-sporulating and coccoid-shaped. The cell wall contains L-ornithine, glutamic acid, alanine, glycine and serine. The major menaquinone was MK-8(H4). The predominant cellular fatty acids were of the iso- and anteiso-methyl-branched types. The polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol and an unknown glycolipid. The acyl type of the glycan chain of peptidoglycan is acetyl. The DNA G+C content was 72 mol{\%}. The combination of physiological, biochemical and chemotaxonomical data clearly separated the marine isolate from other members of the suborder Micrococcineae. On the basis of polyphasic evidence, it is proposed to classify strain JC1078T in a novel genus and species, for which the name Serinicoccus marinus gen. nov., sp. nov. is proposed. The type strain is JC1078T (=IMSNU 14026T = KCTC, 9980T = DSM 15273T).",
author = "Hana Yi and Peter Schumann and Kyounghee Sohn and Jongsik Chun",
year = "2004",
month = "9",
day = "1",
doi = "10.1099/ijs.0.03036-0",
language = "English",
volume = "54",
pages = "1585--1589",
journal = "The BMJ",
issn = "0730-6512",
publisher = "Kluwer Academic Publishers",
number = "5",

}

TY - JOUR

T1 - Serinicoccus marinus gen. nov., sp. nov., a novel actinomycete with L-ornithine and L-serine in the peptidoglycan

AU - Yi, Hana

AU - Schumann, Peter

AU - Sohn, Kyounghee

AU - Chun, Jongsik

PY - 2004/9/1

Y1 - 2004/9/1

N2 - A Gram-positive bacterial strain containing L-ornithine as the diagnostic diamino acid was isolated from a sea-water-sample from the East Sea, Korea. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain JC1078T represents a phyletic line within the suborder Micrococcineae of the order Actinomycetales, adjacent to the genus Ornithinimicrobium. The highest sequence similarity values to the isolate were observed against Ornithinimicrobium humiphilum (94.3%) and Kytococcus sedentarius (94.1%). The strain was strictly aerobic and moderately halophilic with optimal growth at 2-3 % (w/v) NaCl. Cells were non-motile, non-sporulating and coccoid-shaped. The cell wall contains L-ornithine, glutamic acid, alanine, glycine and serine. The major menaquinone was MK-8(H4). The predominant cellular fatty acids were of the iso- and anteiso-methyl-branched types. The polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol and an unknown glycolipid. The acyl type of the glycan chain of peptidoglycan is acetyl. The DNA G+C content was 72 mol%. The combination of physiological, biochemical and chemotaxonomical data clearly separated the marine isolate from other members of the suborder Micrococcineae. On the basis of polyphasic evidence, it is proposed to classify strain JC1078T in a novel genus and species, for which the name Serinicoccus marinus gen. nov., sp. nov. is proposed. The type strain is JC1078T (=IMSNU 14026T = KCTC, 9980T = DSM 15273T).

AB - A Gram-positive bacterial strain containing L-ornithine as the diagnostic diamino acid was isolated from a sea-water-sample from the East Sea, Korea. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain JC1078T represents a phyletic line within the suborder Micrococcineae of the order Actinomycetales, adjacent to the genus Ornithinimicrobium. The highest sequence similarity values to the isolate were observed against Ornithinimicrobium humiphilum (94.3%) and Kytococcus sedentarius (94.1%). The strain was strictly aerobic and moderately halophilic with optimal growth at 2-3 % (w/v) NaCl. Cells were non-motile, non-sporulating and coccoid-shaped. The cell wall contains L-ornithine, glutamic acid, alanine, glycine and serine. The major menaquinone was MK-8(H4). The predominant cellular fatty acids were of the iso- and anteiso-methyl-branched types. The polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol and an unknown glycolipid. The acyl type of the glycan chain of peptidoglycan is acetyl. The DNA G+C content was 72 mol%. The combination of physiological, biochemical and chemotaxonomical data clearly separated the marine isolate from other members of the suborder Micrococcineae. On the basis of polyphasic evidence, it is proposed to classify strain JC1078T in a novel genus and species, for which the name Serinicoccus marinus gen. nov., sp. nov. is proposed. The type strain is JC1078T (=IMSNU 14026T = KCTC, 9980T = DSM 15273T).

UR - http://www.scopus.com/inward/record.url?scp=5444260534&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=5444260534&partnerID=8YFLogxK

U2 - 10.1099/ijs.0.03036-0

DO - 10.1099/ijs.0.03036-0

M3 - Article

VL - 54

SP - 1585

EP - 1589

JO - The BMJ

JF - The BMJ

SN - 0730-6512

IS - 5

ER -