Simple surface biofunctionalization of biphasic calcium phosphates for improving osteogenic activity and bone tissue regeneration

Kyu Sik Shim, Hak Jun Kim, Sung Eum Kim, Kyeongsoon Park

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In this study, we found a simple surface biofunctionalization of biphasic calcium phosphate (BCP) based on the high affinity between alendronate and the calcium ions of BCP, and the strong interaction between heparin and bone morphogenic protein-2 (BMP-2). The biofunctionalized BCP did not be precipitated well and display a remarkable enhancement of osteogenic activity of human adipose-derived stem cells by showing increased alkaline phosphatase (ALP), calcium deposition and osteogenic-related genes (i.e., Runx-2, ALP, osteocalcin, and osteopontin), and bone regeneration in the calvarial defect model. Therefore, this simple surface technique can be used to easily functionalize various calcium phosphates.

Original languageEnglish
JournalJournal of Industrial and Engineering Chemistry
DOIs
Publication statusAccepted/In press - 2018 Jan 1

Fingerprint

Tissue regeneration
Calcium phosphate
Bone
Alkaline Phosphatase
Phosphatases
Calcium Phosphates
Calcium
Alendronate
Osteopontin
Osteocalcin
Stem cells
Heparin
Genes
Ions
Defects
Proteins
hydroxyapatite-beta tricalcium phosphate

Keywords

  • Alendronate
  • Biphasic calcium phosphate
  • Heparin
  • Osteogenesis
  • Surface biofunctionalization

ASJC Scopus subject areas

  • Chemical Engineering(all)

Cite this

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title = "Simple surface biofunctionalization of biphasic calcium phosphates for improving osteogenic activity and bone tissue regeneration",
abstract = "In this study, we found a simple surface biofunctionalization of biphasic calcium phosphate (BCP) based on the high affinity between alendronate and the calcium ions of BCP, and the strong interaction between heparin and bone morphogenic protein-2 (BMP-2). The biofunctionalized BCP did not be precipitated well and display a remarkable enhancement of osteogenic activity of human adipose-derived stem cells by showing increased alkaline phosphatase (ALP), calcium deposition and osteogenic-related genes (i.e., Runx-2, ALP, osteocalcin, and osteopontin), and bone regeneration in the calvarial defect model. Therefore, this simple surface technique can be used to easily functionalize various calcium phosphates.",
keywords = "Alendronate, Biphasic calcium phosphate, Heparin, Osteogenesis, Surface biofunctionalization",
author = "Shim, {Kyu Sik} and Kim, {Hak Jun} and Kim, {Sung Eum} and Kyeongsoon Park",
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T1 - Simple surface biofunctionalization of biphasic calcium phosphates for improving osteogenic activity and bone tissue regeneration

AU - Shim, Kyu Sik

AU - Kim, Hak Jun

AU - Kim, Sung Eum

AU - Park, Kyeongsoon

PY - 2018/1/1

Y1 - 2018/1/1

N2 - In this study, we found a simple surface biofunctionalization of biphasic calcium phosphate (BCP) based on the high affinity between alendronate and the calcium ions of BCP, and the strong interaction between heparin and bone morphogenic protein-2 (BMP-2). The biofunctionalized BCP did not be precipitated well and display a remarkable enhancement of osteogenic activity of human adipose-derived stem cells by showing increased alkaline phosphatase (ALP), calcium deposition and osteogenic-related genes (i.e., Runx-2, ALP, osteocalcin, and osteopontin), and bone regeneration in the calvarial defect model. Therefore, this simple surface technique can be used to easily functionalize various calcium phosphates.

AB - In this study, we found a simple surface biofunctionalization of biphasic calcium phosphate (BCP) based on the high affinity between alendronate and the calcium ions of BCP, and the strong interaction between heparin and bone morphogenic protein-2 (BMP-2). The biofunctionalized BCP did not be precipitated well and display a remarkable enhancement of osteogenic activity of human adipose-derived stem cells by showing increased alkaline phosphatase (ALP), calcium deposition and osteogenic-related genes (i.e., Runx-2, ALP, osteocalcin, and osteopontin), and bone regeneration in the calvarial defect model. Therefore, this simple surface technique can be used to easily functionalize various calcium phosphates.

KW - Alendronate

KW - Biphasic calcium phosphate

KW - Heparin

KW - Osteogenesis

KW - Surface biofunctionalization

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