SERS-based particle tracking and molecular imaging in live cells: Toward the monitoring of intracellular dynamics

Jongwoo Kim, Sang Hwan Nam, Dong Kwon Lim, Yung Doug Suh

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Although diverse endogenous biomolecules involved in life processes are of major interest in cell biology, there is still a lack of suitable methods for studying biomolecules within live cells without labelling. Herein, we describe a near-infrared (NIR) surface-enhanced Raman scattering (SERS)-based particle tracking technique gathering chemical information inside live cells for monitoring their intracellular dynamics. The wide-field SERS imaging spectroscopy system facilitates high temporal resolution (200 ms) under high spatial resolution (512 × 512 pixels) for one live cell. With high spatiotemporal resolution and signal-to-background ratio, we show that the Raman signal from intracellular cargoes in live cells is sporadically observed and localized to a vesicular level.

Original languageEnglish
Pages (from-to)21724-21727
Number of pages4
JournalNanoscale
Volume11
Issue number45
DOIs
Publication statusPublished - 2019 Dec 7

ASJC Scopus subject areas

  • Materials Science(all)

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