Simple fabrication method for a porous poly(vinyl alcohol) matrix by multisolvent mixtures for an air-exposed model of the lung epithelial system

Su Kyoung Chae, Cho Hay Mun, Da Yoon Noh, Edward Kang, Sang Hoon Lee

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We introduce a simple and easy method for fabricating a thin and porous matrix that can be used as an extracellular matrix (ECM). A porous poly(vinyl alcohol) (PVA) matrix was created through recrystallization by multiple solvents under distilled water (DW), isopropyl alcohol (IPA), and a combination of DW and IPA. The crysatllization was driven by precipitating and dissolving a solute in a solution of a solvent and a nonsolvent, which induced the formation of microspheres in the IPA. The crystal structure depended on the ratio of the solvent/nonsolvent and the concentration of the PVA aqueous solution; these properties were used to tune the thickness, size, and porosity of the matrices. The resulting PVA matrix was chemically stabilized through a reaction with glutaraldehyde in the IPA solution. We demonstrated that a very thin and porous PVA matrix provided an effective functional model of the lung epithelial system. Lung epithelial cells (A549) displayed a high affinity for this matrix, which was permeable to the culture medium. These properties facilitated culturing under the air environment.

Original languageEnglish
Pages (from-to)12107-12113
Number of pages7
JournalLangmuir
Volume30
Issue number41
DOIs
Publication statusPublished - 2014 Jan 1

Fingerprint

2-Propanol
lungs
alcohols
Alcohols
Air
isopropyl alcohol
Fabrication
Lung
fabrication
air
matrices
Water
Porosity
Glutaral
Microspheres
Extracellular Matrix
Culture Media
Crystal structure
Epithelial Cells
culture media

ASJC Scopus subject areas

  • Electrochemistry
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Materials Science(all)
  • Spectroscopy

Cite this

Simple fabrication method for a porous poly(vinyl alcohol) matrix by multisolvent mixtures for an air-exposed model of the lung epithelial system. / Chae, Su Kyoung; Mun, Cho Hay; Noh, Da Yoon; Kang, Edward; Lee, Sang Hoon.

In: Langmuir, Vol. 30, No. 41, 01.01.2014, p. 12107-12113.

Research output: Contribution to journalArticle

@article{5c565e8b96494f298ac0b72cef773198,
title = "Simple fabrication method for a porous poly(vinyl alcohol) matrix by multisolvent mixtures for an air-exposed model of the lung epithelial system",
abstract = "We introduce a simple and easy method for fabricating a thin and porous matrix that can be used as an extracellular matrix (ECM). A porous poly(vinyl alcohol) (PVA) matrix was created through recrystallization by multiple solvents under distilled water (DW), isopropyl alcohol (IPA), and a combination of DW and IPA. The crysatllization was driven by precipitating and dissolving a solute in a solution of a solvent and a nonsolvent, which induced the formation of microspheres in the IPA. The crystal structure depended on the ratio of the solvent/nonsolvent and the concentration of the PVA aqueous solution; these properties were used to tune the thickness, size, and porosity of the matrices. The resulting PVA matrix was chemically stabilized through a reaction with glutaraldehyde in the IPA solution. We demonstrated that a very thin and porous PVA matrix provided an effective functional model of the lung epithelial system. Lung epithelial cells (A549) displayed a high affinity for this matrix, which was permeable to the culture medium. These properties facilitated culturing under the air environment.",
author = "Chae, {Su Kyoung} and Mun, {Cho Hay} and Noh, {Da Yoon} and Edward Kang and Lee, {Sang Hoon}",
year = "2014",
month = "1",
day = "1",
doi = "10.1021/la501453h",
language = "English",
volume = "30",
pages = "12107--12113",
journal = "Langmuir",
issn = "0743-7463",
publisher = "American Chemical Society",
number = "41",

}

TY - JOUR

T1 - Simple fabrication method for a porous poly(vinyl alcohol) matrix by multisolvent mixtures for an air-exposed model of the lung epithelial system

AU - Chae, Su Kyoung

AU - Mun, Cho Hay

AU - Noh, Da Yoon

AU - Kang, Edward

AU - Lee, Sang Hoon

PY - 2014/1/1

Y1 - 2014/1/1

N2 - We introduce a simple and easy method for fabricating a thin and porous matrix that can be used as an extracellular matrix (ECM). A porous poly(vinyl alcohol) (PVA) matrix was created through recrystallization by multiple solvents under distilled water (DW), isopropyl alcohol (IPA), and a combination of DW and IPA. The crysatllization was driven by precipitating and dissolving a solute in a solution of a solvent and a nonsolvent, which induced the formation of microspheres in the IPA. The crystal structure depended on the ratio of the solvent/nonsolvent and the concentration of the PVA aqueous solution; these properties were used to tune the thickness, size, and porosity of the matrices. The resulting PVA matrix was chemically stabilized through a reaction with glutaraldehyde in the IPA solution. We demonstrated that a very thin and porous PVA matrix provided an effective functional model of the lung epithelial system. Lung epithelial cells (A549) displayed a high affinity for this matrix, which was permeable to the culture medium. These properties facilitated culturing under the air environment.

AB - We introduce a simple and easy method for fabricating a thin and porous matrix that can be used as an extracellular matrix (ECM). A porous poly(vinyl alcohol) (PVA) matrix was created through recrystallization by multiple solvents under distilled water (DW), isopropyl alcohol (IPA), and a combination of DW and IPA. The crysatllization was driven by precipitating and dissolving a solute in a solution of a solvent and a nonsolvent, which induced the formation of microspheres in the IPA. The crystal structure depended on the ratio of the solvent/nonsolvent and the concentration of the PVA aqueous solution; these properties were used to tune the thickness, size, and porosity of the matrices. The resulting PVA matrix was chemically stabilized through a reaction with glutaraldehyde in the IPA solution. We demonstrated that a very thin and porous PVA matrix provided an effective functional model of the lung epithelial system. Lung epithelial cells (A549) displayed a high affinity for this matrix, which was permeable to the culture medium. These properties facilitated culturing under the air environment.

UR - http://www.scopus.com/inward/record.url?scp=84908072322&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84908072322&partnerID=8YFLogxK

U2 - 10.1021/la501453h

DO - 10.1021/la501453h

M3 - Article

C2 - 25260012

AN - SCOPUS:84908072322

VL - 30

SP - 12107

EP - 12113

JO - Langmuir

JF - Langmuir

SN - 0743-7463

IS - 41

ER -