Seroprevalence and phylogenetic analysis of Orientia tsutsugamushi from Apodemus peninsulae in Korea

Luck Ju Baek, Won Ik Lee, Jin Won Song, Sung Ho Rhu, Soo Yong Chung, Sung Sil Moon, Kwang Sook Park, Sun-Ho Kee, Ki-Joon Song

Research output: Contribution to journalArticle

Abstract

To investigate the seroprevalence of the Orientia tsutsugamushi infection of Apodemus peninsulae and genomic variations in O. tsutsugamushi isolates, 246 A. peninsulae were trapped in 14 mountainous areas approximately 500 meter above sea level in Korea during the period of 1997 and 2000. Seropositive rate of O. tsutsugamushi among A. peninsulae was 31.8% in Kyunggi, 8.2% in Chunbuk and 7.1% in Kangwon provinces by microimmiinofluorescent test. The 56 kDa protein gene was amplified by PCR in the spleens of seropositive A. peninsulae. Two amplicons from seropositive A. peninsulae were sequenced and their phylogeny was analysed on the basis of sequence homology. The 56 kDa genes of A. peninsulae 98-12 strain and A. peninsulae 98-16 strain showed 98.7% nucleotide homology and 96.6% amino acid similarity. A. peninsulae 98-12 and A. peninsulae 98-16 strain were related to Kuroki, Boryong and Karp strains showing 93.3-92.2%, and 87.1-84.6% homologies in nucleotide and amino acids levels, respectively. In the phylogenetic analysis, A. peninsulae 98-12 and A. peninsulae 98-16 strain formed a distinct group with Boryong, Kuroki and Nishino strains and were clearly distinguished from other genetic groups. The results suggest that A. peninsulae might be an important reservoir of O. tsutsugamushi in Korea.

Original languageEnglish
Pages (from-to)307-313
Number of pages7
JournalJournal of Bacteriology and Virology
Volume32
Issue number4
Publication statusPublished - 2002 Dec 1

Fingerprint

Orientia tsutsugamushi
Murinae
Seroepidemiologic Studies
Korea
Nucleotides
Amino Acids
Phylogeny
Sequence Homology
Oceans and Seas
Spleen
Polymerase Chain Reaction
Infection
Genes
Proteins

Keywords

  • Apodemus peninsulae
  • Orientia tsutsugamushi

ASJC Scopus subject areas

  • Microbiology
  • Virology
  • Immunology

Cite this

Seroprevalence and phylogenetic analysis of Orientia tsutsugamushi from Apodemus peninsulae in Korea. / Baek, Luck Ju; Lee, Won Ik; Song, Jin Won; Rhu, Sung Ho; Chung, Soo Yong; Moon, Sung Sil; Park, Kwang Sook; Kee, Sun-Ho; Song, Ki-Joon.

In: Journal of Bacteriology and Virology, Vol. 32, No. 4, 01.12.2002, p. 307-313.

Research output: Contribution to journalArticle

Baek, Luck Ju ; Lee, Won Ik ; Song, Jin Won ; Rhu, Sung Ho ; Chung, Soo Yong ; Moon, Sung Sil ; Park, Kwang Sook ; Kee, Sun-Ho ; Song, Ki-Joon. / Seroprevalence and phylogenetic analysis of Orientia tsutsugamushi from Apodemus peninsulae in Korea. In: Journal of Bacteriology and Virology. 2002 ; Vol. 32, No. 4. pp. 307-313.
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T1 - Seroprevalence and phylogenetic analysis of Orientia tsutsugamushi from Apodemus peninsulae in Korea

AU - Baek, Luck Ju

AU - Lee, Won Ik

AU - Song, Jin Won

AU - Rhu, Sung Ho

AU - Chung, Soo Yong

AU - Moon, Sung Sil

AU - Park, Kwang Sook

AU - Kee, Sun-Ho

AU - Song, Ki-Joon

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N2 - To investigate the seroprevalence of the Orientia tsutsugamushi infection of Apodemus peninsulae and genomic variations in O. tsutsugamushi isolates, 246 A. peninsulae were trapped in 14 mountainous areas approximately 500 meter above sea level in Korea during the period of 1997 and 2000. Seropositive rate of O. tsutsugamushi among A. peninsulae was 31.8% in Kyunggi, 8.2% in Chunbuk and 7.1% in Kangwon provinces by microimmiinofluorescent test. The 56 kDa protein gene was amplified by PCR in the spleens of seropositive A. peninsulae. Two amplicons from seropositive A. peninsulae were sequenced and their phylogeny was analysed on the basis of sequence homology. The 56 kDa genes of A. peninsulae 98-12 strain and A. peninsulae 98-16 strain showed 98.7% nucleotide homology and 96.6% amino acid similarity. A. peninsulae 98-12 and A. peninsulae 98-16 strain were related to Kuroki, Boryong and Karp strains showing 93.3-92.2%, and 87.1-84.6% homologies in nucleotide and amino acids levels, respectively. In the phylogenetic analysis, A. peninsulae 98-12 and A. peninsulae 98-16 strain formed a distinct group with Boryong, Kuroki and Nishino strains and were clearly distinguished from other genetic groups. The results suggest that A. peninsulae might be an important reservoir of O. tsutsugamushi in Korea.

AB - To investigate the seroprevalence of the Orientia tsutsugamushi infection of Apodemus peninsulae and genomic variations in O. tsutsugamushi isolates, 246 A. peninsulae were trapped in 14 mountainous areas approximately 500 meter above sea level in Korea during the period of 1997 and 2000. Seropositive rate of O. tsutsugamushi among A. peninsulae was 31.8% in Kyunggi, 8.2% in Chunbuk and 7.1% in Kangwon provinces by microimmiinofluorescent test. The 56 kDa protein gene was amplified by PCR in the spleens of seropositive A. peninsulae. Two amplicons from seropositive A. peninsulae were sequenced and their phylogeny was analysed on the basis of sequence homology. The 56 kDa genes of A. peninsulae 98-12 strain and A. peninsulae 98-16 strain showed 98.7% nucleotide homology and 96.6% amino acid similarity. A. peninsulae 98-12 and A. peninsulae 98-16 strain were related to Kuroki, Boryong and Karp strains showing 93.3-92.2%, and 87.1-84.6% homologies in nucleotide and amino acids levels, respectively. In the phylogenetic analysis, A. peninsulae 98-12 and A. peninsulae 98-16 strain formed a distinct group with Boryong, Kuroki and Nishino strains and were clearly distinguished from other genetic groups. The results suggest that A. peninsulae might be an important reservoir of O. tsutsugamushi in Korea.

KW - Apodemus peninsulae

KW - Orientia tsutsugamushi

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