SigB, an RNA polymerase sigma factor required for osmoprotection and proper differentiation of Streptomyces coelicolor

You Hee Cho, Eun-Jin Lee, Bo Eun Ahn, Jung Hye Roe

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

A gene (sigB) encoding an alternative sigma factor σB in Streptomyces coelicolor A3(2) was isolated and characterized. It encodes a polypeptide of 281 amino acids (31 546 Da) and is highly homologous to Bacillus subtilis σB. The sigB coding region is preceded by four open reading frames (ORFs): dpsA, orfA, rsbB and rsbA in sequential order. RNA analyses revealed that rsbB, rsbA and sigB constitute an operon (sigB operon). Transcripts were produced constitutively from a promoter (sigBp2) upstream of the rsbB coding region, contributing to the basal level expression of σB protein. An inducible promoter (sigBp1) resembling the catB promoter (catBp) was located between the rsbA and sigB coding regions. Transcripts from sigBp1 dramatically increased as cells differentiated on solid media, at the stationary phase in liquid media or by osmotic stresses similar to the behaviour of catBp transcripts. Both catBp and sigBp1 promoters were recognized specifically by σB-containing RNA polymerase in vitro. Disruption of the sigB gene abolished not only the differentiation-associated expression but also the osmotic induction of the catB gene, indicating that catBp is under the control of σB. The sigB mutant exhibited a similar phenotype to the catB mutant, being sensitive to hyperosmolarity, blocked in forming aerial mycelium and with skewed antibiotic production. Therefore, we conclude that σB ensures the proper differentiation and osmoprotection of S. coelicolor cells, primarily via regulation of the expression of catalase B.

Original languageEnglish
Pages (from-to)205-214
Number of pages10
JournalMolecular Microbiology
Volume42
Issue number1
DOIs
Publication statusPublished - 2001 Dec 1
Externally publishedYes

Fingerprint

Streptomyces coelicolor
Sigma Factor
DNA-Directed RNA Polymerases
Genes
RNA Polymerase II
Mycelium
Osmotic Pressure
Operon
Bacillus subtilis
Catalase
Open Reading Frames
RNA
Anti-Bacterial Agents
Phenotype
Amino Acids
Peptides

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

SigB, an RNA polymerase sigma factor required for osmoprotection and proper differentiation of Streptomyces coelicolor. / Cho, You Hee; Lee, Eun-Jin; Ahn, Bo Eun; Roe, Jung Hye.

In: Molecular Microbiology, Vol. 42, No. 1, 01.12.2001, p. 205-214.

Research output: Contribution to journalArticle

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