Abstract
In this study, a rapid method for simultaneous detection of ethyl carbamate (EC) and urea in Korean rice wine was developed. To achieve quantitative analysis of EC and urea, the conditions for Ultra-performance liquid chromatography (UPLC) separation and atmospheric-pressure chemical ionization tandem mass spectrometry (APCI-MS/MS) detection were first optimized. Under the established conditions, the detection limit, relative standard deviation and linear range were 2.83 μg/L, 3.75–5.96%, and 0.01–10.0 mg/L, respectively, for urea; the corresponding values were 0.17 μg/L, 1.06–4.01%, and 1.0–50.0 μg/L, respectively, for EC. The correlation between the contents of EC and its precursor urea was determined under specific pH (3.5 and 4.5) and temperature (4, 25, and 50 °C) conditions using the developed method. As a result, EC content was increased with greater temperature and lower pH. In Korean rice wine, urea was detected 0.19–1.37 mg/L and EC was detected 2.0–7.7 μg/L. The method developed in this study, which has the advantages of simplified sample preparation, low detection limits, and good selectivity, was successfully applied for the rapid analysis of EC and urea.
| Original language | English |
|---|---|
| Pages (from-to) | 44-49 |
| Number of pages | 6 |
| Journal | Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences |
| Volume | 1065-1066 |
| DOIs | |
| Publication status | Published - 2017 Oct 15 |
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Keywords
- Atmospheric pressure chemical ionization
- Ethyl carbamate
- Korean rice wine
- Mass spectrometry
- Ultra performance liquid chromatography
- Urea
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Clinical Biochemistry
- Cell Biology
Cite this
Simultaneous determination of ethyl carbamate and urea in Korean rice wine by ultra-performance liquid chromatography coupled with mass spectrometric detection. / Lee, Gyeong hweon; Bang, Dae young; Lim, Jung hoon; Yoon, Seok min; Yea, Myeong Jai; Chi, Young Min.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 1065-1066, 15.10.2017, p. 44-49.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Simultaneous determination of ethyl carbamate and urea in Korean rice wine by ultra-performance liquid chromatography coupled with mass spectrometric detection
AU - Lee, Gyeong hweon
AU - Bang, Dae young
AU - Lim, Jung hoon
AU - Yoon, Seok min
AU - Yea, Myeong Jai
AU - Chi, Young Min
PY - 2017/10/15
Y1 - 2017/10/15
N2 - In this study, a rapid method for simultaneous detection of ethyl carbamate (EC) and urea in Korean rice wine was developed. To achieve quantitative analysis of EC and urea, the conditions for Ultra-performance liquid chromatography (UPLC) separation and atmospheric-pressure chemical ionization tandem mass spectrometry (APCI-MS/MS) detection were first optimized. Under the established conditions, the detection limit, relative standard deviation and linear range were 2.83 μg/L, 3.75–5.96%, and 0.01–10.0 mg/L, respectively, for urea; the corresponding values were 0.17 μg/L, 1.06–4.01%, and 1.0–50.0 μg/L, respectively, for EC. The correlation between the contents of EC and its precursor urea was determined under specific pH (3.5 and 4.5) and temperature (4, 25, and 50 °C) conditions using the developed method. As a result, EC content was increased with greater temperature and lower pH. In Korean rice wine, urea was detected 0.19–1.37 mg/L and EC was detected 2.0–7.7 μg/L. The method developed in this study, which has the advantages of simplified sample preparation, low detection limits, and good selectivity, was successfully applied for the rapid analysis of EC and urea.
AB - In this study, a rapid method for simultaneous detection of ethyl carbamate (EC) and urea in Korean rice wine was developed. To achieve quantitative analysis of EC and urea, the conditions for Ultra-performance liquid chromatography (UPLC) separation and atmospheric-pressure chemical ionization tandem mass spectrometry (APCI-MS/MS) detection were first optimized. Under the established conditions, the detection limit, relative standard deviation and linear range were 2.83 μg/L, 3.75–5.96%, and 0.01–10.0 mg/L, respectively, for urea; the corresponding values were 0.17 μg/L, 1.06–4.01%, and 1.0–50.0 μg/L, respectively, for EC. The correlation between the contents of EC and its precursor urea was determined under specific pH (3.5 and 4.5) and temperature (4, 25, and 50 °C) conditions using the developed method. As a result, EC content was increased with greater temperature and lower pH. In Korean rice wine, urea was detected 0.19–1.37 mg/L and EC was detected 2.0–7.7 μg/L. The method developed in this study, which has the advantages of simplified sample preparation, low detection limits, and good selectivity, was successfully applied for the rapid analysis of EC and urea.
KW - Atmospheric pressure chemical ionization
KW - Ethyl carbamate
KW - Korean rice wine
KW - Mass spectrometry
KW - Ultra performance liquid chromatography
KW - Urea
UR - http://www.scopus.com/inward/record.url?scp=85029715614&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85029715614&partnerID=8YFLogxK
U2 - 10.1016/j.jchromb.2017.09.011
DO - 10.1016/j.jchromb.2017.09.011
M3 - Article
C2 - 28946124
AN - SCOPUS:85029715614
VL - 1065-1066
SP - 44
EP - 49
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
SN - 1570-0232
ER -