Silencing by small RNAs is linked to endosomal trafficking

Youngsik Lee, Sigal Pressman, Arlise P. Andress, Kevin Kim, Jamie L. White, Justin J. Cassidy, Xin Li, Kim Lubell, Do Hwan Lim, Ik Sang Cho, Kenji Nakahara, Jonathan B. Preall, Priya Bellare, Erik J. Sontheimer, Richard W. Carthew

Research output: Contribution to journalArticle

235 Citations (Scopus)

Abstract

Small RNAs direct RNA-induced silencing complexes (RISCs) to regulate stability and translation of mRNAs. RISCs associated with target mRNAs often accumulate in discrete cytoplasmic foci known as GW-bodies. However, RISC proteins can associate with membrane compartments such as the Golgi and endoplasmic reticulum. Here, we show that GW-bodies are associated with late endosomes (multivesicular bodies, MVBs). Blocking the maturation of MVBs into lysosomes by loss of the tethering factor HPS4 (ref. 5) enhances short interfering RNA (siRNA)- and micro RNA (miRNA)-mediated silencing in Drosophila melanogaster and humans. It also triggers over-accumulation of GW-bodies. Blocking MVB formation by ESCRT (endosomal sorting complex required for transport) depletion results in impaired miRNA silencing and loss of GW-bodies. These results indicate that active RISCs are physically and functionally coupled to MVBs. We further show that MVBs promote the competence of RISCs in loading small RNAs. We suggest that the recycling of RISCs is promoted by MVBs, resulting in RISCs more effectively engaging with small RNA effectors and possibly target RNAs. It may provide a means to enhance the dynamics of RNA silencing in the cytoplasm.

Original languageEnglish
Pages (from-to)1150-1156
Number of pages7
JournalNature Cell Biology
Volume11
Issue number9
DOIs
Publication statusPublished - 2009 Aug 17

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RNA-Induced Silencing Complex
Multivesicular Bodies
RNA Interference
RNA
MicroRNAs
Endosomal Sorting Complexes Required for Transport
Messenger RNA
Endosomes
RNA Stability
Recycling
Protein Biosynthesis
Lysosomes
Drosophila melanogaster
Endoplasmic Reticulum
Mental Competency
Small Interfering RNA
Cytoplasm
Membranes

ASJC Scopus subject areas

  • Cell Biology

Cite this

Lee, Y., Pressman, S., Andress, A. P., Kim, K., White, J. L., Cassidy, J. J., ... Carthew, R. W. (2009). Silencing by small RNAs is linked to endosomal trafficking. Nature Cell Biology, 11(9), 1150-1156. https://doi.org/10.1038/ncb1930

Silencing by small RNAs is linked to endosomal trafficking. / Lee, Youngsik; Pressman, Sigal; Andress, Arlise P.; Kim, Kevin; White, Jamie L.; Cassidy, Justin J.; Li, Xin; Lubell, Kim; Lim, Do Hwan; Cho, Ik Sang; Nakahara, Kenji; Preall, Jonathan B.; Bellare, Priya; Sontheimer, Erik J.; Carthew, Richard W.

In: Nature Cell Biology, Vol. 11, No. 9, 17.08.2009, p. 1150-1156.

Research output: Contribution to journalArticle

Lee, Y, Pressman, S, Andress, AP, Kim, K, White, JL, Cassidy, JJ, Li, X, Lubell, K, Lim, DH, Cho, IS, Nakahara, K, Preall, JB, Bellare, P, Sontheimer, EJ & Carthew, RW 2009, 'Silencing by small RNAs is linked to endosomal trafficking', Nature Cell Biology, vol. 11, no. 9, pp. 1150-1156. https://doi.org/10.1038/ncb1930
Lee Y, Pressman S, Andress AP, Kim K, White JL, Cassidy JJ et al. Silencing by small RNAs is linked to endosomal trafficking. Nature Cell Biology. 2009 Aug 17;11(9):1150-1156. https://doi.org/10.1038/ncb1930
Lee, Youngsik ; Pressman, Sigal ; Andress, Arlise P. ; Kim, Kevin ; White, Jamie L. ; Cassidy, Justin J. ; Li, Xin ; Lubell, Kim ; Lim, Do Hwan ; Cho, Ik Sang ; Nakahara, Kenji ; Preall, Jonathan B. ; Bellare, Priya ; Sontheimer, Erik J. ; Carthew, Richard W. / Silencing by small RNAs is linked to endosomal trafficking. In: Nature Cell Biology. 2009 ; Vol. 11, No. 9. pp. 1150-1156.
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