Abstract
Optogenetic approaches have proven powerful for examining the role of neural circuits in generating behaviors, especially in systems where electrophysiological manipulation is not possible. Here we describe a method for optogenetically manipulating single pharyngeal neurons in intact C. elegans while monitoring pharyngeal behavior. This approach provides bidirectional and dynamic control of pharyngeal neural activity simultaneously with a behavioral readout and has allowed us to test hypotheses about the roles of individual pharyngeal neurons in regulating feeding behavior.
| Original language | English |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press Inc. |
| Pages | 105-119 |
| Number of pages | 15 |
| Volume | 1327 |
| DOIs | |
| Publication status | Published - 2015 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1327 |
| ISSN (Print) | 10643745 |
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Keywords
- C. elegans
- Feeding behavior
- In vivo optogenetics
- Optogenetics
- Pharynx
- Small circuits
ASJC Scopus subject areas
- Molecular Biology
- Genetics
Cite this
Simultaneous optogenetic stimulation of individual pharyngeal neurons and monitoring of feeding behavior in intact C. elegans. / Trojanowski, Nicholas F.; Fang-Yen, Christopher.
Methods in Molecular Biology. Vol. 1327 Humana Press Inc., 2015. p. 105-119 (Methods in Molecular Biology; Vol. 1327).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Simultaneous optogenetic stimulation of individual pharyngeal neurons and monitoring of feeding behavior in intact C. elegans
AU - Trojanowski, Nicholas F.
AU - Fang-Yen, Christopher
PY - 2015
Y1 - 2015
N2 - Optogenetic approaches have proven powerful for examining the role of neural circuits in generating behaviors, especially in systems where electrophysiological manipulation is not possible. Here we describe a method for optogenetically manipulating single pharyngeal neurons in intact C. elegans while monitoring pharyngeal behavior. This approach provides bidirectional and dynamic control of pharyngeal neural activity simultaneously with a behavioral readout and has allowed us to test hypotheses about the roles of individual pharyngeal neurons in regulating feeding behavior.
AB - Optogenetic approaches have proven powerful for examining the role of neural circuits in generating behaviors, especially in systems where electrophysiological manipulation is not possible. Here we describe a method for optogenetically manipulating single pharyngeal neurons in intact C. elegans while monitoring pharyngeal behavior. This approach provides bidirectional and dynamic control of pharyngeal neural activity simultaneously with a behavioral readout and has allowed us to test hypotheses about the roles of individual pharyngeal neurons in regulating feeding behavior.
KW - C. elegans
KW - Feeding behavior
KW - In vivo optogenetics
KW - Optogenetics
KW - Pharynx
KW - Small circuits
UR - http://www.scopus.com/inward/record.url?scp=84942866277&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84942866277&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-2842-2_9
DO - 10.1007/978-1-4939-2842-2_9
M3 - Chapter
C2 - 26423971
AN - SCOPUS:84942866277
VL - 1327
T3 - Methods in Molecular Biology
SP - 105
EP - 119
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -