Significant enhancement of direct electric communication across enzyme-electrode interface via nano-patterning of synthetic glucose dehydrogenase on spatially tunable gold nanoparticle (AuNP)-modified electrode

Hyeryeong Lee, Yoo Seok Lee, Soo Kyung Lee, Seungwoo Baek, In-Geol Choi, Jae Hyung Jang, In Seop Chang

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In this study, the effect of inter-enzyme steric hindrance that occurs during enzyme immobilization on the electrode, on direct electrical communications of enzyme with electrode was investigated via nano-patterning of enzymes on the electrode. Here, the nano-patterning of enzymes was achieved through the combination of DET-capable enzyme that was produced via fusion of site-specific gold binding peptide (GBP) to catalytic subunit of enzyme and gold nanoparticle (AuNP) array with highly tunable dimensions of AuNPs, resulting in spatially controllable enzyme-electrode. The nano-scale spatial control between immobilized enzymes on the highly tuned AuNPs shows different DET efficiency across the enzyme-electrode interface, showing 18.47% of maximum electron recovery which is 3.2-fold enhanced electron recovery efficiency compared to spatially non-controlled enzymes on the electrode where showed 5.7% of electron recovery. The result affirms that inter-enzyme interaction is a significant parameter that decides the enzyme-electrode performance.

Original languageEnglish
Pages (from-to)170-177
Number of pages8
JournalBiosensors and Bioelectronics
Volume126
DOIs
Publication statusPublished - 2019 Feb 1

Keywords

  • Charge transfer resistance
  • Direct electron transfer
  • Enzyme nano-patterning
  • Immobilization
  • Protein agglomeration

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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