Simple fabrication method for a porous poly(vinyl alcohol) matrix by multisolvent mixtures for an air-exposed model of the lung epithelial system

Su Kyoung Chae, Cho Hay Mun, Da Yoon Noh, Edward Kang, Sang Hoon Lee

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We introduce a simple and easy method for fabricating a thin and porous matrix that can be used as an extracellular matrix (ECM). A porous poly(vinyl alcohol) (PVA) matrix was created through recrystallization by multiple solvents under distilled water (DW), isopropyl alcohol (IPA), and a combination of DW and IPA. The crysatllization was driven by precipitating and dissolving a solute in a solution of a solvent and a nonsolvent, which induced the formation of microspheres in the IPA. The crystal structure depended on the ratio of the solvent/nonsolvent and the concentration of the PVA aqueous solution; these properties were used to tune the thickness, size, and porosity of the matrices. The resulting PVA matrix was chemically stabilized through a reaction with glutaraldehyde in the IPA solution. We demonstrated that a very thin and porous PVA matrix provided an effective functional model of the lung epithelial system. Lung epithelial cells (A549) displayed a high affinity for this matrix, which was permeable to the culture medium. These properties facilitated culturing under the air environment.

Original languageEnglish
Pages (from-to)12107-12113
Number of pages7
JournalLangmuir
Volume30
Issue number41
DOIs
Publication statusPublished - 2014 Jan 1

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ASJC Scopus subject areas

  • Electrochemistry
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Materials Science(all)
  • Spectroscopy

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