Simple and sensitive method of microcantilever-based DNA detection using nanoparticles conjugates

Byung Hak Cha, Sang Myung Lee, Kyo Seon Hwang, Sang Kyung Kim, Yoon S. Lee, Byeong Kwon Ju, Tae Song Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

As the real sample diagnosis using resonance-based microcantilevers, a simple method is strongly suggested that capture probe immobilized on the cantilever directly hybridize with nanoparticles conjugated detection probe. Two different dimensions of microfabricated piezoelectric cantilevers and two different sizes of nanoparticles were used in this experiment. As a result, the most suitable conditions, the 30 × 90 μm2 (width × length) of the cantilever and the 140nm of the nanoparticles, were chosen for this method. And quantitative analysis was carried out in different concentrations of nanoparticles conjugated detection probe which are 1 μg/mL, 10 μg/mL and 100 μg/mL. In addition, the sensitivity of this method was demonstrated through the SNP (single nucleotide polymorphism) detection by comparing with quantitative data of hybridization in full matched sequence at same concentration.

Original languageEnglish
Title of host publication3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS
Pages160-163
Number of pages4
DOIs
Publication statusPublished - 2008 Sep 1
Event3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008 - Sanya, China
Duration: 2008 Jan 62008 Jan 9

Other

Other3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008
CountryChina
CitySanya
Period08/1/608/1/9

Fingerprint

DNA
Nanoparticles
Nucleotides
Polymorphism
Chemical analysis
Experiments

Keywords

  • Mass enhancement and SNP
  • Microcantilever

ASJC Scopus subject areas

  • Control and Systems Engineering
  • Electrical and Electronic Engineering

Cite this

Cha, B. H., Lee, S. M., Hwang, K. S., Kim, S. K., Lee, Y. S., Ju, B. K., & Kim, T. S. (2008). Simple and sensitive method of microcantilever-based DNA detection using nanoparticles conjugates. In 3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS (pp. 160-163). [4484309] https://doi.org/10.1109/NEMS.2008.4484309

Simple and sensitive method of microcantilever-based DNA detection using nanoparticles conjugates. / Cha, Byung Hak; Lee, Sang Myung; Hwang, Kyo Seon; Kim, Sang Kyung; Lee, Yoon S.; Ju, Byeong Kwon; Kim, Tae Song.

3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS. 2008. p. 160-163 4484309.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Cha, BH, Lee, SM, Hwang, KS, Kim, SK, Lee, YS, Ju, BK & Kim, TS 2008, Simple and sensitive method of microcantilever-based DNA detection using nanoparticles conjugates. in 3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS., 4484309, pp. 160-163, 3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008, Sanya, China, 08/1/6. https://doi.org/10.1109/NEMS.2008.4484309
Cha BH, Lee SM, Hwang KS, Kim SK, Lee YS, Ju BK et al. Simple and sensitive method of microcantilever-based DNA detection using nanoparticles conjugates. In 3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS. 2008. p. 160-163. 4484309 https://doi.org/10.1109/NEMS.2008.4484309
Cha, Byung Hak ; Lee, Sang Myung ; Hwang, Kyo Seon ; Kim, Sang Kyung ; Lee, Yoon S. ; Ju, Byeong Kwon ; Kim, Tae Song. / Simple and sensitive method of microcantilever-based DNA detection using nanoparticles conjugates. 3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS. 2008. pp. 160-163
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