Abstract
Optogenetic approaches have proven powerful for examining the role of neural circuits in generating behaviors, especially in systems where electrophysiological manipulation is not possible. Here we describe a method for optogenetically manipulating single pharyngeal neurons in intact C. elegans while monitoring pharyngeal behavior. This approach provides bidirectional and dynamic control of pharyngeal neural activity simultaneously with a behavioral readout and has allowed us to test hypotheses about the roles of individual pharyngeal neurons in regulating feeding behavior.
| Original language | English |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press Inc. |
| Pages | 105-119 |
| Number of pages | 15 |
| DOIs | |
| Publication status | Published - 2015 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1327 |
| ISSN (Print) | 1064-3745 |
Keywords
- C. elegans
- Feeding behavior
- In vivo optogenetics
- Optogenetics
- Pharynx
- Small circuits
ASJC Scopus subject areas
- Molecular Biology
- Genetics
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Trojanowski, N. F., & Fang-Yen, C. (2015). Simultaneous optogenetic stimulation of individual pharyngeal neurons and monitoring of feeding behavior in intact C. elegans. In Methods in Molecular Biology (pp. 105-119). (Methods in Molecular Biology; Vol. 1327). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-2842-2_9