Simplicispira limi sp. nov., isolated from activated sludge

Shipeng Lu; Seung Hyun Ryu; Bok Sil Chung; Young Ryun Chung; Woojun Park; Che Ok Jeon

doi:10.1099/ijs.0.64566-0

Simplicispira limi sp. nov., isolated from activated sludge

Shipeng Lu, Seung Hyun Ryu, Bok Sil Chung, Young Ryun Chung, Woojun Park, Che Ok Jeon

Division of Environmental Science and Ecological Engineering

Research output: Contribution to journal › Article › peer-review

51 Citations (Scopus)

Abstract

A Gram-negative, motile, rod-shaped bacterium, designated strain EMB325^T was isolated from activated sludge that performed enhanced biological phosphorus a removal in a sequencing batch reactor. The predominant fatty acids of strain EMB325^T were summed feature 3 (C₁₈::_1ω7c and/ or ISO-C_15:0 2-OH), C_16:0, C_18:1ω7c and C_18:1w7c 11-methyl. The strain contained phosphatidylethanolamine and diphosphatidylglycerol as polar lipids. The G + C content of the genomic DNA was 63.3 mol%. The major quinone was Q-8. Phylogenetic analysis of 16S rRNA gene sequences showed that strain EMB325^T formed a phyletic lineage with members of the genus Simplicispira and was most closely related to Simplicispira psychrophila LMG 5408^T and Simplicispira metamorpha DSM 1837^T with similarities of 98.1 and 97.9%, respectively. Levels of DNA-DNA relatedness between strain EMB325^T and S. psychrophila LMG 5408^T and S. metamorpha DSM 1837^T were 28 and 23 %, respectively. On the basis of chernotaxonomic data and molecular characteristics, strain EMB325^T is considered to rpresent a novel species within the genus Simplicispira, for which the name Simplicispira limi sp. nov. is proposed. The type strain is the EMB325^T (= KCTC 12608^T = DSM 17964^T).

Original language	English
Pages (from-to)	31-34
Number of pages	4
Journal	International Journal of Systematic and Evolutionary Microbiology
Volume	57
Issue number	1
DOIs	https://doi.org/10.1099/ijs.0.64566-0
Publication status	Published - 2007 Jan

ASJC Scopus subject areas

Microbiology
Ecology, Evolution, Behavior and Systematics

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title = "Simplicispira limi sp. nov., isolated from activated sludge",

abstract = "A Gram-negative, motile, rod-shaped bacterium, designated strain EMB325T was isolated from activated sludge that performed enhanced biological phosphorus a removal in a sequencing batch reactor. The predominant fatty acids of strain EMB325T were summed feature 3 (C18::1ω7c and/ or ISO-C15:0 2-OH), C16:0, C18:1ω7c and C18:1w7c 11-methyl. The strain contained phosphatidylethanolamine and diphosphatidylglycerol as polar lipids. The G + C content of the genomic DNA was 63.3 mol%. The major quinone was Q-8. Phylogenetic analysis of 16S rRNA gene sequences showed that strain EMB325T formed a phyletic lineage with members of the genus Simplicispira and was most closely related to Simplicispira psychrophila LMG 5408T and Simplicispira metamorpha DSM 1837T with similarities of 98.1 and 97.9%, respectively. Levels of DNA-DNA relatedness between strain EMB325T and S. psychrophila LMG 5408T and S. metamorpha DSM 1837T were 28 and 23 %, respectively. On the basis of chernotaxonomic data and molecular characteristics, strain EMB325T is considered to rpresent a novel species within the genus Simplicispira, for which the name Simplicispira limi sp. nov. is proposed. The type strain is the EMB325T (= KCTC 12608T = DSM 17964T).",

author = "Shipeng Lu and Ryu, {Seung Hyun} and Chung, {Bok Sil} and Chung, {Young Ryun} and Woojun Park and Jeon, {Che Ok}",

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doi = "10.1099/ijs.0.64566-0",

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T1 - Simplicispira limi sp. nov., isolated from activated sludge

AU - Lu, Shipeng

AU - Ryu, Seung Hyun

AU - Chung, Bok Sil

AU - Chung, Young Ryun

AU - Park, Woojun

AU - Jeon, Che Ok

PY - 2007/1

Y1 - 2007/1

N2 - A Gram-negative, motile, rod-shaped bacterium, designated strain EMB325T was isolated from activated sludge that performed enhanced biological phosphorus a removal in a sequencing batch reactor. The predominant fatty acids of strain EMB325T were summed feature 3 (C18::1ω7c and/ or ISO-C15:0 2-OH), C16:0, C18:1ω7c and C18:1w7c 11-methyl. The strain contained phosphatidylethanolamine and diphosphatidylglycerol as polar lipids. The G + C content of the genomic DNA was 63.3 mol%. The major quinone was Q-8. Phylogenetic analysis of 16S rRNA gene sequences showed that strain EMB325T formed a phyletic lineage with members of the genus Simplicispira and was most closely related to Simplicispira psychrophila LMG 5408T and Simplicispira metamorpha DSM 1837T with similarities of 98.1 and 97.9%, respectively. Levels of DNA-DNA relatedness between strain EMB325T and S. psychrophila LMG 5408T and S. metamorpha DSM 1837T were 28 and 23 %, respectively. On the basis of chernotaxonomic data and molecular characteristics, strain EMB325T is considered to rpresent a novel species within the genus Simplicispira, for which the name Simplicispira limi sp. nov. is proposed. The type strain is the EMB325T (= KCTC 12608T = DSM 17964T).

AB - A Gram-negative, motile, rod-shaped bacterium, designated strain EMB325T was isolated from activated sludge that performed enhanced biological phosphorus a removal in a sequencing batch reactor. The predominant fatty acids of strain EMB325T were summed feature 3 (C18::1ω7c and/ or ISO-C15:0 2-OH), C16:0, C18:1ω7c and C18:1w7c 11-methyl. The strain contained phosphatidylethanolamine and diphosphatidylglycerol as polar lipids. The G + C content of the genomic DNA was 63.3 mol%. The major quinone was Q-8. Phylogenetic analysis of 16S rRNA gene sequences showed that strain EMB325T formed a phyletic lineage with members of the genus Simplicispira and was most closely related to Simplicispira psychrophila LMG 5408T and Simplicispira metamorpha DSM 1837T with similarities of 98.1 and 97.9%, respectively. Levels of DNA-DNA relatedness between strain EMB325T and S. psychrophila LMG 5408T and S. metamorpha DSM 1837T were 28 and 23 %, respectively. On the basis of chernotaxonomic data and molecular characteristics, strain EMB325T is considered to rpresent a novel species within the genus Simplicispira, for which the name Simplicispira limi sp. nov. is proposed. The type strain is the EMB325T (= KCTC 12608T = DSM 17964T).

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Simplicispira limi sp. nov., isolated from activated sludge

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