Silibinin inhibits TPA-induced cell migration and MMP-9 expression in thyroid and breast cancer cells

Soo Jin Oh, Seung Pil Jung, Jeonghun Han, Sangmin Kim, Jee Soo Kim, Seok Jin Nam, Jeong Eon Lee, Jung Han Kim

    Research output: Contribution to journalArticlepeer-review

    30 Citations (Scopus)

    Abstract

    Matrix metalloproteinases (MMPs) play an important role in cancer metastasis, cell migration and invasion. Herein, we investigated the effects of silibinin on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced cell migration and MMP-9 expression in thyroid and breast cancer cells. Our results revealed that the levels of MMP-9 mRNA and protein expression were significantly increased by TPA but not MMP-2 in TPC-1 and MCF7 cells. To verify the regulatory mechanism of TPA-induced MMP-9 expression, we treated TPC-1 and MCF7 cells with the MEK1/2 inhibitor, UO126, and TPA-induced MMP-9 expression was significantly decreased. We also found that TPA-induced cell migration and MMP-9 expression was significantly decreased by silibinin. In addition, TPA-induced phosphorylation of MEK and ERK was also inhibited by silibinin. Taken together, we suggest that silibinin suppresses TPA-induced cell migration and MMP-9 expression through the MEK/ERK-dependent pathway in thyroid and breast cancer cells.

    Original languageEnglish
    Pages (from-to)1343-1348
    Number of pages6
    JournalOncology reports
    Volume29
    Issue number4
    DOIs
    Publication statusPublished - 2013 Apr

    Keywords

    • Breast cancer
    • MMP-9
    • Phorbol ester
    • Silibinin
    • Thyroid cancer

    ASJC Scopus subject areas

    • Oncology
    • Cancer Research

    Fingerprint

    Dive into the research topics of 'Silibinin inhibits TPA-induced cell migration and MMP-9 expression in thyroid and breast cancer cells'. Together they form a unique fingerprint.

    Cite this