Simplicispira limi sp. nov., isolated from activated sludge

Shipeng Lu, Seung Hyun Ryu, Bok Sil Chung, Young Ryun Chung, Woojun Park, Che O. Jeon

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

A Gram-negative, motile, rod-shaped bacterium, designated strain EMB325T was isolated from activated sludge that performed enhanced biological phosphorus a removal in a sequencing batch reactor. The predominant fatty acids of strain EMB325T were summed feature 3 (C18::7c and/ or ISO-C15:0 2-OH), C16:0, C18:1ω7c and C18:1w7c 11-methyl. The strain contained phosphatidylethanolamine and diphosphatidylglycerol as polar lipids. The G + C content of the genomic DNA was 63.3 mol%. The major quinone was Q-8. Phylogenetic analysis of 16S rRNA gene sequences showed that strain EMB325T formed a phyletic lineage with members of the genus Simplicispira and was most closely related to Simplicispira psychrophila LMG 5408T and Simplicispira metamorpha DSM 1837T with similarities of 98.1 and 97.9%, respectively. Levels of DNA-DNA relatedness between strain EMB325T and S. psychrophila LMG 5408T and S. metamorpha DSM 1837T were 28 and 23 %, respectively. On the basis of chernotaxonomic data and molecular characteristics, strain EMB325T is considered to rpresent a novel species within the genus Simplicispira, for which the name Simplicispira limi sp. nov. is proposed. The type strain is the EMB325T (= KCTC 12608T = DSM 17964T).

Original languageEnglish
Pages (from-to)31-34
Number of pages4
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume57
Issue number1
DOIs
Publication statusPublished - 2007 Jan 1

Fingerprint

activated sludge
Sewage
DNA
new species
Cardiolipins
Base Composition
rRNA Genes
relatedness
Phosphorus
Names
genomics
Fatty Acids
fatty acid
lipid
phosphorus
Bacteria
phylogenetics
Lipids
bacterium
gene

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Applied Microbiology and Biotechnology
  • Immunology
  • Microbiology

Cite this

Simplicispira limi sp. nov., isolated from activated sludge. / Lu, Shipeng; Ryu, Seung Hyun; Chung, Bok Sil; Chung, Young Ryun; Park, Woojun; Jeon, Che O.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 57, No. 1, 01.01.2007, p. 31-34.

Research output: Contribution to journalArticle

Lu, Shipeng ; Ryu, Seung Hyun ; Chung, Bok Sil ; Chung, Young Ryun ; Park, Woojun ; Jeon, Che O. / Simplicispira limi sp. nov., isolated from activated sludge. In: International Journal of Systematic and Evolutionary Microbiology. 2007 ; Vol. 57, No. 1. pp. 31-34.
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