Abstract
A Gram-negative, motile, rod-shaped bacterium, designated strain EMB325T was isolated from activated sludge that performed enhanced biological phosphorus a removal in a sequencing batch reactor. The predominant fatty acids of strain EMB325T were summed feature 3 (C18::1ω7c and/ or ISO-C15:0 2-OH), C16:0, C18:1ω7c and C18:1w7c 11-methyl. The strain contained phosphatidylethanolamine and diphosphatidylglycerol as polar lipids. The G + C content of the genomic DNA was 63.3 mol%. The major quinone was Q-8. Phylogenetic analysis of 16S rRNA gene sequences showed that strain EMB325T formed a phyletic lineage with members of the genus Simplicispira and was most closely related to Simplicispira psychrophila LMG 5408T and Simplicispira metamorpha DSM 1837T with similarities of 98.1 and 97.9%, respectively. Levels of DNA-DNA relatedness between strain EMB325T and S. psychrophila LMG 5408T and S. metamorpha DSM 1837T were 28 and 23 %, respectively. On the basis of chernotaxonomic data and molecular characteristics, strain EMB325T is considered to rpresent a novel species within the genus Simplicispira, for which the name Simplicispira limi sp. nov. is proposed. The type strain is the EMB325T (= KCTC 12608T = DSM 17964T).
| Original language | English |
|---|---|
| Pages (from-to) | 31-34 |
| Number of pages | 4 |
| Journal | International Journal of Systematic and Evolutionary Microbiology |
| Volume | 57 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2007 Jan 1 |
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ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Applied Microbiology and Biotechnology
- Immunology
- Microbiology
Cite this
Simplicispira limi sp. nov., isolated from activated sludge. / Lu, Shipeng; Ryu, Seung Hyun; Chung, Bok Sil; Chung, Young Ryun; Park, Woojun; Jeon, Che O.
In: International Journal of Systematic and Evolutionary Microbiology, Vol. 57, No. 1, 01.01.2007, p. 31-34.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Simplicispira limi sp. nov., isolated from activated sludge
AU - Lu, Shipeng
AU - Ryu, Seung Hyun
AU - Chung, Bok Sil
AU - Chung, Young Ryun
AU - Park, Woojun
AU - Jeon, Che O.
PY - 2007/1/1
Y1 - 2007/1/1
N2 - A Gram-negative, motile, rod-shaped bacterium, designated strain EMB325T was isolated from activated sludge that performed enhanced biological phosphorus a removal in a sequencing batch reactor. The predominant fatty acids of strain EMB325T were summed feature 3 (C18::1ω7c and/ or ISO-C15:0 2-OH), C16:0, C18:1ω7c and C18:1w7c 11-methyl. The strain contained phosphatidylethanolamine and diphosphatidylglycerol as polar lipids. The G + C content of the genomic DNA was 63.3 mol%. The major quinone was Q-8. Phylogenetic analysis of 16S rRNA gene sequences showed that strain EMB325T formed a phyletic lineage with members of the genus Simplicispira and was most closely related to Simplicispira psychrophila LMG 5408T and Simplicispira metamorpha DSM 1837T with similarities of 98.1 and 97.9%, respectively. Levels of DNA-DNA relatedness between strain EMB325T and S. psychrophila LMG 5408T and S. metamorpha DSM 1837T were 28 and 23 %, respectively. On the basis of chernotaxonomic data and molecular characteristics, strain EMB325T is considered to rpresent a novel species within the genus Simplicispira, for which the name Simplicispira limi sp. nov. is proposed. The type strain is the EMB325T (= KCTC 12608T = DSM 17964T).
AB - A Gram-negative, motile, rod-shaped bacterium, designated strain EMB325T was isolated from activated sludge that performed enhanced biological phosphorus a removal in a sequencing batch reactor. The predominant fatty acids of strain EMB325T were summed feature 3 (C18::1ω7c and/ or ISO-C15:0 2-OH), C16:0, C18:1ω7c and C18:1w7c 11-methyl. The strain contained phosphatidylethanolamine and diphosphatidylglycerol as polar lipids. The G + C content of the genomic DNA was 63.3 mol%. The major quinone was Q-8. Phylogenetic analysis of 16S rRNA gene sequences showed that strain EMB325T formed a phyletic lineage with members of the genus Simplicispira and was most closely related to Simplicispira psychrophila LMG 5408T and Simplicispira metamorpha DSM 1837T with similarities of 98.1 and 97.9%, respectively. Levels of DNA-DNA relatedness between strain EMB325T and S. psychrophila LMG 5408T and S. metamorpha DSM 1837T were 28 and 23 %, respectively. On the basis of chernotaxonomic data and molecular characteristics, strain EMB325T is considered to rpresent a novel species within the genus Simplicispira, for which the name Simplicispira limi sp. nov. is proposed. The type strain is the EMB325T (= KCTC 12608T = DSM 17964T).
UR - http://www.scopus.com/inward/record.url?scp=33846500076&partnerID=8YFLogxK
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U2 - 10.1099/ijs.0.64566-0
DO - 10.1099/ijs.0.64566-0
M3 - Article
VL - 57
SP - 31
EP - 34
JO - The BMJ
JF - The BMJ
SN - 0730-6512
IS - 1
ER -