Simultaneous determination of ethyl carbamate and urea in Korean rice wine by ultra-performance liquid chromatography coupled with mass spectrometric detection

Gyeong hweon Lee, Dae young Bang, Jung hoon Lim, Seok min Yoon, Myeong Jai Yea, Young Min Chi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In this study, a rapid method for simultaneous detection of ethyl carbamate (EC) and urea in Korean rice wine was developed. To achieve quantitative analysis of EC and urea, the conditions for Ultra-performance liquid chromatography (UPLC) separation and atmospheric-pressure chemical ionization tandem mass spectrometry (APCI-MS/MS) detection were first optimized. Under the established conditions, the detection limit, relative standard deviation and linear range were 2.83 μg/L, 3.75–5.96%, and 0.01–10.0 mg/L, respectively, for urea; the corresponding values were 0.17 μg/L, 1.06–4.01%, and 1.0–50.0 μg/L, respectively, for EC. The correlation between the contents of EC and its precursor urea was determined under specific pH (3.5 and 4.5) and temperature (4, 25, and 50 °C) conditions using the developed method. As a result, EC content was increased with greater temperature and lower pH. In Korean rice wine, urea was detected 0.19–1.37 mg/L and EC was detected 2.0–7.7 μg/L. The method developed in this study, which has the advantages of simplified sample preparation, low detection limits, and good selectivity, was successfully applied for the rapid analysis of EC and urea.

Original languageEnglish
Pages (from-to)44-49
Number of pages6
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume1065-1066
DOIs
Publication statusPublished - 2017 Oct 15

Fingerprint

Wine
Urethane
Liquid chromatography
Liquid Chromatography
Urea
Limit of Detection
Temperature
Atmospheric Pressure
Tandem Mass Spectrometry
Oryza
Atmospheric pressure
Ionization
Mass spectrometry
Chemical analysis

Keywords

  • Atmospheric pressure chemical ionization
  • Ethyl carbamate
  • Korean rice wine
  • Mass spectrometry
  • Ultra performance liquid chromatography
  • Urea

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

Cite this

Simultaneous determination of ethyl carbamate and urea in Korean rice wine by ultra-performance liquid chromatography coupled with mass spectrometric detection. / Lee, Gyeong hweon; Bang, Dae young; Lim, Jung hoon; Yoon, Seok min; Yea, Myeong Jai; Chi, Young Min.

In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 1065-1066, 15.10.2017, p. 44-49.

Research output: Contribution to journalArticle

@article{cd9591a561cb4f19b52f54681f3e715e,
title = "Simultaneous determination of ethyl carbamate and urea in Korean rice wine by ultra-performance liquid chromatography coupled with mass spectrometric detection",
abstract = "In this study, a rapid method for simultaneous detection of ethyl carbamate (EC) and urea in Korean rice wine was developed. To achieve quantitative analysis of EC and urea, the conditions for Ultra-performance liquid chromatography (UPLC) separation and atmospheric-pressure chemical ionization tandem mass spectrometry (APCI-MS/MS) detection were first optimized. Under the established conditions, the detection limit, relative standard deviation and linear range were 2.83 μg/L, 3.75–5.96{\%}, and 0.01–10.0 mg/L, respectively, for urea; the corresponding values were 0.17 μg/L, 1.06–4.01{\%}, and 1.0–50.0 μg/L, respectively, for EC. The correlation between the contents of EC and its precursor urea was determined under specific pH (3.5 and 4.5) and temperature (4, 25, and 50 °C) conditions using the developed method. As a result, EC content was increased with greater temperature and lower pH. In Korean rice wine, urea was detected 0.19–1.37 mg/L and EC was detected 2.0–7.7 μg/L. The method developed in this study, which has the advantages of simplified sample preparation, low detection limits, and good selectivity, was successfully applied for the rapid analysis of EC and urea.",
keywords = "Atmospheric pressure chemical ionization, Ethyl carbamate, Korean rice wine, Mass spectrometry, Ultra performance liquid chromatography, Urea",
author = "Lee, {Gyeong hweon} and Bang, {Dae young} and Lim, {Jung hoon} and Yoon, {Seok min} and Yea, {Myeong Jai} and Chi, {Young Min}",
year = "2017",
month = "10",
day = "15",
doi = "10.1016/j.jchromb.2017.09.011",
language = "English",
volume = "1065-1066",
pages = "44--49",
journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",
issn = "1570-0232",
publisher = "Elsevier",

