Silibinin inhibits TPA-induced cell migration and MMP-9 expression in thyroid and breast cancer cells

Soo Jin Oh, Seung Pil Jung, Jeonghun Han, Sangmin Kim, Jee Soo Kim, Seok Jin Nam, Jeong Eon Lee, Jung Han Kim

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Matrix metalloproteinases (MMPs) play an important role in cancer metastasis, cell migration and invasion. Herein, we investigated the effects of silibinin on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced cell migration and MMP-9 expression in thyroid and breast cancer cells. Our results revealed that the levels of MMP-9 mRNA and protein expression were significantly increased by TPA but not MMP-2 in TPC-1 and MCF7 cells. To verify the regulatory mechanism of TPA-induced MMP-9 expression, we treated TPC-1 and MCF7 cells with the MEK1/2 inhibitor, UO126, and TPA-induced MMP-9 expression was significantly decreased. We also found that TPA-induced cell migration and MMP-9 expression was significantly decreased by silibinin. In addition, TPA-induced phosphorylation of MEK and ERK was also inhibited by silibinin. Taken together, we suggest that silibinin suppresses TPA-induced cell migration and MMP-9 expression through the MEK/ERK-dependent pathway in thyroid and breast cancer cells.

Original languageEnglish
Pages (from-to)1343-1348
Number of pages6
JournalOncology reports
Volume29
Issue number4
DOIs
Publication statusPublished - 2013 Apr
Externally publishedYes

Keywords

  • Breast cancer
  • MMP-9
  • Phorbol ester
  • Silibinin
  • Thyroid cancer

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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  • Cite this

    Oh, S. J., Jung, S. P., Han, J., Kim, S., Kim, J. S., Nam, S. J., Lee, J. E., & Kim, J. H. (2013). Silibinin inhibits TPA-induced cell migration and MMP-9 expression in thyroid and breast cancer cells. Oncology reports, 29(4), 1343-1348. https://doi.org/10.3892/or.2013.2252