SERS imaging of HER2-overexpressed MCF7 cells using antibody-conjugated gold nanorods

Hyejin Park, Sangyeop Lee, Lingxin Chen, Eun Kyu Lee, Soon Young Shin, Young Han Lee, Sang Wook Son, Chil Hwan Oh, Joon Myong Song, Seong Ho Kang, Jaebum Choo

Research output: Contribution to journalArticle

116 Citations (Scopus)

Abstract

Antibody-conjugated gold nanorods (GNRs) have been used for the targeting and imaging of specific cancer markers expressed on the surface membrane of cancer cells. GNRs with various aspect ratios were fabricated, and their surface-enhanced Raman scattering enhancement effects were evaluated. To attach the GNRs selectively onto the targets in cancer cells, specific antibodies were immobilized on the surface of GNRs using the layer-by-layer deposition method. First, Raman reporter molecules, mercaptopyridine, were attached to the surface of GNRs, and their surface charge was modified using poly(sodium 4-styrene-sulfonate) to make the surface charge negative. Then, anti-rabbit IgGs were immobilized onto the surface of the GNRs by electrostatic interactions. HER2 markers, expressed on the cell surface, were treated with anti-HER2 primary antibodies. Finally, the functionalized nanoprobes, conjugated with secondary antibodies, were attached to the markers on cancer cells by antibody-antibody interactions. In the present study, MCF7 cells overexpressing breast cancer marker HER2 were used as the optical imaging targets. Our experimental results demonstrate the potential feasibility of antibody-conjugated GNRs for the highly sensitive targeting and imaging of biomarkers expressed on the surface membrane of cancer cells.

Original languageEnglish
Pages (from-to)7444-7449
Number of pages6
JournalPhysical Chemistry Chemical Physics
Volume11
Issue number34
DOIs
Publication statusPublished - 2009 Aug 31

Fingerprint

Nanotubes
MCF-7 Cells
antibodies
Nanorods
Gold
nanorods
gold
Imaging techniques
Antibodies
cancer
cells
Cells
markers
Neoplasms
Surface charge
Cell Membrane
Immobilized Antibodies
Nanoprobes
Membranes
Raman Spectrum Analysis

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Physics and Astronomy(all)

Cite this

Park, H., Lee, S., Chen, L., Lee, E. K., Shin, S. Y., Lee, Y. H., ... Choo, J. (2009). SERS imaging of HER2-overexpressed MCF7 cells using antibody-conjugated gold nanorods. Physical Chemistry Chemical Physics, 11(34), 7444-7449. https://doi.org/10.1039/b904592a

SERS imaging of HER2-overexpressed MCF7 cells using antibody-conjugated gold nanorods. / Park, Hyejin; Lee, Sangyeop; Chen, Lingxin; Lee, Eun Kyu; Shin, Soon Young; Lee, Young Han; Son, Sang Wook; Oh, Chil Hwan; Song, Joon Myong; Kang, Seong Ho; Choo, Jaebum.

In: Physical Chemistry Chemical Physics, Vol. 11, No. 34, 31.08.2009, p. 7444-7449.

Research output: Contribution to journalArticle

Park, H, Lee, S, Chen, L, Lee, EK, Shin, SY, Lee, YH, Son, SW, Oh, CH, Song, JM, Kang, SH & Choo, J 2009, 'SERS imaging of HER2-overexpressed MCF7 cells using antibody-conjugated gold nanorods', Physical Chemistry Chemical Physics, vol. 11, no. 34, pp. 7444-7449. https://doi.org/10.1039/b904592a
Park, Hyejin ; Lee, Sangyeop ; Chen, Lingxin ; Lee, Eun Kyu ; Shin, Soon Young ; Lee, Young Han ; Son, Sang Wook ; Oh, Chil Hwan ; Song, Joon Myong ; Kang, Seong Ho ; Choo, Jaebum. / SERS imaging of HER2-overexpressed MCF7 cells using antibody-conjugated gold nanorods. In: Physical Chemistry Chemical Physics. 2009 ; Vol. 11, No. 34. pp. 7444-7449.
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