Abstract
Two types of cationic polyfluorene copolymers (FHQ, FPQ) with a same π-conjugated structure but different counterions (bromide (BR), tetraphenylborate (PB)) were synthesized and studied as a fluorescence resonance energy transfer (FRET) donor to fluorescein-labeled DNA (ssDNA-Fl). The counterions accompanying the polymer chain for charge compensation are expected to perturb complexation with DNA and modify the fine-structure of D/A complex on molecular scale, which may influence the competition between the desirable FRET and energy-wasting charge transfer quenching. The PL quenching constant of ssDNA-Fl by Stern Volmer plot was significantly reduced in the presence of the polymers with tetraphenylborate (4.3 × 106 M 1 for FHQ-BR vs 2.2 × 106 M 1 for FHQ-PB, 2.8 × 106 M 1 for FPQ-BR vs 1.3 × 106 M 1 for FPQ-PB). The resulting FRET-induced signal was amplified 2 to 8.6 times by exchanging bromide with tetraphenylborate as a counterion, suggesting a simple way for kinetic control of energy transfer to maximize signal amplification in conjugated polymer-based FRET biosensors.
Original language | English |
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Pages (from-to) | 2708-2714 |
Number of pages | 7 |
Journal | Macromolecules |
Volume | 42 |
Issue number | 7 |
DOIs | |
Publication status | Published - 2009 Apr 14 |
Externally published | Yes |
ASJC Scopus subject areas
- Organic Chemistry
- Polymers and Plastics
- Inorganic Chemistry
- Materials Chemistry