Simple and accurate quantitative analysis of 20 anti-tuberculosis drugs in human plasma using liquid chromatography-electrospray ionization-tandem mass spectrometry

Hyo Ji Kim, Kyung Ah Seo, Hyun Mi Kim, Eun Sook Jeong, Jong Lyul Ghim, Seung Heon Lee, Young Min Lee, Dong Hyun Kim, Jae Gook Shin

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53 Citations (Scopus)


A simple and accurate liquid chromatography (LC)-tandem mass spectrometry (MS/MS) method for the quantitation of 20 anti-tuberculosis (anti-TB) drugs in human plasma, was developed as a tool for therapeutic drug monitoring. Two protein precipitation methods were adopted; one using methanol containing 0.13. N HCl, for precipitation of amikacin, kanamycin, streptomycin and pyrazinamide, and the other using acetonitrile, for precipitation of preamoxicillin, ciprofloxacin, clarithromycin, clofazimine, cycloserine, ethambutol, ethionamide, isoniazid, levofloxacin, linezolid, moxifloxacin, p-aminosalicylic acid (PAS), prothionamide, rifabutin, rifampin and roxithromycin. Separation was performed either on an HILIC silica column or a reversed-phase dC18 column, with a gradient elution. Detection was carried out in multiple reaction-monitoring (MRM) mode. The calibration curves were linear over a 50-fold concentration range, with correlation coefficients (r) greater than 0.9969 for all anti-TB drugs. The intra- and inter-day precision was less than 14.3%, and the accuracy ranged between 84.8 and 113.0%. The developed method was successfully applied to the identification and quantitation of anti-TB drugs in patients with multi-drug resistant TB.

Original languageEnglish
Pages (from-to)9-16
Number of pages8
JournalJournal of Pharmaceutical and Biomedical Analysis
Publication statusPublished - 2015 Jan 5



  • Anti-tuberculosis drugs
  • Human plasma
  • Liquid chromatography (LC)-tandem mass spectrometry (MS/MS)
  • Multi-drug resistant tuberculosis
  • Multiple reaction monitoring

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

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