Sheathless shape-based separation of candida albicans using a viscoelastic non-newtonian fluid

Jeonghun Nam; Hyunseul Jee; Woong Sik Jang; Jung Yoon; Borae G. Park; Seong Jae Lee; Chae Seung Lim

doi:10.3390/mi10120817

Sheathless shape-based separation of candida albicans using a viscoelastic non-newtonian fluid

Jeonghun Nam, Hyunseul Jee, Woong Sik Jang, Jung Yoon, Borae G. Park, Seong Jae Lee, Chae Seung Lim

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Rapid and accurate identification of Candida albicans from among other candida species is critical for cost-effective treatment and antifungal drug assays. Shape is a critical biomarker indicating cell type, cell cycle, and environmental conditions; however, most microfluidic techniques have been focused only on size-based particle/cell manipulation. This study demonstrates a sheathless shape-based separation of particles/cells using a viscoelastic non-Newtonian fluid. The size of C. albicans was measured at 37 °C depending on the incubation time (0 h, 1 h, and 2 h). The effects of flow rates on the flow patterns of candida cells with different shapes were examined. Finally, 2-h-incubated candida cells with germ tube formations (≥26 μm) were separated from spherical candida cells and shorter candida cells with a separation efficiency of 80.9% and a purity of 91.2% at 50 μL/min.

Original language	English
Article number	817
Journal	Micromachines
Volume	10
Issue number	12
DOIs	https://doi.org/10.3390/mi10120817
Publication status	Published - 2019 Dec 1

Keywords

Candida
Separation
Shape
Sheathless
Viscoelastic fluid

ASJC Scopus subject areas

Control and Systems Engineering
Mechanical Engineering
Electrical and Electronic Engineering

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10.3390/mi10120817

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title = "Sheathless shape-based separation of candida albicans using a viscoelastic non-newtonian fluid",

abstract = "Rapid and accurate identification of Candida albicans from among other candida species is critical for cost-effective treatment and antifungal drug assays. Shape is a critical biomarker indicating cell type, cell cycle, and environmental conditions; however, most microfluidic techniques have been focused only on size-based particle/cell manipulation. This study demonstrates a sheathless shape-based separation of particles/cells using a viscoelastic non-Newtonian fluid. The size of C. albicans was measured at 37 °C depending on the incubation time (0 h, 1 h, and 2 h). The effects of flow rates on the flow patterns of candida cells with different shapes were examined. Finally, 2-h-incubated candida cells with germ tube formations (≥26 μm) were separated from spherical candida cells and shorter candida cells with a separation efficiency of 80.9% and a purity of 91.2% at 50 μL/min.",

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author = "Jeonghun Nam and Hyunseul Jee and Jang, {Woong Sik} and Jung Yoon and Park, {Borae G.} and Lee, {Seong Jae} and Lim, {Chae Seung}",

note = "Funding Information: Funding: This research was supported by a grant from the Establish R&D Platform Project through the Korea University Medical Center and the Korea University Guro Hospital, which is funded by the Korea University Guro Hospital (grant number: O1903851).",

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AU - Yoon, Jung

AU - Park, Borae G.

AU - Lee, Seong Jae

AU - Lim, Chae Seung

N1 - Funding Information: Funding: This research was supported by a grant from the Establish R&D Platform Project through the Korea University Medical Center and the Korea University Guro Hospital, which is funded by the Korea University Guro Hospital (grant number: O1903851).

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N2 - Rapid and accurate identification of Candida albicans from among other candida species is critical for cost-effective treatment and antifungal drug assays. Shape is a critical biomarker indicating cell type, cell cycle, and environmental conditions; however, most microfluidic techniques have been focused only on size-based particle/cell manipulation. This study demonstrates a sheathless shape-based separation of particles/cells using a viscoelastic non-Newtonian fluid. The size of C. albicans was measured at 37 °C depending on the incubation time (0 h, 1 h, and 2 h). The effects of flow rates on the flow patterns of candida cells with different shapes were examined. Finally, 2-h-incubated candida cells with germ tube formations (≥26 μm) were separated from spherical candida cells and shorter candida cells with a separation efficiency of 80.9% and a purity of 91.2% at 50 μL/min.

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Sheathless shape-based separation of candida albicans using a viscoelastic non-newtonian fluid

Abstract

Keywords

ASJC Scopus subject areas

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