TY - JOUR
T1 - Simple and accurate quantitative analysis of 20 anti-tuberculosis drugs in human plasma using liquid chromatography-electrospray ionization-tandem mass spectrometry
AU - Kim, Hyo Ji
AU - Seo, Kyung Ah
AU - Kim, Hyun Mi
AU - Jeong, Eun Sook
AU - Ghim, Jong Lyul
AU - Lee, Seung Heon
AU - Lee, Young Min
AU - Kim, Dong Hyun
AU - Shin, Jae Gook
N1 - Funding Information:
This work was supported in part by a grant funded by the Korea Centers for Disease Control and Prevention ( 2013E3100300 ), and in part by the National Research Foundation (NRF) grant funded by the Korea government ( R13-2007-023-00000-0 ).
Publisher Copyright:
© 2014 Elsevier B.V.
PY - 2015/1/5
Y1 - 2015/1/5
N2 - A simple and accurate liquid chromatography (LC)-tandem mass spectrometry (MS/MS) method for the quantitation of 20 anti-tuberculosis (anti-TB) drugs in human plasma, was developed as a tool for therapeutic drug monitoring. Two protein precipitation methods were adopted; one using methanol containing 0.13. N HCl, for precipitation of amikacin, kanamycin, streptomycin and pyrazinamide, and the other using acetonitrile, for precipitation of preamoxicillin, ciprofloxacin, clarithromycin, clofazimine, cycloserine, ethambutol, ethionamide, isoniazid, levofloxacin, linezolid, moxifloxacin, p-aminosalicylic acid (PAS), prothionamide, rifabutin, rifampin and roxithromycin. Separation was performed either on an HILIC silica column or a reversed-phase dC18 column, with a gradient elution. Detection was carried out in multiple reaction-monitoring (MRM) mode. The calibration curves were linear over a 50-fold concentration range, with correlation coefficients (r) greater than 0.9969 for all anti-TB drugs. The intra- and inter-day precision was less than 14.3%, and the accuracy ranged between 84.8 and 113.0%. The developed method was successfully applied to the identification and quantitation of anti-TB drugs in patients with multi-drug resistant TB.
AB - A simple and accurate liquid chromatography (LC)-tandem mass spectrometry (MS/MS) method for the quantitation of 20 anti-tuberculosis (anti-TB) drugs in human plasma, was developed as a tool for therapeutic drug monitoring. Two protein precipitation methods were adopted; one using methanol containing 0.13. N HCl, for precipitation of amikacin, kanamycin, streptomycin and pyrazinamide, and the other using acetonitrile, for precipitation of preamoxicillin, ciprofloxacin, clarithromycin, clofazimine, cycloserine, ethambutol, ethionamide, isoniazid, levofloxacin, linezolid, moxifloxacin, p-aminosalicylic acid (PAS), prothionamide, rifabutin, rifampin and roxithromycin. Separation was performed either on an HILIC silica column or a reversed-phase dC18 column, with a gradient elution. Detection was carried out in multiple reaction-monitoring (MRM) mode. The calibration curves were linear over a 50-fold concentration range, with correlation coefficients (r) greater than 0.9969 for all anti-TB drugs. The intra- and inter-day precision was less than 14.3%, and the accuracy ranged between 84.8 and 113.0%. The developed method was successfully applied to the identification and quantitation of anti-TB drugs in patients with multi-drug resistant TB.
KW - Anti-tuberculosis drugs
KW - Human plasma
KW - Liquid chromatography (LC)-tandem mass spectrometry (MS/MS)
KW - Multi-drug resistant tuberculosis
KW - Multiple reaction monitoring
UR - http://www.scopus.com/inward/record.url?scp=84910616115&partnerID=8YFLogxK
U2 - 10.1016/j.jpba.2014.08.026
DO - 10.1016/j.jpba.2014.08.026
M3 - Article
C2 - 25218029
AN - SCOPUS:84910616115
VL - 102
SP - 9
EP - 16
JO - Journal of Pharmaceutical and Biomedical Analysis
JF - Journal of Pharmaceutical and Biomedical Analysis
SN - 0731-7085
ER -