Simultaneous Detection of Dual Prostate Specific Antigens Using Surface-Enhanced Raman Scattering-Based Immunoassay for Accurate Diagnosis of Prostate Cancer

Ziyi Cheng; Namhyun Choi; Rui Wang; Sangyeop Lee; Kyung Chul Moon; Soo Young Yoon; Lingxin Chen; Jaebum Choo

doi:10.1021/acsnano.7b01536

Simultaneous Detection of Dual Prostate Specific Antigens Using Surface-Enhanced Raman Scattering-Based Immunoassay for Accurate Diagnosis of Prostate Cancer

Ziyi Cheng, Namhyun Choi, Rui Wang, Sangyeop Lee, Kyung Chul Moon, Soo Young Yoon, Lingxin Chen, Jaebum Choo

Department of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

243 Citations (Scopus)

Abstract

Accurate analysis of specific biomarkers in clinical serum is essential for early diagnosis and treatment of cancer. Here, a surface-enhanced Raman scattering (SERS)-based immunoassay, using magnetic beads and SERS nano tags, was developed for the determination of free to total (f/t) prostate specific antigen (PSA) ratio to improve the diagnostic performance of prostate cancer. To assess the clinical applicability of the proposed method, SERS-based assays for the simultaneous detection of dual PSA markers, free PSA (f-PSA) and complexed PSA (c-PSA), were performed for clinical samples in the gray zone between 4.0 and 10.0 ng/mL. Our assay results for f/t PSA ratio showed a good linear correlation with those measured using the electrochemiluminescence (ECL) system installed in the clinical laboratory of the University Hospital. In addition, the simultaneous assay provided better precision than parallel assays for the detection of f-PSA and c-PSA in 13 clinical serum samples. Therefore, our SERS-based assay for simultaneous detection of dual PSA markers in clinical fluids has strong potential for application in the accurate diagnosis of prostate cancer.

Original language	English
Pages (from-to)	4926-4933
Number of pages	8
Journal	ACS nano
Volume	11
Issue number	5
DOIs	https://doi.org/10.1021/acsnano.7b01536
Publication status	Published - 2017 May 23

Keywords

SERS-based immunoassay
free to total PSA ratio
multiplex detection
prostate cancer
simultaneous detection

ASJC Scopus subject areas

Materials Science(all)
Engineering(all)
Physics and Astronomy(all)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1021/acsnano.7b01536

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title = "Simultaneous Detection of Dual Prostate Specific Antigens Using Surface-Enhanced Raman Scattering-Based Immunoassay for Accurate Diagnosis of Prostate Cancer",

abstract = "Accurate analysis of specific biomarkers in clinical serum is essential for early diagnosis and treatment of cancer. Here, a surface-enhanced Raman scattering (SERS)-based immunoassay, using magnetic beads and SERS nano tags, was developed for the determination of free to total (f/t) prostate specific antigen (PSA) ratio to improve the diagnostic performance of prostate cancer. To assess the clinical applicability of the proposed method, SERS-based assays for the simultaneous detection of dual PSA markers, free PSA (f-PSA) and complexed PSA (c-PSA), were performed for clinical samples in the gray zone between 4.0 and 10.0 ng/mL. Our assay results for f/t PSA ratio showed a good linear correlation with those measured using the electrochemiluminescence (ECL) system installed in the clinical laboratory of the University Hospital. In addition, the simultaneous assay provided better precision than parallel assays for the detection of f-PSA and c-PSA in 13 clinical serum samples. Therefore, our SERS-based assay for simultaneous detection of dual PSA markers in clinical fluids has strong potential for application in the accurate diagnosis of prostate cancer.",

keywords = "SERS-based immunoassay, free to total PSA ratio, multiplex detection, prostate cancer, simultaneous detection",

author = "Ziyi Cheng and Namhyun Choi and Rui Wang and Sangyeop Lee and Moon, {Kyung Chul} and Yoon, {Soo Young} and Lingxin Chen and Jaebum Choo",