}

TY - JOUR

T1 - Simultaneous determination of ethyl carbamate and urea in Korean rice wine by ultra-performance liquid chromatography coupled with mass spectrometric detection

AU - Lee, Gyeong hweon

AU - Bang, Dae young

AU - Lim, Jung hoon

AU - Yoon, Seok min

AU - Yea, Myeong Jai

AU - Chi, Young Min

PY - 2017/10/15

Y1 - 2017/10/15

N2 - In this study, a rapid method for simultaneous detection of ethyl carbamate (EC) and urea in Korean rice wine was developed. To achieve quantitative analysis of EC and urea, the conditions for Ultra-performance liquid chromatography (UPLC) separation and atmospheric-pressure chemical ionization tandem mass spectrometry (APCI-MS/MS) detection were first optimized. Under the established conditions, the detection limit, relative standard deviation and linear range were 2.83 μg/L, 3.75–5.96%, and 0.01–10.0 mg/L, respectively, for urea; the corresponding values were 0.17 μg/L, 1.06–4.01%, and 1.0–50.0 μg/L, respectively, for EC. The correlation between the contents of EC and its precursor urea was determined under specific pH (3.5 and 4.5) and temperature (4, 25, and 50 °C) conditions using the developed method. As a result, EC content was increased with greater temperature and lower pH. In Korean rice wine, urea was detected 0.19–1.37 mg/L and EC was detected 2.0–7.7 μg/L. The method developed in this study, which has the advantages of simplified sample preparation, low detection limits, and good selectivity, was successfully applied for the rapid analysis of EC and urea.

AB - In this study, a rapid method for simultaneous detection of ethyl carbamate (EC) and urea in Korean rice wine was developed. To achieve quantitative analysis of EC and urea, the conditions for Ultra-performance liquid chromatography (UPLC) separation and atmospheric-pressure chemical ionization tandem mass spectrometry (APCI-MS/MS) detection were first optimized. Under the established conditions, the detection limit, relative standard deviation and linear range were 2.83 μg/L, 3.75–5.96%, and 0.01–10.0 mg/L, respectively, for urea; the corresponding values were 0.17 μg/L, 1.06–4.01%, and 1.0–50.0 μg/L, respectively, for EC. The correlation between the contents of EC and its precursor urea was determined under specific pH (3.5 and 4.5) and temperature (4, 25, and 50 °C) conditions using the developed method. As a result, EC content was increased with greater temperature and lower pH. In Korean rice wine, urea was detected 0.19–1.37 mg/L and EC was detected 2.0–7.7 μg/L. The method developed in this study, which has the advantages of simplified sample preparation, low detection limits, and good selectivity, was successfully applied for the rapid analysis of EC and urea.

KW - Atmospheric pressure chemical ionization

KW - Ethyl carbamate

KW - Korean rice wine

KW - Mass spectrometry

KW - Ultra performance liquid chromatography

KW - Urea

UR - http://www.scopus.com/inward/record.url?scp=85029715614&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85029715614&partnerID=8YFLogxK

U2 - 10.1016/j.jchromb.2017.09.011

DO - 10.1016/j.jchromb.2017.09.011

M3 - Article

C2 - 28946124

AN - SCOPUS:85029715614

VL - 1065-1066

SP - 44

EP - 49

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

SN - 1570-0232

ER -