Abstract
Background/Aims: Hepatitis C virus (HCV) RNA testing can be performed using qualitative or quantitative assays, and it is still unclear which is more useful as a primary test in patients positive for anti-HCV. The present study evaluated the usefulness of anti-HCV signal-to-cutoff ratio (S/CO ratio) for predicting HCV RNA results. Methods: Patients on whom a qualitative HCV RNA test was performed due to a positive anti-HCV enzyme immunoassay were enrolled. Patients were divided into viremia and no-viremia groups according to HCV RNA results. Receiver-operating characteristic (ROC) curve analysis was performed to evaluate the diagnostic accuracy of anti-HCV S/CO for a diagnosis of viremia. Results: In total, 487 patients were enrolled. HCV RNA was positive in 301 subjects (61.8%). Age, serum ALT level, and anti-HCV S/CO ratio were significantly different between the viremia and no-viremia groups. By ROC curve analysis, anti-HCV S/CO ratio (area, 0.989; 95% confidence interval, 0.981 to 0.998) accurately predicted the presence of viremia, with a cutoff value of 10.9 (sensitivity, 94.4%; specificity, 97.3%). Conclusions: Anti-HCV S/CO ratio was found to be highly accurate at predicting HCV viremia. The anti-HCV S/CO ratio can be used to determine whether a quantitative or qualitative HCV RNA test should be used to confirm HCV viremia in patients with a positive anti-HCV by the following criteria: if the anti-HCV S/CO ratio is <10.9, a qualitative HCV RNA test can be used, and if the anti-HCV S/CO ratio is ≥10.9 a quantitative HCV RNA test can be performed.
| Original language | English |
|---|---|
| Pages (from-to) | 302-308 |
| Number of pages | 7 |
| Journal | Korean Journal of Internal Medicine |
| Volume | 24 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2009 Dec 21 |
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Keywords
- Antibody titer
- Enzyme immunoassay
- Hepatitis C virus
- Viremia
ASJC Scopus subject areas
- Internal Medicine
Cite this
Significance of anti-HCV signal-to-cutoff ratio in predicting hepatitis C viremia. / Seo, Yeon Seok; Jung, Eun Suk; Kim, Jeong Han; Jung, Young Kul; Kim, Ji Hoon; An, Hyonggin; Yim, Hyung Joon; Yeon, Jong Eun; Byun, Kwan Soo; Kim, Chang Duck; Ryu, Ho Sang; Um, Soon-Ho.
In: Korean Journal of Internal Medicine, Vol. 24, No. 4, 21.12.2009, p. 302-308.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Significance of anti-HCV signal-to-cutoff ratio in predicting hepatitis C viremia
AU - Seo, Yeon Seok
AU - Jung, Eun Suk
AU - Kim, Jeong Han
AU - Jung, Young Kul
AU - Kim, Ji Hoon
AU - An, Hyonggin
AU - Yim, Hyung Joon
AU - Yeon, Jong Eun
AU - Byun, Kwan Soo
AU - Kim, Chang Duck
AU - Ryu, Ho Sang
AU - Um, Soon-Ho
PY - 2009/12/21
Y1 - 2009/12/21
N2 - Background/Aims: Hepatitis C virus (HCV) RNA testing can be performed using qualitative or quantitative assays, and it is still unclear which is more useful as a primary test in patients positive for anti-HCV. The present study evaluated the usefulness of anti-HCV signal-to-cutoff ratio (S/CO ratio) for predicting HCV RNA results. Methods: Patients on whom a qualitative HCV RNA test was performed due to a positive anti-HCV enzyme immunoassay were enrolled. Patients were divided into viremia and no-viremia groups according to HCV RNA results. Receiver-operating characteristic (ROC) curve analysis was performed to evaluate the diagnostic accuracy of anti-HCV S/CO for a diagnosis of viremia. Results: In total, 487 patients were enrolled. HCV RNA was positive in 301 subjects (61.8%). Age, serum ALT level, and anti-HCV S/CO ratio were significantly different between the viremia and no-viremia groups. By ROC curve analysis, anti-HCV S/CO ratio (area, 0.989; 95% confidence interval, 0.981 to 0.998) accurately predicted the presence of viremia, with a cutoff value of 10.9 (sensitivity, 94.4%; specificity, 97.3%). Conclusions: Anti-HCV S/CO ratio was found to be highly accurate at predicting HCV viremia. The anti-HCV S/CO ratio can be used to determine whether a quantitative or qualitative HCV RNA test should be used to confirm HCV viremia in patients with a positive anti-HCV by the following criteria: if the anti-HCV S/CO ratio is <10.9, a qualitative HCV RNA test can be used, and if the anti-HCV S/CO ratio is ≥10.9 a quantitative HCV RNA test can be performed.
AB - Background/Aims: Hepatitis C virus (HCV) RNA testing can be performed using qualitative or quantitative assays, and it is still unclear which is more useful as a primary test in patients positive for anti-HCV. The present study evaluated the usefulness of anti-HCV signal-to-cutoff ratio (S/CO ratio) for predicting HCV RNA results. Methods: Patients on whom a qualitative HCV RNA test was performed due to a positive anti-HCV enzyme immunoassay were enrolled. Patients were divided into viremia and no-viremia groups according to HCV RNA results. Receiver-operating characteristic (ROC) curve analysis was performed to evaluate the diagnostic accuracy of anti-HCV S/CO for a diagnosis of viremia. Results: In total, 487 patients were enrolled. HCV RNA was positive in 301 subjects (61.8%). Age, serum ALT level, and anti-HCV S/CO ratio were significantly different between the viremia and no-viremia groups. By ROC curve analysis, anti-HCV S/CO ratio (area, 0.989; 95% confidence interval, 0.981 to 0.998) accurately predicted the presence of viremia, with a cutoff value of 10.9 (sensitivity, 94.4%; specificity, 97.3%). Conclusions: Anti-HCV S/CO ratio was found to be highly accurate at predicting HCV viremia. The anti-HCV S/CO ratio can be used to determine whether a quantitative or qualitative HCV RNA test should be used to confirm HCV viremia in patients with a positive anti-HCV by the following criteria: if the anti-HCV S/CO ratio is <10.9, a qualitative HCV RNA test can be used, and if the anti-HCV S/CO ratio is ≥10.9 a quantitative HCV RNA test can be performed.
KW - Antibody titer
KW - Enzyme immunoassay
KW - Hepatitis C virus
KW - Viremia
UR - http://www.scopus.com/inward/record.url?scp=72049128038&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=72049128038&partnerID=8YFLogxK
U2 - 10.3904/kjim.2009.24.4.302
DO - 10.3904/kjim.2009.24.4.302
M3 - Article
C2 - 19949727
AN - SCOPUS:72049128038
VL - 24
SP - 302
EP - 308
JO - Korean Journal of Internal Medicine
JF - Korean Journal of Internal Medicine
SN - 0494-4712
IS - 4
ER -