Simulated microgravity with floating environment promotes migration of non-small cell lung cancers

Chi Bum Ahn, Ji Hyun Lee, Dae Geun Han, Hyun Wook Kang, Sung Ho Lee, Jae Ik Lee, Kuk Hui Son, Jin Woo Lee

Research output: Contribution to journalArticle

Abstract

A migration of cancer is one of the most important factors affecting cancer therapy. Particularly, a cancer migration study in a microgravity environment has gained attention as a tool for developing cancer therapy. In this study, we evaluated the proliferation and migration of two types (adenocarcinoma A549, squamous cell carcinoma H1703) of non-small cell lung cancers (NSCLC) in a floating environment with microgravity. When we measured proliferation of two NSCLCs in the microgravity (MG) and ground-gravity (CONT), although initial cell adhesion in MG was low, a normalized proliferation rate of A549 in MG was higher than that in CONT. Wound healing results of A549 and H1703 showed rapid recovery in MG; particularly, the migration rate of A549 was faster than that of H1703 both the normal and low proliferating conditions. Gene expression results showed that the microgravity accelerated the migration of NSCLC. Both A549 and H1703 in MG highly expressed the migration-related genes MMP-2, MMP-9, TIMP-1, and TIMP-2 compared to CONT at 24 h. Furthermore, analysis of MMP-2 protein synthesis revealed weaker metastatic performance of H1703 than that of A549. Therefore, the simulated microgravity based cancer culture environment will be a potential for migration and metastasis studies of lung cancers.

Original languageEnglish
Article number14553
JournalScientific reports
Volume9
Issue number1
DOIs
Publication statusPublished - 2019 Dec 1

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Weightlessness
Non-Small Cell Lung Carcinoma
Matrix Metalloproteinases
Neoplasms
Tissue Inhibitor of Metalloproteinase-2
Tissue Inhibitor of Metalloproteinase-1
Gravitation
Cell Adhesion
Wound Healing
Squamous Cell Carcinoma
Lung Neoplasms
Adenocarcinoma
Neoplasm Metastasis
Gene Expression

ASJC Scopus subject areas

  • General

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Simulated microgravity with floating environment promotes migration of non-small cell lung cancers. / Ahn, Chi Bum; Lee, Ji Hyun; Han, Dae Geun; Kang, Hyun Wook; Lee, Sung Ho; Lee, Jae Ik; Son, Kuk Hui; Lee, Jin Woo.

In: Scientific reports, Vol. 9, No. 1, 14553, 01.12.2019.

Research output: Contribution to journalArticle

Ahn, Chi Bum ; Lee, Ji Hyun ; Han, Dae Geun ; Kang, Hyun Wook ; Lee, Sung Ho ; Lee, Jae Ik ; Son, Kuk Hui ; Lee, Jin Woo. / Simulated microgravity with floating environment promotes migration of non-small cell lung cancers. In: Scientific reports. 2019 ; Vol. 9, No. 1.
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