Signal amplification by changing counterions in conjugated Polyelectrolyte-Based FRET DNA detection

Mijeong Kang, Okhil Kumar Nag, Rati Ranjan Nayak, Sungu Hwang, Hongsuk Suh, Han Young Woo

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Two types of cationic polyfluorene copolymers (FHQ, FPQ) with a same π-conjugated structure but different counterions (bromide (BR), tetraphenylborate (PB)) were synthesized and studied as a fluorescence resonance energy transfer (FRET) donor to fluorescein-labeled DNA (ssDNA-Fl). The counterions accompanying the polymer chain for charge compensation are expected to perturb complexation with DNA and modify the fine-structure of D/A complex on molecular scale, which may influence the competition between the desirable FRET and energy-wasting charge transfer quenching. The PL quenching constant of ssDNA-Fl by Stern Volmer plot was significantly reduced in the presence of the polymers with tetraphenylborate (4.3 × 106 M 1 for FHQ-BR vs 2.2 × 106 M 1 for FHQ-PB, 2.8 × 106 M 1 for FPQ-BR vs 1.3 × 106 M 1 for FPQ-PB). The resulting FRET-induced signal was amplified 2 to 8.6 times by exchanging bromide with tetraphenylborate as a counterion, suggesting a simple way for kinetic control of energy transfer to maximize signal amplification in conjugated polymer-based FRET biosensors.

Original languageEnglish
Pages (from-to)2708-2714
Number of pages7
JournalMacromolecules
Volume42
Issue number7
DOIs
Publication statusPublished - 2009 Apr 14
Externally publishedYes

Fingerprint

Tetraphenylborate
Polyelectrolytes
Bromides
Amplification
DNA
Quenching
Polymers
Conjugated polymers
Complexation
Fluorescein
Biosensors
Energy transfer
Charge transfer
Copolymers
Kinetics
Fluorescence Resonance Energy Transfer

ASJC Scopus subject areas

  • Organic Chemistry
  • Polymers and Plastics
  • Inorganic Chemistry
  • Materials Chemistry

Cite this

Signal amplification by changing counterions in conjugated Polyelectrolyte-Based FRET DNA detection. / Kang, Mijeong; Nag, Okhil Kumar; Nayak, Rati Ranjan; Hwang, Sungu; Suh, Hongsuk; Woo, Han Young.

In: Macromolecules, Vol. 42, No. 7, 14.04.2009, p. 2708-2714.

Research output: Contribution to journalArticle

Kang, Mijeong ; Nag, Okhil Kumar ; Nayak, Rati Ranjan ; Hwang, Sungu ; Suh, Hongsuk ; Woo, Han Young. / Signal amplification by changing counterions in conjugated Polyelectrolyte-Based FRET DNA detection. In: Macromolecules. 2009 ; Vol. 42, No. 7. pp. 2708-2714.
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