Abstract
Silver materials have been widely used in diverse fields. However, their toxicity and their mechanism, especially in different forms, have not been studied sufficiently. Thus, cytotoxicity, apoptosis, and interleukin-1beta (IL-1b) production were investigated using macrophage-like THP-1 cells in the presence of Ag microparticles (AgMPs, 2.7 mm), Ag submicroparticles (AgSMPs, 150 nm), and Ag wires (AgWs, 274 nm65.3 mm). The levels of cytotoxicity, apoptosis, and IL-1b production by AgWs were higher than those by the other two AgSMPs and AgMPs. This trend was also observed with each step of the signaling mechanism for IL-1b production, which is a single pathway affiliated with ROS generation or lysosomal rupture or both, cathepsin B, caspase-1 (NALP3 inflammasome), and finally IL-1b production in THP-1 cells. All these results suggest that, for development of safe and effective silver materials, the shape or form of silver materials should be considered, especially for macrophage cell lines because epithelial cell lines are not overly sensitive to silver materials.
| Original language | English |
|---|---|
| Article number | e112256 |
| Journal | PLoS One |
| Volume | 9 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - 2014 Nov 14 |
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ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Medicine(all)
Cite this
Silver wire amplifies the signaling mechanism for il-1beta production more than silver submicroparticles in human monocytic THP-1 cells. / Jung, Hye Jin; Pak, Pyo June; Park, Sung Hyo; Ju, Jae Eun; Kim, Joong Su; Lee, Hoi Seon; Chung, Namhyun.
In: PLoS One, Vol. 9, No. 11, e112256, 14.11.2014.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Silver wire amplifies the signaling mechanism for il-1beta production more than silver submicroparticles in human monocytic THP-1 cells
AU - Jung, Hye Jin
AU - Pak, Pyo June
AU - Park, Sung Hyo
AU - Ju, Jae Eun
AU - Kim, Joong Su
AU - Lee, Hoi Seon
AU - Chung, Namhyun
PY - 2014/11/14
Y1 - 2014/11/14
N2 - Silver materials have been widely used in diverse fields. However, their toxicity and their mechanism, especially in different forms, have not been studied sufficiently. Thus, cytotoxicity, apoptosis, and interleukin-1beta (IL-1b) production were investigated using macrophage-like THP-1 cells in the presence of Ag microparticles (AgMPs, 2.7 mm), Ag submicroparticles (AgSMPs, 150 nm), and Ag wires (AgWs, 274 nm65.3 mm). The levels of cytotoxicity, apoptosis, and IL-1b production by AgWs were higher than those by the other two AgSMPs and AgMPs. This trend was also observed with each step of the signaling mechanism for IL-1b production, which is a single pathway affiliated with ROS generation or lysosomal rupture or both, cathepsin B, caspase-1 (NALP3 inflammasome), and finally IL-1b production in THP-1 cells. All these results suggest that, for development of safe and effective silver materials, the shape or form of silver materials should be considered, especially for macrophage cell lines because epithelial cell lines are not overly sensitive to silver materials.
AB - Silver materials have been widely used in diverse fields. However, their toxicity and their mechanism, especially in different forms, have not been studied sufficiently. Thus, cytotoxicity, apoptosis, and interleukin-1beta (IL-1b) production were investigated using macrophage-like THP-1 cells in the presence of Ag microparticles (AgMPs, 2.7 mm), Ag submicroparticles (AgSMPs, 150 nm), and Ag wires (AgWs, 274 nm65.3 mm). The levels of cytotoxicity, apoptosis, and IL-1b production by AgWs were higher than those by the other two AgSMPs and AgMPs. This trend was also observed with each step of the signaling mechanism for IL-1b production, which is a single pathway affiliated with ROS generation or lysosomal rupture or both, cathepsin B, caspase-1 (NALP3 inflammasome), and finally IL-1b production in THP-1 cells. All these results suggest that, for development of safe and effective silver materials, the shape or form of silver materials should be considered, especially for macrophage cell lines because epithelial cell lines are not overly sensitive to silver materials.
UR - http://www.scopus.com/inward/record.url?scp=84911893928&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84911893928&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0112256
DO - 10.1371/journal.pone.0112256
M3 - Article
C2 - 25396430
AN - SCOPUS:84911893928
VL - 9
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 11
M1 - e112256
ER -