Simple synthesis of functionalized superparamagnetic magnetite/silica core/shell nanoparticles and their application as magnetically separable high-performance biocatalysts

Jinwoo Lee, Youjin Lee, Jong Kyu Youn, Hyon Bin Na, Taekyung Yu, Hwan Kim, Sang Mok Lee, Yoon Mo Koo, Ja Hun Kwak, Hyun Gyu Park, Ho Nam Chang, Misun Hwang, Je Geun Park, Jungbae Kim, Taeghwan Hyeon

Research output: Contribution to journalArticle

322 Citations (Scopus)

Abstract

Uniformly sized silica-coated magnetic nanoparticles (magnetite@silica) are synthesized in a simple one-pot process using reverse micelles as nanoreactors. The core diameter of the magnetic nanoparticles is easily controlled by adjusting the w value ([polar solvent]/[surfactant]) in the reverse-micelle solution, and the thickness of the silica shell is easily controlled by varying the amount of tetraethyl orthosilicate added after the synthesis of the magnetite cores. Several grams of monodisperse magnetite@silica nanoparticles can be synthesized without going through any size-selection process. When crosslinked enzyme molecules form clusters on the surfaces of the magnetite@silica nanoparticles, the resulting hybrid composites are magnetically separable, highly active, and stable under harsh shaking conditions for more than 15 days. Conversely, covalently attached enzymes on the surface of the magnetite@silica nanoparticles are deactivated under the same conditions.

Original languageEnglish
Pages (from-to)143-152
Number of pages10
JournalSmall
Volume4
Issue number1
DOIs
Publication statusPublished - 2008 Jan

Keywords

  • Core/shell materials
  • Iron oxides
  • Silica
  • Superparamagnetism
  • Surface functionalization

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Chemistry(all)
  • Materials Science(all)

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  • Cite this

    Lee, J., Lee, Y., Youn, J. K., Na, H. B., Yu, T., Kim, H., Lee, S. M., Koo, Y. M., Kwak, J. H., Park, H. G., Chang, H. N., Hwang, M., Park, J. G., Kim, J., & Hyeon, T. (2008). Simple synthesis of functionalized superparamagnetic magnetite/silica core/shell nanoparticles and their application as magnetically separable high-performance biocatalysts. Small, 4(1), 143-152. https://doi.org/10.1002/smll.200700456