Simultaneous optogenetic stimulation of individual pharyngeal neurons and monitoring of feeding behavior in intact C. elegans

Nicholas F. Trojanowski, Christopher Fang-Yen

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Citations (Scopus)

Abstract

Optogenetic approaches have proven powerful for examining the role of neural circuits in generating behaviors, especially in systems where electrophysiological manipulation is not possible. Here we describe a method for optogenetically manipulating single pharyngeal neurons in intact C. elegans while monitoring pharyngeal behavior. This approach provides bidirectional and dynamic control of pharyngeal neural activity simultaneously with a behavioral readout and has allowed us to test hypotheses about the roles of individual pharyngeal neurons in regulating feeding behavior.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages105-119
Number of pages15
Volume1327
DOIs
Publication statusPublished - 2015

Publication series

NameMethods in Molecular Biology
Volume1327
ISSN (Print)10643745

Fingerprint

Optogenetics
Feeding Behavior
Neurons

Keywords

  • C. elegans
  • Feeding behavior
  • In vivo optogenetics
  • Optogenetics
  • Pharynx
  • Small circuits

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Trojanowski, N. F., & Fang-Yen, C. (2015). Simultaneous optogenetic stimulation of individual pharyngeal neurons and monitoring of feeding behavior in intact C. elegans. In Methods in Molecular Biology (Vol. 1327, pp. 105-119). (Methods in Molecular Biology; Vol. 1327). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-2842-2_9

Simultaneous optogenetic stimulation of individual pharyngeal neurons and monitoring of feeding behavior in intact C. elegans. / Trojanowski, Nicholas F.; Fang-Yen, Christopher.

Methods in Molecular Biology. Vol. 1327 Humana Press Inc., 2015. p. 105-119 (Methods in Molecular Biology; Vol. 1327).

Research output: Chapter in Book/Report/Conference proceedingChapter

Trojanowski, NF & Fang-Yen, C 2015, Simultaneous optogenetic stimulation of individual pharyngeal neurons and monitoring of feeding behavior in intact C. elegans. in Methods in Molecular Biology. vol. 1327, Methods in Molecular Biology, vol. 1327, Humana Press Inc., pp. 105-119. https://doi.org/10.1007/978-1-4939-2842-2_9
Trojanowski NF, Fang-Yen C. Simultaneous optogenetic stimulation of individual pharyngeal neurons and monitoring of feeding behavior in intact C. elegans. In Methods in Molecular Biology. Vol. 1327. Humana Press Inc. 2015. p. 105-119. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-2842-2_9
Trojanowski, Nicholas F. ; Fang-Yen, Christopher. / Simultaneous optogenetic stimulation of individual pharyngeal neurons and monitoring of feeding behavior in intact C. elegans. Methods in Molecular Biology. Vol. 1327 Humana Press Inc., 2015. pp. 105-119 (Methods in Molecular Biology).
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