Abstract
Uniformly sized silica-coated magnetic nanoparticles (magnetite@silica) are synthesized in a simple one-pot process using reverse micelles as nanoreactors. The core diameter of the magnetic nanoparticles is easily controlled by adjusting the w value ([polar solvent]/[surfactant]) in the reverse-micelle solution, and the thickness of the silica shell is easily controlled by varying the amount of tetraethyl orthosilicate added after the synthesis of the magnetite cores. Several grams of monodisperse magnetite@silica nanoparticles can be synthesized without going through any size-selection process. When crosslinked enzyme molecules form clusters on the surfaces of the magnetite@silica nanoparticles, the resulting hybrid composites are magnetically separable, highly active, and stable under harsh shaking conditions for more than 15 days. Conversely, covalently attached enzymes on the surface of the magnetite@silica nanoparticles are deactivated under the same conditions.
Original language | English |
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Pages (from-to) | 143-152 |
Number of pages | 10 |
Journal | Small |
Volume | 4 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2008 Jan |
Externally published | Yes |
Keywords
- Core/shell materials
- Iron oxides
- Silica
- Superparamagnetism
- Surface functionalization
ASJC Scopus subject areas
- Biotechnology
- Biomaterials
- Chemistry(all)
- Materials Science(all)