Simultaneous determination of bioactive phenolic compounds in the stem extract of Rhus verniciflua stokes by high performance liquid chromatography

Sun A. Kim, Seung Hyun Kim, In Sook Kim, Dongho Lee, Mi Sook Dong, Chun Soo Na, Nguyen Xuan Nhiem, Hye Hyun Yoo

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A simple, sensitive, and precise reversed-phase liquid chromatographic method was developed for the quantitative determination of 4 bioactive phenolic compounds (gallic acid, fustin, fisetin, and sulfuretin) from the stem extract of Rhus verniciflua stokes. Chromatographic analysis was performed on a Capcell Pak C18 column (150 × 4.6 mm, 3 μm) with a mobile phase consisting of 0.1% formic acid and 90% acetonitrile at a flow rate of 1 mL/min. Quantitation was performed using a UV-vis detector at 260 nm. The method was validated in terms of selectivity, linearity, accuracy, precision, and recovery. Excellent linear behavior was observed over the investigated concentration range (10-500 μ/mL for gallic acid, fustin, and fisetin; 0.5-100 μ/mL for sulfuretin) with correlation coefficient (r2) values >0.99. The intra- and inter-day precision over the concentration range of compounds was less than 6.65% (relative standard deviation) and the accuracy was between 92.42% and 103.62%. The mean recoveries for all the analytes were more than 92.18%. This method was successfully applied for the analysis of bioactive phenolic compounds in the R. verniciflua extracts.

Original languageEnglish
Pages (from-to)3813-3819
Number of pages7
JournalFood Chemistry
Issue number4
Publication statusPublished - 2013 Oct 1



  • HPLC
  • Phenolic compounds
  • Rhus verniciflua stokes
  • Validation

ASJC Scopus subject areas

  • Food Science
  • Analytical Chemistry

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