Simple and sensitive method of microcantilever-based DNA detection using nanoparticles conjugates

Byung Hak Cha, Sang Myung Lee, Kyo Seon Hwang, Sang Kyung Kim, Yoon Sik Lee, Byeong Kwon Ju, Tae Song Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

As the real sample diagnosis using resonance-based microcantilevers, a simple method is strongly suggested that capture probe immobilized on the cantilever directly hybridize with nanoparticles conjugated detection probe. Two different dimensions of microfabricated piezoelectric cantilevers and two different sizes of nanoparticles were used in this experiment. As a result, the most suitable conditions, the 30 × 90 μm2 (width × length) of the cantilever and the 140nm of the nanoparticles, were chosen for this method. And quantitative analysis was carried out in different concentrations of nanoparticles conjugated detection probe which are 1 μg/mL, 10 μg/mL and 100 μg/mL. In addition, the sensitivity of this method was demonstrated through the SNP (single nucleotide polymorphism) detection by comparing with quantitative data of hybridization in full matched sequence at same concentration.

Original languageEnglish
Title of host publication3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008
Pages160-163
Number of pages4
DOIs
Publication statusPublished - 2008
Event3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008 - Sanya, China
Duration: 2008 Jan 62008 Jan 9

Publication series

Name3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS

Other

Other3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008
Country/TerritoryChina
CitySanya
Period08/1/608/1/9

Keywords

  • Mass enhancement and SNP
  • Microcantilever

ASJC Scopus subject areas

  • Control and Systems Engineering
  • Electrical and Electronic Engineering

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