SERS imaging of HER2-overexpressed MCF7 cells using antibody-conjugated gold nanorods

Hyejin Park, Sangyeop Lee, Lingxin Chen, Eun Kyu Lee, Soon Young Shin, Young Han Lee, Sang Wook Son, Chil Hwan Oh, Joon Myong Song, Seong Ho Kang, Jaebum Choo

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118 Citations (Scopus)

Abstract

Antibody-conjugated gold nanorods (GNRs) have been used for the targeting and imaging of specific cancer markers expressed on the surface membrane of cancer cells. GNRs with various aspect ratios were fabricated, and their surface-enhanced Raman scattering enhancement effects were evaluated. To attach the GNRs selectively onto the targets in cancer cells, specific antibodies were immobilized on the surface of GNRs using the layer-by-layer deposition method. First, Raman reporter molecules, mercaptopyridine, were attached to the surface of GNRs, and their surface charge was modified using poly(sodium 4-styrene-sulfonate) to make the surface charge negative. Then, anti-rabbit IgGs were immobilized onto the surface of the GNRs by electrostatic interactions. HER2 markers, expressed on the cell surface, were treated with anti-HER2 primary antibodies. Finally, the functionalized nanoprobes, conjugated with secondary antibodies, were attached to the markers on cancer cells by antibody-antibody interactions. In the present study, MCF7 cells overexpressing breast cancer marker HER2 were used as the optical imaging targets. Our experimental results demonstrate the potential feasibility of antibody-conjugated GNRs for the highly sensitive targeting and imaging of biomarkers expressed on the surface membrane of cancer cells.

Original languageEnglish
Pages (from-to)7444-7449
Number of pages6
JournalPhysical Chemistry Chemical Physics
Volume11
Issue number34
DOIs
Publication statusPublished - 2009 Aug 31

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Physics and Astronomy(all)

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    Park, H., Lee, S., Chen, L., Lee, E. K., Shin, S. Y., Lee, Y. H., Son, S. W., Oh, C. H., Song, J. M., Kang, S. H., & Choo, J. (2009). SERS imaging of HER2-overexpressed MCF7 cells using antibody-conjugated gold nanorods. Physical Chemistry Chemical Physics, 11(34), 7444-7449. https://doi.org/10.1039/b904592a