As the real sample diagnosis using resonance-based microcantilevers, a simple method is strongly suggested that capture probe immobilized on the cantilever directly hybridize with nanoparticles conjugated detection probe. Two different dimensions of microfabricated piezoelectric cantilevers and two different sizes of nanoparticles were used in this experiment. As a result, the most suitable conditions, the 30 × 90 μm2 (width × length) of the cantilever and the 140nm of the nanoparticles, were chosen for this method. And quantitative analysis was carried out in different concentrations of nanoparticles conjugated detection probe which are 1 μg/mL, 10 μg/mL and 100 μg/mL. In addition, the sensitivity of this method was demonstrated through the SNP (single nucleotide polymorphism) detection by comparing with quantitative data of hybridization in full matched sequence at same concentration.