Abstract
Uniformly sized silica-coated magnetic nanoparticles (magnetite@silica) are synthesized in a simple one-pot process using reverse micelles as nanoreactors. The core diameter of the magnetic nanoparticles is easily controlled by adjusting the w value ([polar solvent]/[surfactant]) in the reverse-micelle solution, and the thickness of the silica shell is easily controlled by varying the amount of tetraethyl orthosilicate added after the synthesis of the magnetite cores. Several grams of monodisperse magnetite@silica nanoparticles can be synthesized without going through any size-selection process. When crosslinked enzyme molecules form clusters on the surfaces of the magnetite@silica nanoparticles, the resulting hybrid composites are magnetically separable, highly active, and stable under harsh shaking conditions for more than 15 days. Conversely, covalently attached enzymes on the surface of the magnetite@silica nanoparticles are deactivated under the same conditions.
| Original language | English |
|---|---|
| Pages (from-to) | 143-152 |
| Number of pages | 10 |
| Journal | Small |
| Volume | 4 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2008 Jan 1 |
| Externally published | Yes |
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Keywords
- Core/shell materials
- Iron oxides
- Silica
- Superparamagnetism
- Surface functionalization
ASJC Scopus subject areas
- Biomaterials
- Engineering (miscellaneous)
- Biotechnology
- Medicine(all)
Cite this
Simple synthesis of functionalized superparamagnetic magnetite/silica core/shell nanoparticles and their application as magnetically separable high-performance biocatalysts. / Lee, Jinwoo; Lee, Youjin; Youn, Jong Kyu; Na, Hyon Bin; Yu, Taekyung; Kim, Hwan; Lee, Sang Mok; Koo, Yoon Mo; Kwak, Ja Hun; Park, Hyun Gyu; Chang, Ho Nam; Hwang, Misun; Park, Je Geun; Kim, Jungbae; Hyeon, Taeghwan.
In: Small, Vol. 4, No. 1, 01.01.2008, p. 143-152.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Simple synthesis of functionalized superparamagnetic magnetite/silica core/shell nanoparticles and their application as magnetically separable high-performance biocatalysts
AU - Lee, Jinwoo
AU - Lee, Youjin
AU - Youn, Jong Kyu
AU - Na, Hyon Bin
AU - Yu, Taekyung
AU - Kim, Hwan
AU - Lee, Sang Mok
AU - Koo, Yoon Mo
AU - Kwak, Ja Hun
AU - Park, Hyun Gyu
AU - Chang, Ho Nam
AU - Hwang, Misun
AU - Park, Je Geun
AU - Kim, Jungbae
AU - Hyeon, Taeghwan
PY - 2008/1/1
Y1 - 2008/1/1
N2 - Uniformly sized silica-coated magnetic nanoparticles (magnetite@silica) are synthesized in a simple one-pot process using reverse micelles as nanoreactors. The core diameter of the magnetic nanoparticles is easily controlled by adjusting the w value ([polar solvent]/[surfactant]) in the reverse-micelle solution, and the thickness of the silica shell is easily controlled by varying the amount of tetraethyl orthosilicate added after the synthesis of the magnetite cores. Several grams of monodisperse magnetite@silica nanoparticles can be synthesized without going through any size-selection process. When crosslinked enzyme molecules form clusters on the surfaces of the magnetite@silica nanoparticles, the resulting hybrid composites are magnetically separable, highly active, and stable under harsh shaking conditions for more than 15 days. Conversely, covalently attached enzymes on the surface of the magnetite@silica nanoparticles are deactivated under the same conditions.
AB - Uniformly sized silica-coated magnetic nanoparticles (magnetite@silica) are synthesized in a simple one-pot process using reverse micelles as nanoreactors. The core diameter of the magnetic nanoparticles is easily controlled by adjusting the w value ([polar solvent]/[surfactant]) in the reverse-micelle solution, and the thickness of the silica shell is easily controlled by varying the amount of tetraethyl orthosilicate added after the synthesis of the magnetite cores. Several grams of monodisperse magnetite@silica nanoparticles can be synthesized without going through any size-selection process. When crosslinked enzyme molecules form clusters on the surfaces of the magnetite@silica nanoparticles, the resulting hybrid composites are magnetically separable, highly active, and stable under harsh shaking conditions for more than 15 days. Conversely, covalently attached enzymes on the surface of the magnetite@silica nanoparticles are deactivated under the same conditions.
KW - Core/shell materials
KW - Iron oxides
KW - Silica
KW - Superparamagnetism
KW - Surface functionalization
UR - http://www.scopus.com/inward/record.url?scp=38849162786&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=38849162786&partnerID=8YFLogxK
U2 - 10.1002/smll.200700456
DO - 10.1002/smll.200700456
M3 - Article
VL - 4
SP - 143
EP - 152
JO - Small
JF - Small
SN - 1613-6810
IS - 1
ER -