Sex difference and steroidal stimulation of galanin immunoreactivity in the ferret's dorsal preoptic area/anterior hypothalamus

Joong Jean Park, Michael J. Baum, Stuart A. Tobet

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

A sexually dimorphic male nucleus (MN) is seen in Nissl-stained sections from the dorsal preoptic area/anterior hypothalamus (dPOA/AH) of male, but not female, ferrets. We used immunohistochemical methods to determine whether particular neuropeptides are found in cells of the MN. A sexually dimorphic cluster of galanin-immunoreactive (IR) cells was found in the dPOA/AH of ferrets killed either on embryonic day (E) 38 or in adulthood. Significantly more galanin-IR cells were distributed in the MN and in other subregions of the dPOA/AH of intact breeding males than estrous females. The density of galanin-IR cells in the dPOA/AH was significantly reduced in adult males by castration and restored to the level of intact breeding males by dally injections of testosterone propionate (TP) for 5 weeks. The same TP treatment failed to augment the density of galanin-IR cells in the dPOA/AH of adult, ovariectomized females. Computer-assisted image analysis and grid-crossing analysis showed that the area and the number of galanin-IR fibers in the dPOA/AH were significantly greater in adult females than in males, regardless of subjects concurrent steroidal condition. A cluster of galanin-IR cells was present in the dPOA/AH of males, but not females, killed on either E34 or E38. Administration of TP between E28 and E37 significantly increased the density of galanin-IR cells in the dPOA/AH of females killed on E38, up to the level seen in control males. The results suggest that the capacity of cells located in the dPOA/AH to express galanin after adult steroid exposure is sexually differentiated by the fetal action of testostorone, or its metabolite, estradiol, in males.

Original languageEnglish
Pages (from-to)277-288
Number of pages12
JournalJournal of Comparative Neurology
Volume389
Issue number2
DOIs
Publication statusPublished - 1997 Dec 15
Externally publishedYes

Fingerprint

Anterior Hypothalamus
Galanin
Ferrets
Preoptic Area
Sex Characteristics
Testosterone Propionate
Breeding
Computer-Assisted Image Processing
Orchiectomy
Neuropeptides
Cell Nucleus
Estradiol
Steroids

Keywords

  • Bed nucleus of stria terminalis
  • Medial amygdala
  • Neuropeptide
  • Testosterone
  • Ventromedial hypothalamus

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Sex difference and steroidal stimulation of galanin immunoreactivity in the ferret's dorsal preoptic area/anterior hypothalamus. / Park, Joong Jean; Baum, Michael J.; Tobet, Stuart A.

In: Journal of Comparative Neurology, Vol. 389, No. 2, 15.12.1997, p. 277-288.

Research output: Contribution to journalArticle

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N2 - A sexually dimorphic male nucleus (MN) is seen in Nissl-stained sections from the dorsal preoptic area/anterior hypothalamus (dPOA/AH) of male, but not female, ferrets. We used immunohistochemical methods to determine whether particular neuropeptides are found in cells of the MN. A sexually dimorphic cluster of galanin-immunoreactive (IR) cells was found in the dPOA/AH of ferrets killed either on embryonic day (E) 38 or in adulthood. Significantly more galanin-IR cells were distributed in the MN and in other subregions of the dPOA/AH of intact breeding males than estrous females. The density of galanin-IR cells in the dPOA/AH was significantly reduced in adult males by castration and restored to the level of intact breeding males by dally injections of testosterone propionate (TP) for 5 weeks. The same TP treatment failed to augment the density of galanin-IR cells in the dPOA/AH of adult, ovariectomized females. Computer-assisted image analysis and grid-crossing analysis showed that the area and the number of galanin-IR fibers in the dPOA/AH were significantly greater in adult females than in males, regardless of subjects concurrent steroidal condition. A cluster of galanin-IR cells was present in the dPOA/AH of males, but not females, killed on either E34 or E38. Administration of TP between E28 and E37 significantly increased the density of galanin-IR cells in the dPOA/AH of females killed on E38, up to the level seen in control males. The results suggest that the capacity of cells located in the dPOA/AH to express galanin after adult steroid exposure is sexually differentiated by the fetal action of testostorone, or its metabolite, estradiol, in males.

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