Signal amplification by changing counterions in conjugated Polyelectrolyte-Based FRET DNA detection

Mijeong Kang; Okhil Kumar Nag; Rati Ranjan Nayak; Sungu Hwang; Hongsuk Suh; Han Young Woo

doi:10.1021/ma802647u

Signal amplification by changing counterions in conjugated Polyelectrolyte-Based FRET DNA detection

Mijeong Kang, Okhil Kumar Nag, Rati Ranjan Nayak, Sungu Hwang, Hongsuk Suh, Han Young Woo

Research output: Contribution to journal › Article › peer-review

38 Citations (Scopus)

Abstract

Two types of cationic polyfluorene copolymers (FHQ, FPQ) with a same π-conjugated structure but different counterions (bromide (BR), tetraphenylborate (PB)) were synthesized and studied as a fluorescence resonance energy transfer (FRET) donor to fluorescein-labeled DNA (ssDNA-Fl). The counterions accompanying the polymer chain for charge compensation are expected to perturb complexation with DNA and modify the fine-structure of D/A complex on molecular scale, which may influence the competition between the desirable FRET and energy-wasting charge transfer quenching. The PL quenching constant of ssDNA-Fl by Stern Volmer plot was significantly reduced in the presence of the polymers with tetraphenylborate (4.3 × 106 M 1 for FHQ-BR vs 2.2 × 106 M 1 for FHQ-PB, 2.8 × 106 M 1 for FPQ-BR vs 1.3 × 106 M 1 for FPQ-PB). The resulting FRET-induced signal was amplified 2 to 8.6 times by exchanging bromide with tetraphenylborate as a counterion, suggesting a simple way for kinetic control of energy transfer to maximize signal amplification in conjugated polymer-based FRET biosensors.

Original language	English
Pages (from-to)	2708-2714
Number of pages	7
Journal	Macromolecules
Volume	42
Issue number	7
DOIs	https://doi.org/10.1021/ma802647u
Publication status	Published - 2009 Apr 14
Externally published	Yes

ASJC Scopus subject areas

Organic Chemistry
Polymers and Plastics
Inorganic Chemistry
Materials Chemistry

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title = "Signal amplification by changing counterions in conjugated Polyelectrolyte-Based FRET DNA detection",

abstract = "Two types of cationic polyfluorene copolymers (FHQ, FPQ) with a same π-conjugated structure but different counterions (bromide (BR), tetraphenylborate (PB)) were synthesized and studied as a fluorescence resonance energy transfer (FRET) donor to fluorescein-labeled DNA (ssDNA-Fl). The counterions accompanying the polymer chain for charge compensation are expected to perturb complexation with DNA and modify the fine-structure of D/A complex on molecular scale, which may influence the competition between the desirable FRET and energy-wasting charge transfer quenching. The PL quenching constant of ssDNA-Fl by Stern Volmer plot was significantly reduced in the presence of the polymers with tetraphenylborate (4.3 × 106 M 1 for FHQ-BR vs 2.2 × 106 M 1 for FHQ-PB, 2.8 × 106 M 1 for FPQ-BR vs 1.3 × 106 M 1 for FPQ-PB). The resulting FRET-induced signal was amplified 2 to 8.6 times by exchanging bromide with tetraphenylborate as a counterion, suggesting a simple way for kinetic control of energy transfer to maximize signal amplification in conjugated polymer-based FRET biosensors.",

author = "Mijeong Kang and Nag, {Okhil Kumar} and Nayak, {Rati Ranjan} and Sungu Hwang and Hongsuk Suh and Woo, {Han Young}",

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AU - Kang, Mijeong

AU - Nag, Okhil Kumar

AU - Nayak, Rati Ranjan

AU - Hwang, Sungu

AU - Suh, Hongsuk

AU - Woo, Han Young

PY - 2009/4/14

Y1 - 2009/4/14

N2 - Two types of cationic polyfluorene copolymers (FHQ, FPQ) with a same π-conjugated structure but different counterions (bromide (BR), tetraphenylborate (PB)) were synthesized and studied as a fluorescence resonance energy transfer (FRET) donor to fluorescein-labeled DNA (ssDNA-Fl). The counterions accompanying the polymer chain for charge compensation are expected to perturb complexation with DNA and modify the fine-structure of D/A complex on molecular scale, which may influence the competition between the desirable FRET and energy-wasting charge transfer quenching. The PL quenching constant of ssDNA-Fl by Stern Volmer plot was significantly reduced in the presence of the polymers with tetraphenylborate (4.3 × 106 M 1 for FHQ-BR vs 2.2 × 106 M 1 for FHQ-PB, 2.8 × 106 M 1 for FPQ-BR vs 1.3 × 106 M 1 for FPQ-PB). The resulting FRET-induced signal was amplified 2 to 8.6 times by exchanging bromide with tetraphenylborate as a counterion, suggesting a simple way for kinetic control of energy transfer to maximize signal amplification in conjugated polymer-based FRET biosensors.

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