Simple and biocompatible ion beam micropatterning of a cell-repellent polymer on cell-adhesive surfaces to manipulate cell adhesion

In Tae Hwang, Chan Hee Jung, Chang Hee Jung, Jae Hak Choi, Kwanwoo Shin, Young Do Yoo

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

In this paper, the simple and biocompatible micropatterning of cell-repellent poly(N-isopropylacrylamide) (PNIPAAm) on a cell-adhesive substrate by ion beam micropatterning to control cell adhesion is described. Cell-repellent PNIPAAm films spin-coated on cell-adhesive tissue culture polystyrene (TCPS) substrates were selectively irradiated by energetic proton ions at various fluences through a pattern mask, and subsequently developed to create the micropatterns of PNIPAAm. Well-defined negative-type PNIPAAm micropatterns were successfully created on the TCPS substrates at fluences higher than 5×1014 ions/cm2, and their chemical properties were dependent on the fluence. Moreover, based on the results of the protein adsorption and in-vitro cell culture tests, 200 μm well-defined micropatterns of mammalian cells were clearly formed on the PNIPAAm-micropatterned TCPS substrates though the preferential adsorption and growth of cells on the TCPS regions due to the strong cell-repellency of PNIPAAm.

Original languageEnglish
Pages (from-to)387-393
Number of pages7
JournalJournal of Biomedical Nanotechnology
Volume12
Issue number2
DOIs
Publication statusPublished - 2016 Feb 1

Keywords

  • Cell Micropatterns
  • Ion Irradiation
  • PNIPAAm
  • Protein Adsorption
  • Tissue Culture Polystyrene

ASJC Scopus subject areas

  • Materials Science(all)
  • Bioengineering
  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Pharmaceutical Science

Fingerprint Dive into the research topics of 'Simple and biocompatible ion beam micropatterning of a cell-repellent polymer on cell-adhesive surfaces to manipulate cell adhesion'. Together they form a unique fingerprint.

Cite this