Simple surface biofunctionalization of biphasic calcium phosphates for improving osteogenic activity and bone tissue regeneration

Kyu Sik Shim; Hak Jun Kim; Sung Eum Kim; Kyeongsoon Park

doi:10.1016/j.jiec.2018.07.048

Simple surface biofunctionalization of biphasic calcium phosphates for improving osteogenic activity and bone tissue regeneration

Kyu Sik Shim, Hak Jun Kim, Sung Eum Kim, Kyeongsoon Park

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

In this study, we found a simple surface biofunctionalization of biphasic calcium phosphate (BCP) based on the high affinity between alendronate and the calcium ions of BCP, and the strong interaction between heparin and bone morphogenic protein-2 (BMP-2). The biofunctionalized BCP did not be precipitated well and display a remarkable enhancement of osteogenic activity of human adipose-derived stem cells by showing increased alkaline phosphatase (ALP), calcium deposition and osteogenic-related genes (i.e., Runx-2, ALP, osteocalcin, and osteopontin), and bone regeneration in the calvarial defect model. Therefore, this simple surface technique can be used to easily functionalize various calcium phosphates.

Original language	English
Pages (from-to)	220-228
Number of pages	9
Journal	Journal of Industrial and Engineering Chemistry
Volume	68
DOIs	https://doi.org/10.1016/j.jiec.2018.07.048
Publication status	Published - 2018 Dec 25

Keywords

Alendronate
Biphasic calcium phosphate
Heparin
Osteogenesis
Surface biofunctionalization

ASJC Scopus subject areas

Chemical Engineering(all)

Access to Document

10.1016/j.jiec.2018.07.048

Cite this

@article{d113dfe2543440b79e398fa72640ab56,

title = "Simple surface biofunctionalization of biphasic calcium phosphates for improving osteogenic activity and bone tissue regeneration",

abstract = "In this study, we found a simple surface biofunctionalization of biphasic calcium phosphate (BCP) based on the high affinity between alendronate and the calcium ions of BCP, and the strong interaction between heparin and bone morphogenic protein-2 (BMP-2). The biofunctionalized BCP did not be precipitated well and display a remarkable enhancement of osteogenic activity of human adipose-derived stem cells by showing increased alkaline phosphatase (ALP), calcium deposition and osteogenic-related genes (i.e., Runx-2, ALP, osteocalcin, and osteopontin), and bone regeneration in the calvarial defect model. Therefore, this simple surface technique can be used to easily functionalize various calcium phosphates.",

keywords = "Alendronate, Biphasic calcium phosphate, Heparin, Osteogenesis, Surface biofunctionalization",

author = "Shim, {Kyu Sik} and Kim, {Hak Jun} and Kim, {Sung Eum} and Kyeongsoon Park",

note = "Funding Information: This research was supported by the Bio & Medical Technology Development Program of the NRF funded by the Korean government, MSIP (NRF-2017M3A9B3063640) and by the Chung-Ang University Research Grants in 2017. Funding Information: This research was supported by the Bio & Medical Technology Development Program of the NRF funded by the Korean government , MSIP (NRF- 2017M3A9B3063640 ) and by the Chung-Ang University Research Grants in 2017. Publisher Copyright: {\textcopyright} 2018 The Korean Society of Industrial and Engineering Chemistry",

year = "2018",

month = dec,

day = "25",

doi = "10.1016/j.jiec.2018.07.048",

language = "English",

volume = "68",

pages = "220--228",

journal = "Journal of Industrial and Engineering Chemistry",

issn = "1226-086X",

publisher = "Korean Society of Industrial Engineering Chemistry",

}

TY - JOUR

T1 - Simple surface biofunctionalization of biphasic calcium phosphates for improving osteogenic activity and bone tissue regeneration

AU - Shim, Kyu Sik

AU - Kim, Hak Jun

AU - Kim, Sung Eum

AU - Park, Kyeongsoon

N1 - Funding Information: This research was supported by the Bio & Medical Technology Development Program of the NRF funded by the Korean government, MSIP (NRF-2017M3A9B3063640) and by the Chung-Ang University Research Grants in 2017. Funding Information: This research was supported by the Bio & Medical Technology Development Program of the NRF funded by the Korean government , MSIP (NRF- 2017M3A9B3063640 ) and by the Chung-Ang University Research Grants in 2017. Publisher Copyright: © 2018 The Korean Society of Industrial and Engineering Chemistry

PY - 2018/12/25

Y1 - 2018/12/25

N2 - In this study, we found a simple surface biofunctionalization of biphasic calcium phosphate (BCP) based on the high affinity between alendronate and the calcium ions of BCP, and the strong interaction between heparin and bone morphogenic protein-2 (BMP-2). The biofunctionalized BCP did not be precipitated well and display a remarkable enhancement of osteogenic activity of human adipose-derived stem cells by showing increased alkaline phosphatase (ALP), calcium deposition and osteogenic-related genes (i.e., Runx-2, ALP, osteocalcin, and osteopontin), and bone regeneration in the calvarial defect model. Therefore, this simple surface technique can be used to easily functionalize various calcium phosphates.

AB - In this study, we found a simple surface biofunctionalization of biphasic calcium phosphate (BCP) based on the high affinity between alendronate and the calcium ions of BCP, and the strong interaction between heparin and bone morphogenic protein-2 (BMP-2). The biofunctionalized BCP did not be precipitated well and display a remarkable enhancement of osteogenic activity of human adipose-derived stem cells by showing increased alkaline phosphatase (ALP), calcium deposition and osteogenic-related genes (i.e., Runx-2, ALP, osteocalcin, and osteopontin), and bone regeneration in the calvarial defect model. Therefore, this simple surface technique can be used to easily functionalize various calcium phosphates.

KW - Alendronate

KW - Biphasic calcium phosphate

KW - Heparin

KW - Osteogenesis

KW - Surface biofunctionalization

UR - http://www.scopus.com/inward/record.url?scp=85051048125&partnerID=8YFLogxK

U2 - 10.1016/j.jiec.2018.07.048

DO - 10.1016/j.jiec.2018.07.048

M3 - Article

AN - SCOPUS:85051048125

VL - 68

SP - 220

EP - 228

JO - Journal of Industrial and Engineering Chemistry

JF - Journal of Industrial and Engineering Chemistry

SN - 1226-086X

ER -

Simple surface biofunctionalization of biphasic calcium phosphates for improving osteogenic activity and bone tissue regeneration

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this