Signaling pathways for TNF production induced by human aminoacyl-tRNA synthetase-associating factor, p43

Heonyong Park, Sang Gyu Park, Junghee Kim, Young-Gyu Ko, Sunghoon Kim

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

The p43 protein is associated with human macromolecular aminoacyl tRNA synthetase complex and secreted to up-regulate diverse proinflammatory genes including TNF. Here we focused on the p43-induced TNF production and determined its responsible signal pathway. The p43-induced TNF production was mediated by the activation of MAPK family members, ERK and p38 MAPK, and by IκB degradation leading to the activation of NFκB. We also studied the upstream molecules for ERK and p38 MAPK by using a variety of inhibitors. The inhibitors for protein kinase C (PKC) and phospholipase C (PLC) prevented the p43-induced TNF production. Interestingly, all of the effective drugs inhibited the ERK activity, while the drugs had no effects on p38 MAPK activity and IκB degradation. Together, the p43-induced TNF production was controlled by NFkB, p38 MAPK, and ERK that is dependent on the activities of PLC and PKC.

Original languageEnglish
Pages (from-to)148-153
Number of pages6
JournalCytokine
Volume20
Issue number4
DOIs
Publication statusPublished - 2002 Nov 1

Fingerprint

Amino Acyl-tRNA Synthetases
p38 Mitogen-Activated Protein Kinases
Type C Phospholipases
Protein Kinase C
Chemical activation
Degradation
Pharmaceutical Preparations
Signal Transduction
Up-Regulation
Genes
Molecules
Proteins

Keywords

  • Endothelial monocyte-activating polypeptide II
  • Mitogen-activated protein kinase
  • NF-κB
  • Phospholipase C
  • Protein kinase C

ASJC Scopus subject areas

  • Endocrinology
  • Molecular Biology
  • Immunology
  • Immunology and Allergy

Cite this

Signaling pathways for TNF production induced by human aminoacyl-tRNA synthetase-associating factor, p43. / Park, Heonyong; Park, Sang Gyu; Kim, Junghee; Ko, Young-Gyu; Kim, Sunghoon.

In: Cytokine, Vol. 20, No. 4, 01.11.2002, p. 148-153.

Research output: Contribution to journalArticle

Park, Heonyong ; Park, Sang Gyu ; Kim, Junghee ; Ko, Young-Gyu ; Kim, Sunghoon. / Signaling pathways for TNF production induced by human aminoacyl-tRNA synthetase-associating factor, p43. In: Cytokine. 2002 ; Vol. 20, No. 4. pp. 148-153.
@article{7e4b76a09df34e839cf0f600e753e3a2,
title = "Signaling pathways for TNF production induced by human aminoacyl-tRNA synthetase-associating factor, p43",
abstract = "The p43 protein is associated with human macromolecular aminoacyl tRNA synthetase complex and secreted to up-regulate diverse proinflammatory genes including TNF. Here we focused on the p43-induced TNF production and determined its responsible signal pathway. The p43-induced TNF production was mediated by the activation of MAPK family members, ERK and p38 MAPK, and by IκB degradation leading to the activation of NFκB. We also studied the upstream molecules for ERK and p38 MAPK by using a variety of inhibitors. The inhibitors for protein kinase C (PKC) and phospholipase C (PLC) prevented the p43-induced TNF production. Interestingly, all of the effective drugs inhibited the ERK activity, while the drugs had no effects on p38 MAPK activity and IκB degradation. Together, the p43-induced TNF production was controlled by NFkB, p38 MAPK, and ERK that is dependent on the activities of PLC and PKC.",
keywords = "Endothelial monocyte-activating polypeptide II, Mitogen-activated protein kinase, NF-κB, Phospholipase C, Protein kinase C",
author = "Heonyong Park and Park, {Sang Gyu} and Junghee Kim and Young-Gyu Ko and Sunghoon Kim",
year = "2002",
month = "11",
day = "1",
doi = "10.1006/cyto.2002.1992",
language = "English",
volume = "20",
pages = "148--153",
journal = "Cytokine",
issn = "1043-4666",
publisher = "Academic Press Inc.",
number = "4",

}

TY - JOUR

T1 - Signaling pathways for TNF production induced by human aminoacyl-tRNA synthetase-associating factor, p43

AU - Park, Heonyong

AU - Park, Sang Gyu

AU - Kim, Junghee

AU - Ko, Young-Gyu

AU - Kim, Sunghoon

PY - 2002/11/1

Y1 - 2002/11/1

N2 - The p43 protein is associated with human macromolecular aminoacyl tRNA synthetase complex and secreted to up-regulate diverse proinflammatory genes including TNF. Here we focused on the p43-induced TNF production and determined its responsible signal pathway. The p43-induced TNF production was mediated by the activation of MAPK family members, ERK and p38 MAPK, and by IκB degradation leading to the activation of NFκB. We also studied the upstream molecules for ERK and p38 MAPK by using a variety of inhibitors. The inhibitors for protein kinase C (PKC) and phospholipase C (PLC) prevented the p43-induced TNF production. Interestingly, all of the effective drugs inhibited the ERK activity, while the drugs had no effects on p38 MAPK activity and IκB degradation. Together, the p43-induced TNF production was controlled by NFkB, p38 MAPK, and ERK that is dependent on the activities of PLC and PKC.

AB - The p43 protein is associated with human macromolecular aminoacyl tRNA synthetase complex and secreted to up-regulate diverse proinflammatory genes including TNF. Here we focused on the p43-induced TNF production and determined its responsible signal pathway. The p43-induced TNF production was mediated by the activation of MAPK family members, ERK and p38 MAPK, and by IκB degradation leading to the activation of NFκB. We also studied the upstream molecules for ERK and p38 MAPK by using a variety of inhibitors. The inhibitors for protein kinase C (PKC) and phospholipase C (PLC) prevented the p43-induced TNF production. Interestingly, all of the effective drugs inhibited the ERK activity, while the drugs had no effects on p38 MAPK activity and IκB degradation. Together, the p43-induced TNF production was controlled by NFkB, p38 MAPK, and ERK that is dependent on the activities of PLC and PKC.

KW - Endothelial monocyte-activating polypeptide II

KW - Mitogen-activated protein kinase

KW - NF-κB

KW - Phospholipase C

KW - Protein kinase C

UR - http://www.scopus.com/inward/record.url?scp=0042021576&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0042021576&partnerID=8YFLogxK

U2 - 10.1006/cyto.2002.1992

DO - 10.1006/cyto.2002.1992

M3 - Article

C2 - 12543078

AN - SCOPUS:0042021576

VL - 20

SP - 148

EP - 153

JO - Cytokine

JF - Cytokine

SN - 1043-4666

IS - 4

ER -