note = "Funding Information: The National Research Foundation of Korea supported this work through grant numbers R11-2008-0061852 and K20904000004-12A0500-00410. The Nano Material Technology Development Program also supported this work, through the National Research Foundation of Korea, funded by the Ministry of Education, Science, and Technology (grant number 2012035286). This work was also partially supported by the Korea Health Industry Development Institute (KHIDI) funded by the Ministry of Health and Welfare, Korea (HI16C2129). L.C. thanks the National Natural Science Foundation of China (21575159) for financial support. Publisher Copyright: {\textcopyright} 2017 American Chemical Society.",

year = "2017",

month = may,

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doi = "10.1021/acsnano.7b01536",

language = "English",

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T1 - Simultaneous Detection of Dual Prostate Specific Antigens Using Surface-Enhanced Raman Scattering-Based Immunoassay for Accurate Diagnosis of Prostate Cancer

AU - Cheng, Ziyi

AU - Choi, Namhyun

AU - Wang, Rui

AU - Lee, Sangyeop

AU - Moon, Kyung Chul

AU - Yoon, Soo Young

AU - Chen, Lingxin

AU - Choo, Jaebum

N1 - Funding Information: The National Research Foundation of Korea supported this work through grant numbers R11-2008-0061852 and K20904000004-12A0500-00410. The Nano Material Technology Development Program also supported this work, through the National Research Foundation of Korea, funded by the Ministry of Education, Science, and Technology (grant number 2012035286). This work was also partially supported by the Korea Health Industry Development Institute (KHIDI) funded by the Ministry of Health and Welfare, Korea (HI16C2129). L.C. thanks the National Natural Science Foundation of China (21575159) for financial support. Publisher Copyright: © 2017 American Chemical Society.

PY - 2017/5/23

Y1 - 2017/5/23

N2 - Accurate analysis of specific biomarkers in clinical serum is essential for early diagnosis and treatment of cancer. Here, a surface-enhanced Raman scattering (SERS)-based immunoassay, using magnetic beads and SERS nano tags, was developed for the determination of free to total (f/t) prostate specific antigen (PSA) ratio to improve the diagnostic performance of prostate cancer. To assess the clinical applicability of the proposed method, SERS-based assays for the simultaneous detection of dual PSA markers, free PSA (f-PSA) and complexed PSA (c-PSA), were performed for clinical samples in the gray zone between 4.0 and 10.0 ng/mL. Our assay results for f/t PSA ratio showed a good linear correlation with those measured using the electrochemiluminescence (ECL) system installed in the clinical laboratory of the University Hospital. In addition, the simultaneous assay provided better precision than parallel assays for the detection of f-PSA and c-PSA in 13 clinical serum samples. Therefore, our SERS-based assay for simultaneous detection of dual PSA markers in clinical fluids has strong potential for application in the accurate diagnosis of prostate cancer.

AB - Accurate analysis of specific biomarkers in clinical serum is essential for early diagnosis and treatment of cancer. Here, a surface-enhanced Raman scattering (SERS)-based immunoassay, using magnetic beads and SERS nano tags, was developed for the determination of free to total (f/t) prostate specific antigen (PSA) ratio to improve the diagnostic performance of prostate cancer. To assess the clinical applicability of the proposed method, SERS-based assays for the simultaneous detection of dual PSA markers, free PSA (f-PSA) and complexed PSA (c-PSA), were performed for clinical samples in the gray zone between 4.0 and 10.0 ng/mL. Our assay results for f/t PSA ratio showed a good linear correlation with those measured using the electrochemiluminescence (ECL) system installed in the clinical laboratory of the University Hospital. In addition, the simultaneous assay provided better precision than parallel assays for the detection of f-PSA and c-PSA in 13 clinical serum samples. Therefore, our SERS-based assay for simultaneous detection of dual PSA markers in clinical fluids has strong potential for application in the accurate diagnosis of prostate cancer.

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Simultaneous Detection of Dual Prostate Specific Antigens Using Surface-Enhanced Raman Scattering-Based Immunoassay for Accurate Diagnosis of Prostate Cancer

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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