SHORT VEGETATIVE PHASE (SVP) protein negatively regulates miR172 transcription via direct binding to the pri-miR172a promoter in Arabidopsis

Hyun Jung Cho, Jae Joon Kim, Jeong Hwan Lee, Wanhui Kim, Jae Hoon Jung, Chung Mo Park, Ji Hoon Ahn

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)

Abstract

Plant microRNAs (miRNAs) are non-coding RNAs that negatively regulate expression of their target genes. Although much is known about miRNA biogenesis and repression of target genes by miRNAs, the molecular mechanisms underlying the transcriptional regulation of miRNA itself are poorly understood. Here, we report that SHORT VEGETATIVE PHASE (SVP) protein is a direct transcriptional regulator of miR172. The levels of mature miR172 and pri-miR172a were anti-correlated with SVP activity. miR172a has multiple transcription start sites, among which the transcript starting with cytosine (-671 bp, relative to the mature miR172a) was a major species. EMSA and ChIP analysis demonstrated that SVP protein binds to the CArG motifs in the miR172a promoter. These results suggest that SVP protein directly regulates miR172 transcription in Arabidopsis. Crown

Original languageEnglish
Pages (from-to)2332-2337
Number of pages6
JournalFEBS Letters
Volume586
Issue number16
DOIs
Publication statusPublished - 2012 Jul 30

Keywords

  • Flowering time
  • Pri-miR172a
  • SVP
  • Transcription
  • miR172

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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