Serological responses to a soluble recombinant chimeric Plasmodium vivax circumsporozoite protein in VK210 and VK247 population

Yang Cheng, Daisuke Ito, Jetsumon Sattabongkot, Chae Seung Lim, Deok Hoon Kong, Kwon Soo Ha, Bo Wang, Takafumi Tsuboi, Eun Taek Han

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background: Circumsporozoite protein (CSP) is essential for sporozoite formation and sporozoite invasion into human hepatocyte. Previously, a recombinant P. vivax CSP based on chimeric repeats (rPvCSP-c) representing two major alleles VK210 and VK247 within central region has been designed. Naturally acquired humoral immune responses study show that antigenicity of rPvCSP-c was much higher than that of native strain. However, the serologic reactivity of rPvCSP-c was still unclear in detail. Methods. In present study, recognition of rPvCSP-c in vivax malaria typed VK210 and VK247 alleles was assessed. VK210 typed and VK247 typed sera from adult residents reacted specifically with rPvCSP-c using protein array and immunoblot assay. Additionally, anti-rPvCSP-c serum recognized the fixed VK210 and VK247 sporozoites by immunofluorescence assay. Furthermore, statistic analysis was performed for correlational detection. Results: The rPvCSP-c reacted with both VK210 typed and VK247 typed P. vivax infected patient sera and anti-rPvCSP-c immune serum also reacted with VK210 and VK247 sporozoite parasites of P. vivax specifically. There was a positive correlation between increased antibody level, age of patients and also associated with pvcsp repeat number, although the level of responses did vary considerably in their reactivity to the rPvCSP-c from negative to very high level within each age group. Conclusions: These data confirmed the serologic reactivity of the novel rPvCSP-c in exposed both VK210 and VK247 populations. These results strongly suggested that this recombinant CSP was biologically active and potently immunogenic across major strains and raised the prospect that this protein could be used as serologic marker.

Original languageEnglish
Article number323
JournalMalaria Journal
Volume12
Issue number1
DOIs
Publication statusPublished - 2013 Sep 17

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Plasmodium vivax
Sporozoites
Population
Serum
Alleles
Vivax Malaria
Protein Array Analysis
Humoral Immunity
Recombinant Proteins
Fluorescent Antibody Technique
Immune Sera
Hepatocytes
Parasites
Proteins
Age Groups
Protozoan circumsporozoite protein
Antibodies

ASJC Scopus subject areas

  • Infectious Diseases
  • Parasitology

Cite this

Serological responses to a soluble recombinant chimeric Plasmodium vivax circumsporozoite protein in VK210 and VK247 population. / Cheng, Yang; Ito, Daisuke; Sattabongkot, Jetsumon; Lim, Chae Seung; Kong, Deok Hoon; Ha, Kwon Soo; Wang, Bo; Tsuboi, Takafumi; Han, Eun Taek.

In: Malaria Journal, Vol. 12, No. 1, 323, 17.09.2013.

Research output: Contribution to journalArticle

Cheng, Yang ; Ito, Daisuke ; Sattabongkot, Jetsumon ; Lim, Chae Seung ; Kong, Deok Hoon ; Ha, Kwon Soo ; Wang, Bo ; Tsuboi, Takafumi ; Han, Eun Taek. / Serological responses to a soluble recombinant chimeric Plasmodium vivax circumsporozoite protein in VK210 and VK247 population. In: Malaria Journal. 2013 ; Vol. 12, No. 1.
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abstract = "Background: Circumsporozoite protein (CSP) is essential for sporozoite formation and sporozoite invasion into human hepatocyte. Previously, a recombinant P. vivax CSP based on chimeric repeats (rPvCSP-c) representing two major alleles VK210 and VK247 within central region has been designed. Naturally acquired humoral immune responses study show that antigenicity of rPvCSP-c was much higher than that of native strain. However, the serologic reactivity of rPvCSP-c was still unclear in detail. Methods. In present study, recognition of rPvCSP-c in vivax malaria typed VK210 and VK247 alleles was assessed. VK210 typed and VK247 typed sera from adult residents reacted specifically with rPvCSP-c using protein array and immunoblot assay. Additionally, anti-rPvCSP-c serum recognized the fixed VK210 and VK247 sporozoites by immunofluorescence assay. Furthermore, statistic analysis was performed for correlational detection. Results: The rPvCSP-c reacted with both VK210 typed and VK247 typed P. vivax infected patient sera and anti-rPvCSP-c immune serum also reacted with VK210 and VK247 sporozoite parasites of P. vivax specifically. There was a positive correlation between increased antibody level, age of patients and also associated with pvcsp repeat number, although the level of responses did vary considerably in their reactivity to the rPvCSP-c from negative to very high level within each age group. Conclusions: These data confirmed the serologic reactivity of the novel rPvCSP-c in exposed both VK210 and VK247 populations. These results strongly suggested that this recombinant CSP was biologically active and potently immunogenic across major strains and raised the prospect that this protein could be used as serologic marker.",
author = "Yang Cheng and Daisuke Ito and Jetsumon Sattabongkot and Lim, {Chae Seung} and Kong, {Deok Hoon} and Ha, {Kwon Soo} and Bo Wang and Takafumi Tsuboi and Han, {Eun Taek}",
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AU - Cheng, Yang

AU - Ito, Daisuke

AU - Sattabongkot, Jetsumon

AU - Lim, Chae Seung

AU - Kong, Deok Hoon

AU - Ha, Kwon Soo

AU - Wang, Bo

AU - Tsuboi, Takafumi

AU - Han, Eun Taek

PY - 2013/9/17

Y1 - 2013/9/17

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AB - Background: Circumsporozoite protein (CSP) is essential for sporozoite formation and sporozoite invasion into human hepatocyte. Previously, a recombinant P. vivax CSP based on chimeric repeats (rPvCSP-c) representing two major alleles VK210 and VK247 within central region has been designed. Naturally acquired humoral immune responses study show that antigenicity of rPvCSP-c was much higher than that of native strain. However, the serologic reactivity of rPvCSP-c was still unclear in detail. Methods. In present study, recognition of rPvCSP-c in vivax malaria typed VK210 and VK247 alleles was assessed. VK210 typed and VK247 typed sera from adult residents reacted specifically with rPvCSP-c using protein array and immunoblot assay. Additionally, anti-rPvCSP-c serum recognized the fixed VK210 and VK247 sporozoites by immunofluorescence assay. Furthermore, statistic analysis was performed for correlational detection. Results: The rPvCSP-c reacted with both VK210 typed and VK247 typed P. vivax infected patient sera and anti-rPvCSP-c immune serum also reacted with VK210 and VK247 sporozoite parasites of P. vivax specifically. There was a positive correlation between increased antibody level, age of patients and also associated with pvcsp repeat number, although the level of responses did vary considerably in their reactivity to the rPvCSP-c from negative to very high level within each age group. Conclusions: These data confirmed the serologic reactivity of the novel rPvCSP-c in exposed both VK210 and VK247 populations. These results strongly suggested that this recombinant CSP was biologically active and potently immunogenic across major strains and raised the prospect that this protein could be used as serologic marker.

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