Simulated microgravity effects on nonsmall cell lung cancer cell proliferation and migration

Jae Ho Chung, Chi Bum Ahn, Kuk Hui Son, Eunjue Yi, Ho Sung Son, Han Sung Kim, Sung Ho Lee

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

BACKGROUND: Despite improvements in medical technology, lung cancer metastasis remains a global health problem. The effects of microgravity on cell morphology, structure, functions, and their mechanisms have been widely studied however, the biological effects of simulated microgravity on the interaction between cells and its eventual influence on the characteristics of cancer cells are yet to be discovered. We examined the effects of simulated microgravity on the metastatic ability of different lung cancer cells using a random positioning machine. METHODS: Human lung cancer cell lines of adenocarcinoma (A549) and squamous cell carcinoma (H1703) were cultured in a 3D clinostat system which was continuously rotated at 5 rpm for 36 h. The experimental and control group were cultured under identical conditions with the exception of clinorotation. RESULTS: Simulated microgravity had different effects on each lung cancer cell line. In A549 cells, the proliferation rate of the clinostat group (2.267 ± 0.010) after exposure to microgravity did not differ from that of the control group (2.271 ± 0.020). However, in H1703 cells, the proliferation rates of the clinostat group (0.534 6 0.021) was lower than that of the control group (1.082 ± 0.021). The migratory ability of both A549 [remnant cell-free area: 33% (clinostat) vs. 78% (control)] and H1703 cells [40% (clinostat) vs. 68% (control)] were increased after exposure to microgravity. The results of the molecular changes in biomarkers after exposure to microgravity are preliminary. DISCUSSION: Simulated microgravity had different effects on the proliferation and migration of different lung cancer cell lines.

Original languageEnglish
Pages (from-to)82-89
Number of pages8
JournalAerospace medicine and human performance
Volume88
Issue number2
DOIs
Publication statusPublished - 2017 Feb 1

Fingerprint

Weightlessness
Non-Small Cell Lung Carcinoma
Cell Movement
Cell Proliferation
Lung Neoplasms
Cell Line
Control Groups
Cell Communication
Squamous Cell Carcinoma
Adenocarcinoma
Biomarkers
Neoplasm Metastasis
Technology

Keywords

  • Lung cancer cells
  • Migration
  • Proliferation
  • Simulated microgravity

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Public Health, Environmental and Occupational Health

Cite this

Simulated microgravity effects on nonsmall cell lung cancer cell proliferation and migration. / Chung, Jae Ho; Ahn, Chi Bum; Son, Kuk Hui; Yi, Eunjue; Son, Ho Sung; Kim, Han Sung; Lee, Sung Ho.

In: Aerospace medicine and human performance, Vol. 88, No. 2, 01.02.2017, p. 82-89.

Research output: Contribution to journalArticle

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