Simple methods for tracking stem cells with <sup>64</sup>Cu-labeled DOTA-hexadecyl-benzoate

Min Hwan Kim, Sang Keun Woo, Kwang Il Kim, Tae Sup Lee, Chan Wha Kim, Joo Hyun Kang, Byung Il Kim, Sang Moo Lim, Kyo Chul Lee, Yong Jin Lee

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The purpose of this study was to evaluate <sup>64</sup>Cu-labeled hexadecyl-1,4,7,10-tetraazacyclododecane-tetraacetic acid-benzoate (<sup>64</sup>Cu-DOTA-HB) (1) as positron emission tomography (PET) radiotracer for stem cell imaging. Hexadecyl-DOTA-benzoate (DOTA-HB) (2) was efficiently labeled with <sup>64</sup>Cu (>99%), and cell labeling efficiency with adipose-derived stem cells (ADSCs) was over 50%. Labeling with 1 did not compromise cell viability. In the PET imaging, intramuscularly transplanted 1-labeled ADSCs were monitored for 18 h in normal rat heart. These results indicate that 1 can be utilized as a promising radiotracer for monitoring of transplanted stem cells.

Original languageEnglish
Pages (from-to)528-530
Number of pages3
JournalACS Medicinal Chemistry Letters
Volume6
Issue number5
DOIs
Publication statusPublished - 2015 May 14

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Keywords

  • Adipose-derived stem cells
  • copper-64
  • hexadecyl benzoate
  • PET
  • radiotracer

ASJC Scopus subject areas

  • Organic Chemistry
  • Drug Discovery
  • Biochemistry

Cite this

Kim, M. H., Woo, S. K., Kim, K. I., Lee, T. S., Kim, C. W., Kang, J. H., Kim, B. I., Lim, S. M., Lee, K. C., & Lee, Y. J. (2015). Simple methods for tracking stem cells with <sup>64</sup>Cu-labeled DOTA-hexadecyl-benzoate. ACS Medicinal Chemistry Letters, 6(5), 528-530. https://doi.org/10.1021/acsmedchemlett.5b00021