Simple and biocompatible ion beam micropatterning of a cell-repellent polymer on cell-adhesive surfaces to manipulate cell adhesion

In Tae Hwang, Chan Hee Jung, Chang Hee Jung, Jae Hak Choi, Kwanwoo Shin, Young Do Yoo

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In this paper, the simple and biocompatible micropatterning of cell-repellent poly(N-isopropylacrylamide) (PNIPAAm) on a cell-adhesive substrate by ion beam micropatterning to control cell adhesion is described. Cell-repellent PNIPAAm films spin-coated on cell-adhesive tissue culture polystyrene (TCPS) substrates were selectively irradiated by energetic proton ions at various fluences through a pattern mask, and subsequently developed to create the micropatterns of PNIPAAm. Well-defined negative-type PNIPAAm micropatterns were successfully created on the TCPS substrates at fluences higher than 5×1014 ions/cm2, and their chemical properties were dependent on the fluence. Moreover, based on the results of the protein adsorption and in-vitro cell culture tests, 200 μm well-defined micropatterns of mammalian cells were clearly formed on the PNIPAAm-micropatterned TCPS substrates though the preferential adsorption and growth of cells on the TCPS regions due to the strong cell-repellency of PNIPAAm.

Original languageEnglish
Pages (from-to)387-393
Number of pages7
JournalJournal of Biomedical Nanotechnology
Volume12
Issue number2
DOIs
Publication statusPublished - 2016 Feb 1

Fingerprint

Tissue culture
Cell adhesion
Cell Adhesion
Adhesives
Ion beams
Polystyrenes
Polymers
Ions
Substrates
Cells
Adsorption
Cell culture
Chemical properties
Masks
Protons
Tissue Adhesives
Proteins
poly-N-isopropylacrylamide
Cell Culture Techniques
Growth

Keywords

  • Cell Micropatterns
  • Ion Irradiation
  • PNIPAAm
  • Protein Adsorption
  • Tissue Culture Polystyrene

ASJC Scopus subject areas

  • Materials Science(all)
  • Bioengineering
  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Pharmaceutical Science

Cite this

Simple and biocompatible ion beam micropatterning of a cell-repellent polymer on cell-adhesive surfaces to manipulate cell adhesion. / Hwang, In Tae; Jung, Chan Hee; Jung, Chang Hee; Choi, Jae Hak; Shin, Kwanwoo; Yoo, Young Do.

In: Journal of Biomedical Nanotechnology, Vol. 12, No. 2, 01.02.2016, p. 387-393.

Research output: Contribution to journalArticle

Hwang, In Tae ; Jung, Chan Hee ; Jung, Chang Hee ; Choi, Jae Hak ; Shin, Kwanwoo ; Yoo, Young Do. / Simple and biocompatible ion beam micropatterning of a cell-repellent polymer on cell-adhesive surfaces to manipulate cell adhesion. In: Journal of Biomedical Nanotechnology. 2016 ; Vol. 12, No. 2. pp. 387-393.
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