Sideroxylin (Callistemon lanceolatus) suppressed cell proliferation and increased apoptosis in ovarian cancer cells accompanied by mitochondrial dysfunction, the generation of reactive oxygen species, and an increase of lipid peroxidation

Sunwoo Park, Whasun Lim, Wonsik Jeong, Fuller W. Bazer, Dongho Lee, Gwonhwa Song

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Sideroxylin is a C-methylated flavone isolated from Callistemon lanceolatus and exerts antimicrobial activity against Staphylococcus aureus. However, the anticancer effects of sideroxylin and its intracellular signaling mechanisms have not yet been identified. Results of our study showed that sideroxylin decreased cell proliferation and increased apoptosis, causing DNA fragmentation, depolarization of the mitochondrial membrane, the generation of reactive oxygen species, and an increase of lipid peroxidation in ovarian cancer cells (ES2 and OV90 cells). Additionally, sideroxylin activated the phosphorylation of ERK1/2, JNK, P38, and MAPK proteins and the use of LY294002, U0126, SB203580, and SP600125 to block their phosphorylation, respectively, in ES2 and OV90 cells. Collectively, the results of present study indicated that sideroxylin was a novel therapeutic agent to combat the proliferation of ovarian cancer cells through the induction of mitochondrial dysfunction and the activation of PI3 K and MAPK signal transduction.

Original languageEnglish
Pages (from-to)8597-8604
Number of pages8
JournalJournal of Cellular Physiology
Volume233
Issue number11
DOIs
Publication statusPublished - 2018 Nov 1

Fingerprint

Cell proliferation
Ovarian Neoplasms
Lipid Peroxidation
Reactive Oxygen Species
Cells
Cell Proliferation
Apoptosis
Lipids
Phosphorylation
flavone
Signal transduction
2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
Depolarization
Mitochondrial Membranes
p38 Mitogen-Activated Protein Kinases
DNA Fragmentation
Staphylococcus aureus
Signal Transduction
Chemical activation
sideroxylin

Keywords

  • apoptosis
  • Callistemon lanceolatus
  • ovarian cancer
  • sideroxylin
  • signal transduction

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

Cite this

@article{3f5f08f64ac84e908f9e11df8c56acd8,
title = "Sideroxylin (Callistemon lanceolatus) suppressed cell proliferation and increased apoptosis in ovarian cancer cells accompanied by mitochondrial dysfunction, the generation of reactive oxygen species, and an increase of lipid peroxidation",
abstract = "Sideroxylin is a C-methylated flavone isolated from Callistemon lanceolatus and exerts antimicrobial activity against Staphylococcus aureus. However, the anticancer effects of sideroxylin and its intracellular signaling mechanisms have not yet been identified. Results of our study showed that sideroxylin decreased cell proliferation and increased apoptosis, causing DNA fragmentation, depolarization of the mitochondrial membrane, the generation of reactive oxygen species, and an increase of lipid peroxidation in ovarian cancer cells (ES2 and OV90 cells). Additionally, sideroxylin activated the phosphorylation of ERK1/2, JNK, P38, and MAPK proteins and the use of LY294002, U0126, SB203580, and SP600125 to block their phosphorylation, respectively, in ES2 and OV90 cells. Collectively, the results of present study indicated that sideroxylin was a novel therapeutic agent to combat the proliferation of ovarian cancer cells through the induction of mitochondrial dysfunction and the activation of PI3 K and MAPK signal transduction.",
keywords = "apoptosis, Callistemon lanceolatus, ovarian cancer, sideroxylin, signal transduction",
author = "Sunwoo Park and Whasun Lim and Wonsik Jeong and Bazer, {Fuller W.} and Dongho Lee and Gwonhwa Song",
year = "2018",
month = "11",
day = "1",
doi = "10.1002/jcp.26540",
language = "English",
volume = "233",
pages = "8597--8604",
journal = "Journal of Cellular Physiology",
issn = "0021-9541",
publisher = "Wiley-Liss Inc.",
number = "11",

}

TY - JOUR

T1 - Sideroxylin (Callistemon lanceolatus) suppressed cell proliferation and increased apoptosis in ovarian cancer cells accompanied by mitochondrial dysfunction, the generation of reactive oxygen species, and an increase of lipid peroxidation

AU - Park, Sunwoo

AU - Lim, Whasun

AU - Jeong, Wonsik

AU - Bazer, Fuller W.

AU - Lee, Dongho

AU - Song, Gwonhwa

PY - 2018/11/1

Y1 - 2018/11/1

N2 - Sideroxylin is a C-methylated flavone isolated from Callistemon lanceolatus and exerts antimicrobial activity against Staphylococcus aureus. However, the anticancer effects of sideroxylin and its intracellular signaling mechanisms have not yet been identified. Results of our study showed that sideroxylin decreased cell proliferation and increased apoptosis, causing DNA fragmentation, depolarization of the mitochondrial membrane, the generation of reactive oxygen species, and an increase of lipid peroxidation in ovarian cancer cells (ES2 and OV90 cells). Additionally, sideroxylin activated the phosphorylation of ERK1/2, JNK, P38, and MAPK proteins and the use of LY294002, U0126, SB203580, and SP600125 to block their phosphorylation, respectively, in ES2 and OV90 cells. Collectively, the results of present study indicated that sideroxylin was a novel therapeutic agent to combat the proliferation of ovarian cancer cells through the induction of mitochondrial dysfunction and the activation of PI3 K and MAPK signal transduction.

AB - Sideroxylin is a C-methylated flavone isolated from Callistemon lanceolatus and exerts antimicrobial activity against Staphylococcus aureus. However, the anticancer effects of sideroxylin and its intracellular signaling mechanisms have not yet been identified. Results of our study showed that sideroxylin decreased cell proliferation and increased apoptosis, causing DNA fragmentation, depolarization of the mitochondrial membrane, the generation of reactive oxygen species, and an increase of lipid peroxidation in ovarian cancer cells (ES2 and OV90 cells). Additionally, sideroxylin activated the phosphorylation of ERK1/2, JNK, P38, and MAPK proteins and the use of LY294002, U0126, SB203580, and SP600125 to block their phosphorylation, respectively, in ES2 and OV90 cells. Collectively, the results of present study indicated that sideroxylin was a novel therapeutic agent to combat the proliferation of ovarian cancer cells through the induction of mitochondrial dysfunction and the activation of PI3 K and MAPK signal transduction.

KW - apoptosis

KW - Callistemon lanceolatus

KW - ovarian cancer

KW - sideroxylin

KW - signal transduction

UR - http://www.scopus.com/inward/record.url?scp=85054075702&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85054075702&partnerID=8YFLogxK

U2 - 10.1002/jcp.26540

DO - 10.1002/jcp.26540

M3 - Article

C2 - 29904922

AN - SCOPUS:85054075702

VL - 233

SP - 8597

EP - 8604

JO - Journal of Cellular Physiology

JF - Journal of Cellular Physiology

SN - 0021-9541

IS - 11

ER -