Abstract
Two types of cationic polyfluorene copolymers (FHQ, FPQ) with a same π-conjugated structure but different counterions (bromide (BR), tetraphenylborate (PB)) were synthesized and studied as a fluorescence resonance energy transfer (FRET) donor to fluorescein-labeled DNA (ssDNA-Fl). The counterions accompanying the polymer chain for charge compensation are expected to perturb complexation with DNA and modify the fine-structure of D/A complex on molecular scale, which may influence the competition between the desirable FRET and energy-wasting charge transfer quenching. The PL quenching constant of ssDNA-Fl by Stern Volmer plot was significantly reduced in the presence of the polymers with tetraphenylborate (4.3 × 106 M 1 for FHQ-BR vs 2.2 × 106 M 1 for FHQ-PB, 2.8 × 106 M 1 for FPQ-BR vs 1.3 × 106 M 1 for FPQ-PB). The resulting FRET-induced signal was amplified 2 to 8.6 times by exchanging bromide with tetraphenylborate as a counterion, suggesting a simple way for kinetic control of energy transfer to maximize signal amplification in conjugated polymer-based FRET biosensors.
| Original language | English |
|---|---|
| Pages (from-to) | 2708-2714 |
| Number of pages | 7 |
| Journal | Macromolecules |
| Volume | 42 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 2009 Apr 14 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Organic Chemistry
- Polymers and Plastics
- Inorganic Chemistry
- Materials Chemistry
Cite this
Signal amplification by changing counterions in conjugated Polyelectrolyte-Based FRET DNA detection. / Kang, Mijeong; Nag, Okhil Kumar; Nayak, Rati Ranjan; Hwang, Sungu; Suh, Hongsuk; Woo, Han Young.
In: Macromolecules, Vol. 42, No. 7, 14.04.2009, p. 2708-2714.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Signal amplification by changing counterions in conjugated Polyelectrolyte-Based FRET DNA detection
AU - Kang, Mijeong
AU - Nag, Okhil Kumar
AU - Nayak, Rati Ranjan
AU - Hwang, Sungu
AU - Suh, Hongsuk
AU - Woo, Han Young
PY - 2009/4/14
Y1 - 2009/4/14
N2 - Two types of cationic polyfluorene copolymers (FHQ, FPQ) with a same π-conjugated structure but different counterions (bromide (BR), tetraphenylborate (PB)) were synthesized and studied as a fluorescence resonance energy transfer (FRET) donor to fluorescein-labeled DNA (ssDNA-Fl). The counterions accompanying the polymer chain for charge compensation are expected to perturb complexation with DNA and modify the fine-structure of D/A complex on molecular scale, which may influence the competition between the desirable FRET and energy-wasting charge transfer quenching. The PL quenching constant of ssDNA-Fl by Stern Volmer plot was significantly reduced in the presence of the polymers with tetraphenylborate (4.3 × 106 M 1 for FHQ-BR vs 2.2 × 106 M 1 for FHQ-PB, 2.8 × 106 M 1 for FPQ-BR vs 1.3 × 106 M 1 for FPQ-PB). The resulting FRET-induced signal was amplified 2 to 8.6 times by exchanging bromide with tetraphenylborate as a counterion, suggesting a simple way for kinetic control of energy transfer to maximize signal amplification in conjugated polymer-based FRET biosensors.
AB - Two types of cationic polyfluorene copolymers (FHQ, FPQ) with a same π-conjugated structure but different counterions (bromide (BR), tetraphenylborate (PB)) were synthesized and studied as a fluorescence resonance energy transfer (FRET) donor to fluorescein-labeled DNA (ssDNA-Fl). The counterions accompanying the polymer chain for charge compensation are expected to perturb complexation with DNA and modify the fine-structure of D/A complex on molecular scale, which may influence the competition between the desirable FRET and energy-wasting charge transfer quenching. The PL quenching constant of ssDNA-Fl by Stern Volmer plot was significantly reduced in the presence of the polymers with tetraphenylborate (4.3 × 106 M 1 for FHQ-BR vs 2.2 × 106 M 1 for FHQ-PB, 2.8 × 106 M 1 for FPQ-BR vs 1.3 × 106 M 1 for FPQ-PB). The resulting FRET-induced signal was amplified 2 to 8.6 times by exchanging bromide with tetraphenylborate as a counterion, suggesting a simple way for kinetic control of energy transfer to maximize signal amplification in conjugated polymer-based FRET biosensors.
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U2 - 10.1021/ma802647u
DO - 10.1021/ma802647u
M3 - Article
AN - SCOPUS:66649083471
VL - 42
SP - 2708
EP - 2714
JO - Macromolecules
JF - Macromolecules
SN - 0024-9297
IS - 7
ER -