Sheathless shape-based separation of candida albicans using a viscoelastic non-newtonian fluid

Jeonghun Nam, Hyunseul Jee, Woong Sik Jang, Jung Yoon, Borae G. Park, Seong Jae Lee, Chae Seung Lim

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Rapid and accurate identification of Candida albicans from among other candida species is critical for cost-effective treatment and antifungal drug assays. Shape is a critical biomarker indicating cell type, cell cycle, and environmental conditions; however, most microfluidic techniques have been focused only on size-based particle/cell manipulation. This study demonstrates a sheathless shape-based separation of particles/cells using a viscoelastic non-Newtonian fluid. The size of C. albicans was measured at 37 °C depending on the incubation time (0 h, 1 h, and 2 h). The effects of flow rates on the flow patterns of candida cells with different shapes were examined. Finally, 2-h-incubated candida cells with germ tube formations (≥26 μm) were separated from spherical candida cells and shorter candida cells with a separation efficiency of 80.9% and a purity of 91.2% at 50 μL/min.

Original languageEnglish
Article number817
JournalMicromachines
Volume10
Issue number12
DOIs
Publication statusPublished - 2019 Dec 1

Keywords

  • Candida
  • Separation
  • Shape
  • Sheathless
  • Viscoelastic fluid

ASJC Scopus subject areas

  • Control and Systems Engineering
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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